Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut

ABSTRACT The Salmonella genus comprises a group of pathogens associated with illnesses ranging from gastroenteritis to typhoid fever. We performed an in silico analysis of comparatively reannotated Salmonella genomes to identify genomic signatures indicative of disease potential. By removing numerou...

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Autores principales:	Sean-Paul Nuccio, Andreas J. Bäumler
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/c63b4cd076dc4063abff3672cb25024e
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spelling	oai:doaj.org-article:c63b4cd076dc4063abff3672cb25024e2021-11-15T15:45:13ZComparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut10.1128/mBio.00929-142150-7511https://doaj.org/article/c63b4cd076dc4063abff3672cb25024e2014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00929-14https://doaj.org/toc/2150-7511ABSTRACT The Salmonella genus comprises a group of pathogens associated with illnesses ranging from gastroenteritis to typhoid fever. We performed an in silico analysis of comparatively reannotated Salmonella genomes to identify genomic signatures indicative of disease potential. By removing numerous annotation inconsistencies and inaccuracies, the process of reannotation identified a network of 469 genes involved in central anaerobic metabolism, which was intact in genomes of gastrointestinal pathogens but degrading in genomes of extraintestinal pathogens. This large network contained pathways that enable gastrointestinal pathogens to utilize inflammation-derived nutrients as well as many of the biochemical reactions used for the enrichment and biochemical discrimination of Salmonella serovars. Thus, comparative genome analysis identifies a metabolic network that provides clues about the strategies for nutrient acquisition and utilization that are characteristic of gastrointestinal pathogens. IMPORTANCE While some Salmonella serovars cause infections that remain localized to the gut, others disseminate throughout the body. Here, we compared Salmonella genomes to identify characteristics that distinguish gastrointestinal from extraintestinal pathogens. We identified a large metabolic network that is functional in gastrointestinal pathogens but decaying in extraintestinal pathogens. While taxonomists have used traits from this network empirically for many decades for the enrichment and biochemical discrimination of Salmonella serovars, our findings suggest that it is part of a “business plan” for growth in the inflamed gastrointestinal tract. By identifying a large metabolic network characteristic of Salmonella serovars associated with gastroenteritis, our in silico analysis provides a blueprint for potential strategies to utilize inflammation-derived nutrients and edge out competing gut microbes.Sean-Paul NuccioAndreas J. BäumlerAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Sean-Paul Nuccio Andreas J. Bäumler Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut
description	ABSTRACT The Salmonella genus comprises a group of pathogens associated with illnesses ranging from gastroenteritis to typhoid fever. We performed an in silico analysis of comparatively reannotated Salmonella genomes to identify genomic signatures indicative of disease potential. By removing numerous annotation inconsistencies and inaccuracies, the process of reannotation identified a network of 469 genes involved in central anaerobic metabolism, which was intact in genomes of gastrointestinal pathogens but degrading in genomes of extraintestinal pathogens. This large network contained pathways that enable gastrointestinal pathogens to utilize inflammation-derived nutrients as well as many of the biochemical reactions used for the enrichment and biochemical discrimination of Salmonella serovars. Thus, comparative genome analysis identifies a metabolic network that provides clues about the strategies for nutrient acquisition and utilization that are characteristic of gastrointestinal pathogens. IMPORTANCE While some Salmonella serovars cause infections that remain localized to the gut, others disseminate throughout the body. Here, we compared Salmonella genomes to identify characteristics that distinguish gastrointestinal from extraintestinal pathogens. We identified a large metabolic network that is functional in gastrointestinal pathogens but decaying in extraintestinal pathogens. While taxonomists have used traits from this network empirically for many decades for the enrichment and biochemical discrimination of Salmonella serovars, our findings suggest that it is part of a “business plan” for growth in the inflamed gastrointestinal tract. By identifying a large metabolic network characteristic of Salmonella serovars associated with gastroenteritis, our in silico analysis provides a blueprint for potential strategies to utilize inflammation-derived nutrients and edge out competing gut microbes.
format	article
author	Sean-Paul Nuccio Andreas J. Bäumler
author_facet	Sean-Paul Nuccio Andreas J. Bäumler
author_sort	Sean-Paul Nuccio
title	Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut
title_short	Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut
title_full	Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut
title_fullStr	Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut
title_full_unstemmed	Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut
title_sort	comparative analysis of <italic toggle="yes">salmonella</italic> genomes identifies a metabolic network for escalating growth in the inflamed gut
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/c63b4cd076dc4063abff3672cb25024e
work_keys_str_mv	AT seanpaulnuccio comparativeanalysisofitalictoggleyessalmonellaitalicgenomesidentifiesametabolicnetworkforescalatinggrowthintheinflamedgut AT andreasjbaumler comparativeanalysisofitalictoggleyessalmonellaitalicgenomesidentifiesametabolicnetworkforescalatinggrowthintheinflamedgut
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Comparative Analysis of <italic toggle="yes">Salmonella</italic> Genomes Identifies a Metabolic Network for Escalating Growth in the Inflamed Gut

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