Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations

Fujita et al. investigate the use of oligoribonucleotides, proteins, and ribonucleoprotein complexes as sequence-specific blockers of DNA extension by DNA polymerases. They demonstrate the value of proteins and ribonucleoprotein complexes as blockers in low-temperature isothermal DNA amplification r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Toshitsugu Fujita, Shoko Nagata, Hodaka Fujii
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c640dd47061a477aa32a90e8e90fa556
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c640dd47061a477aa32a90e8e90fa556
record_format	dspace
spelling	oai:doaj.org-article:c640dd47061a477aa32a90e8e90fa5562021-12-02T15:10:45ZProtein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations10.1038/s42003-021-02503-52399-3642https://doaj.org/article/c640dd47061a477aa32a90e8e90fa5562021-08-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02503-5https://doaj.org/toc/2399-3642Fujita et al. investigate the use of oligoribonucleotides, proteins, and ribonucleoprotein complexes as sequence-specific blockers of DNA extension by DNA polymerases. They demonstrate the value of proteins and ribonucleoprotein complexes as blockers in low-temperature isothermal DNA amplification reactions for discrimination of nucleotide and epigenetic differences.Toshitsugu FujitaShoko NagataHodaka FujiiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Toshitsugu Fujita Shoko Nagata Hodaka Fujii Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
description	Fujita et al. investigate the use of oligoribonucleotides, proteins, and ribonucleoprotein complexes as sequence-specific blockers of DNA extension by DNA polymerases. They demonstrate the value of proteins and ribonucleoprotein complexes as blockers in low-temperature isothermal DNA amplification reactions for discrimination of nucleotide and epigenetic differences.
format	article
author	Toshitsugu Fujita Shoko Nagata Hodaka Fujii
author_facet	Toshitsugu Fujita Shoko Nagata Hodaka Fujii
author_sort	Toshitsugu Fujita
title	Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
title_short	Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
title_full	Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
title_fullStr	Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
title_full_unstemmed	Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
title_sort	protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c640dd47061a477aa32a90e8e90fa556
work_keys_str_mv	AT toshitsugufujita proteinorribonucleoproteinmediatedblockingofrecombinasepolymeraseamplificationenablesthediscriminationofnucleotideandepigeneticdifferencesbetweencellpopulations AT shokonagata proteinorribonucleoproteinmediatedblockingofrecombinasepolymeraseamplificationenablesthediscriminationofnucleotideandepigeneticdifferencesbetweencellpopulations AT hodakafujii proteinorribonucleoproteinmediatedblockingofrecombinasepolymeraseamplificationenablesthediscriminationofnucleotideandepigeneticdifferencesbetweencellpopulations
_version_	1718387719287078912

Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations

Ejemplares similares