Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient

Ahmed Majeed Al-Shammari,1 Mortadha A Alshami,2 Mahfoodha Abbas Umran,2 Asmaa Amer Almukhtar,3 Nahi Y Yaseen,1 Khansaa Raad,1 Ayman A Hussien1 1Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, 2Biotechnology Department, Collage of Scienc...

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Autores principales:	Al-Shammari AM, Alshami MA, Umran MA, Almukhtar AA, Yaseen NY, Raad K, Hussien AA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/c6485887dc124f4dabd955cf3cdf64ce
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spelling	oai:doaj.org-article:c6485887dc124f4dabd955cf3cdf64ce2021-12-02T03:15:40ZEstablishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient1179-1314https://doaj.org/article/c6485887dc124f4dabd955cf3cdf64ce2015-08-01T00:00:00Zhttp://www.dovepress.com/establishment-and-characterization-of-a-receptor-negative-hormone-nonr-peer-reviewed-article-BCTThttps://doaj.org/toc/1179-1314Ahmed Majeed Al-Shammari,1 Mortadha A Alshami,2 Mahfoodha Abbas Umran,2 Asmaa Amer Almukhtar,3 Nahi Y Yaseen,1 Khansaa Raad,1 Ayman A Hussien1 1Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, 2Biotechnology Department, Collage of Science, Baghdad University, 3Medical Genetics Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq Abstract: A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East. Keywords: ductal carcinoma, marker chromosomes, estrogen receptor, progesterone receptorAl-Shammari AMAlshami MAUmran MAAlmukhtar AAYaseen NYRaad KHussien AADove Medical PressarticleNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENBreast Cancer: Targets and Therapy, Vol 2015, Iss default, Pp 223-230 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Al-Shammari AM Alshami MA Umran MA Almukhtar AA Yaseen NY Raad K Hussien AA Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient
description	Ahmed Majeed Al-Shammari,1 Mortadha A Alshami,2 Mahfoodha Abbas Umran,2 Asmaa Amer Almukhtar,3 Nahi Y Yaseen,1 Khansaa Raad,1 Ayman A Hussien1 1Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, 2Biotechnology Department, Collage of Science, Baghdad University, 3Medical Genetics Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriya University, Baghdad, Iraq Abstract: A new breast cancer cell line (AMJ13) has been established from an Iraqi breast cancer patient. It is considered unique because it is the first for an Iraqi population, and is expected to be a useful tool in breast cancer research. The AMJ13 cell line was established from the primary tumor of a 70-year-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. The cells were morphologically characterized by light and scanning electron microscopy, and found to be elongated multipolar epithelial-like cells with a population doubling time of 22 hours. The anchorage-independent growth ability test showed that the cells were able to grow in semisolid agarose, confirming their transformed nature. Cytogenetic study of these cells showed chromosomal aberrations with many structural and numerical abnormalities, producing chromosomes of unknown origin called marker chromosomes. Immunocytochemistry showed that the estrogen receptor and the progesterone receptor were not expressed, and a weak positive result was found for HER2/neu gene expression. AMJ13 cells were positive for BRCA1 and BRCA2, as well as for vimentin. This cell line should be useful when testing new therapies for breast cancer in the Middle East. Keywords: ductal carcinoma, marker chromosomes, estrogen receptor, progesterone receptor
format	article
author	Al-Shammari AM Alshami MA Umran MA Almukhtar AA Yaseen NY Raad K Hussien AA
author_facet	Al-Shammari AM Alshami MA Umran MA Almukhtar AA Yaseen NY Raad K Hussien AA
author_sort	Al-Shammari AM
title	Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient
title_short	Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient
title_full	Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient
title_fullStr	Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient
title_full_unstemmed	Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient
title_sort	establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an iraqi patient
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/c6485887dc124f4dabd955cf3cdf64ce
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Establishment and characterization of a receptor-negative, hormone-nonresponsive breast cancer cell line from an Iraqi patient

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