Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1

Dishevelled (DVL) is the key component of Wnt signaling pathway that acts as a signaling hub. Here, authors study the conformational dynamics of DVL3 in vivo using their engineered FlAsH-based FRET biosensors and describe how Wnt activation opens DVL and facilitates Frizzled recruitment.

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Autores principales:	Jakub Harnoš, Maria Consuelo Alonso Cañizal, Miroslav Jurásek, Jitender Kumar, Cornelia Holler, Alexandra Schambony, Kateřina Hanáková, Ondřej Bernatík, Zbyněk Zdráhal, Kristína Gömöryová, Tomáš Gybeľ, Tomasz Witold Radaszkiewicz, Marek Kravec, Lukáš Trantírek, Jan Ryneš, Zankruti Dave, Ana Iris Fernández-Llamazares, Robert Vácha, Konstantinos Tripsianes, Carsten Hoffmann, Vítězslav Bryja
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/c656ec6d84244ea4ba47e39232d91995
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spelling	oai:doaj.org-article:c656ec6d84244ea4ba47e39232d919952021-12-02T14:38:40ZDishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 110.1038/s41467-019-09651-72041-1723https://doaj.org/article/c656ec6d84244ea4ba47e39232d919952019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09651-7https://doaj.org/toc/2041-1723Dishevelled (DVL) is the key component of Wnt signaling pathway that acts as a signaling hub. Here, authors study the conformational dynamics of DVL3 in vivo using their engineered FlAsH-based FRET biosensors and describe how Wnt activation opens DVL and facilitates Frizzled recruitment.Jakub HarnošMaria Consuelo Alonso CañizalMiroslav JurásekJitender KumarCornelia HollerAlexandra SchambonyKateřina HanákováOndřej BernatíkZbyněk ZdráhalKristína GömöryováTomáš GybeľTomasz Witold RadaszkiewiczMarek KravecLukáš TrantírekJan RynešZankruti DaveAna Iris Fernández-LlamazaresRobert VáchaKonstantinos TripsianesCarsten HoffmannVítězslav BryjaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-18 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jakub Harnoš Maria Consuelo Alonso Cañizal Miroslav Jurásek Jitender Kumar Cornelia Holler Alexandra Schambony Kateřina Hanáková Ondřej Bernatík Zbyněk Zdráhal Kristína Gömöryová Tomáš Gybeľ Tomasz Witold Radaszkiewicz Marek Kravec Lukáš Trantírek Jan Ryneš Zankruti Dave Ana Iris Fernández-Llamazares Robert Vácha Konstantinos Tripsianes Carsten Hoffmann Vítězslav Bryja Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
description	Dishevelled (DVL) is the key component of Wnt signaling pathway that acts as a signaling hub. Here, authors study the conformational dynamics of DVL3 in vivo using their engineered FlAsH-based FRET biosensors and describe how Wnt activation opens DVL and facilitates Frizzled recruitment.
format	article
author	Jakub Harnoš Maria Consuelo Alonso Cañizal Miroslav Jurásek Jitender Kumar Cornelia Holler Alexandra Schambony Kateřina Hanáková Ondřej Bernatík Zbyněk Zdráhal Kristína Gömöryová Tomáš Gybeľ Tomasz Witold Radaszkiewicz Marek Kravec Lukáš Trantírek Jan Ryneš Zankruti Dave Ana Iris Fernández-Llamazares Robert Vácha Konstantinos Tripsianes Carsten Hoffmann Vítězslav Bryja
author_facet	Jakub Harnoš Maria Consuelo Alonso Cañizal Miroslav Jurásek Jitender Kumar Cornelia Holler Alexandra Schambony Kateřina Hanáková Ondřej Bernatík Zbyněk Zdráhal Kristína Gömöryová Tomáš Gybeľ Tomasz Witold Radaszkiewicz Marek Kravec Lukáš Trantírek Jan Ryneš Zankruti Dave Ana Iris Fernández-Llamazares Robert Vácha Konstantinos Tripsianes Carsten Hoffmann Vítězslav Bryja
author_sort	Jakub Harnoš
title	Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
title_short	Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
title_full	Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
title_fullStr	Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
title_full_unstemmed	Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1
title_sort	dishevelled-3 conformation dynamics analyzed by fret-based biosensors reveals a key role of casein kinase 1
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/c656ec6d84244ea4ba47e39232d91995
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Dishevelled-3 conformation dynamics analyzed by FRET-based biosensors reveals a key role of casein kinase 1

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