Myosin 1b and F-actin are involved in the control of secretory granule biogenesis

Abstract Hormone secretion relies on secretory granules which store hormones in endocrine cells and release them upon cell stimulation. The molecular events leading to hormone sorting and secretory granule formation at the level of the TGN are still elusive. Our proteomic analysis of purified whole...

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Autores principales:	Charlène Delestre-Delacour, Ophélie Carmon, Fanny Laguerre, Catherine Estay-Ahumada, Maïté Courel, Salah Elias, Lydie Jeandel, Margarita Villar Rayo, Juan R. Peinado, Lucie Sengmanivong, Stéphane Gasman, Evelyne Coudrier, Youssef Anouar, Maité Montero-Hadjadje
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c658314d9f454de6b972e5ddac4717e6
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spelling	oai:doaj.org-article:c658314d9f454de6b972e5ddac4717e62021-12-02T11:52:23ZMyosin 1b and F-actin are involved in the control of secretory granule biogenesis10.1038/s41598-017-05617-12045-2322https://doaj.org/article/c658314d9f454de6b972e5ddac4717e62017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05617-1https://doaj.org/toc/2045-2322Abstract Hormone secretion relies on secretory granules which store hormones in endocrine cells and release them upon cell stimulation. The molecular events leading to hormone sorting and secretory granule formation at the level of the TGN are still elusive. Our proteomic analysis of purified whole secretory granules or secretory granule membranes uncovered their association with the actomyosin components myosin 1b, actin and the actin nucleation complex Arp2/3. We found that myosin 1b controls the formation of secretory granules and the associated regulated secretion in both neuroendocrine cells and chromogranin A-expressing COS7 cells used as a simplified model of induced secretion. We show that F-actin is also involved in secretory granule biogenesis and that myosin 1b cooperates with Arp2/3 to recruit F-actin to the Golgi region where secretory granules bud. These results provide the first evidence that components of the actomyosin complex promote the biogenesis of secretory granules and thereby regulate hormone sorting and secretion.Charlène Delestre-DelacourOphélie CarmonFanny LaguerreCatherine Estay-AhumadaMaïté CourelSalah EliasLydie JeandelMargarita Villar RayoJuan R. PeinadoLucie SengmanivongStéphane GasmanEvelyne CoudrierYoussef AnouarMaité Montero-HadjadjeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charlène Delestre-Delacour Ophélie Carmon Fanny Laguerre Catherine Estay-Ahumada Maïté Courel Salah Elias Lydie Jeandel Margarita Villar Rayo Juan R. Peinado Lucie Sengmanivong Stéphane Gasman Evelyne Coudrier Youssef Anouar Maité Montero-Hadjadje Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
description	Abstract Hormone secretion relies on secretory granules which store hormones in endocrine cells and release them upon cell stimulation. The molecular events leading to hormone sorting and secretory granule formation at the level of the TGN are still elusive. Our proteomic analysis of purified whole secretory granules or secretory granule membranes uncovered their association with the actomyosin components myosin 1b, actin and the actin nucleation complex Arp2/3. We found that myosin 1b controls the formation of secretory granules and the associated regulated secretion in both neuroendocrine cells and chromogranin A-expressing COS7 cells used as a simplified model of induced secretion. We show that F-actin is also involved in secretory granule biogenesis and that myosin 1b cooperates with Arp2/3 to recruit F-actin to the Golgi region where secretory granules bud. These results provide the first evidence that components of the actomyosin complex promote the biogenesis of secretory granules and thereby regulate hormone sorting and secretion.
format	article
author	Charlène Delestre-Delacour Ophélie Carmon Fanny Laguerre Catherine Estay-Ahumada Maïté Courel Salah Elias Lydie Jeandel Margarita Villar Rayo Juan R. Peinado Lucie Sengmanivong Stéphane Gasman Evelyne Coudrier Youssef Anouar Maité Montero-Hadjadje
author_facet	Charlène Delestre-Delacour Ophélie Carmon Fanny Laguerre Catherine Estay-Ahumada Maïté Courel Salah Elias Lydie Jeandel Margarita Villar Rayo Juan R. Peinado Lucie Sengmanivong Stéphane Gasman Evelyne Coudrier Youssef Anouar Maité Montero-Hadjadje
author_sort	Charlène Delestre-Delacour
title	Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
title_short	Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
title_full	Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
title_fullStr	Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
title_full_unstemmed	Myosin 1b and F-actin are involved in the control of secretory granule biogenesis
title_sort	myosin 1b and f-actin are involved in the control of secretory granule biogenesis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c658314d9f454de6b972e5ddac4717e6
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Myosin 1b and F-actin are involved in the control of secretory granule biogenesis

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