Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.

Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been long term interest in identifying spatial differences during biofilm development including clues that identify chemical heterogeneity. Laser ablation sample tr...

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Autores principales:	Aruni Chathurya Pulukkody, Yeni P Yung, Fabrizio Donnarumma, Kermit K Murray, Ross P Carlson, Luke Hanley
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c65835166d1b451386a3d0b7b51cd6f8
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spelling	oai:doaj.org-article:c65835166d1b451386a3d0b7b51cd6f82021-12-02T20:04:57ZSpatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.1932-620310.1371/journal.pone.0250911https://doaj.org/article/c65835166d1b451386a3d0b7b51cd6f82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0250911https://doaj.org/toc/1932-6203Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been long term interest in identifying spatial differences during biofilm development including clues that identify chemical heterogeneity. Laser ablation sample transfer (LAST) allows site-specific sampling combined with label free proteomics to distinguish radially and axially resolved proteomes for Pseudomonas aeruginosa biofilms. Specifically, differential protein abundances on oxic vs. anoxic regions of a biofilm were observed by combining LAST with bottom up proteomics. This study reveals a more active metabolism in the anoxic region of the biofilm with respect to the oxic region for this clinical strain of P. aeruginosa, despite this organism being considered an aerobe by nature. Protein abundance data related to cellular acclimations to chemical gradients include identification of glucose catabolizing proteins, high abundance of proteins from arginine and polyamine metabolism, and proteins that could also support virulence and environmental stress mediation in the anoxic region. Finally, the LAST methodology requires only a few mm2 of biofilm area to identify hundreds of proteins.Aruni Chathurya PulukkodyYeni P YungFabrizio DonnarummaKermit K MurrayRoss P CarlsonLuke HanleyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0250911 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aruni Chathurya Pulukkody Yeni P Yung Fabrizio Donnarumma Kermit K Murray Ross P Carlson Luke Hanley Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
description	Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been long term interest in identifying spatial differences during biofilm development including clues that identify chemical heterogeneity. Laser ablation sample transfer (LAST) allows site-specific sampling combined with label free proteomics to distinguish radially and axially resolved proteomes for Pseudomonas aeruginosa biofilms. Specifically, differential protein abundances on oxic vs. anoxic regions of a biofilm were observed by combining LAST with bottom up proteomics. This study reveals a more active metabolism in the anoxic region of the biofilm with respect to the oxic region for this clinical strain of P. aeruginosa, despite this organism being considered an aerobe by nature. Protein abundance data related to cellular acclimations to chemical gradients include identification of glucose catabolizing proteins, high abundance of proteins from arginine and polyamine metabolism, and proteins that could also support virulence and environmental stress mediation in the anoxic region. Finally, the LAST methodology requires only a few mm2 of biofilm area to identify hundreds of proteins.
format	article
author	Aruni Chathurya Pulukkody Yeni P Yung Fabrizio Donnarumma Kermit K Murray Ross P Carlson Luke Hanley
author_facet	Aruni Chathurya Pulukkody Yeni P Yung Fabrizio Donnarumma Kermit K Murray Ross P Carlson Luke Hanley
author_sort	Aruni Chathurya Pulukkody
title	Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_short	Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_full	Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_fullStr	Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_full_unstemmed	Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_sort	spatially resolved analysis of pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/c65835166d1b451386a3d0b7b51cd6f8
work_keys_str_mv	AT arunichathuryapulukkody spatiallyresolvedanalysisofpseudomonasaeruginosabiofilmproteomesmeasuredbylaserablationsampletransfer AT yenipyung spatiallyresolvedanalysisofpseudomonasaeruginosabiofilmproteomesmeasuredbylaserablationsampletransfer AT fabriziodonnarumma spatiallyresolvedanalysisofpseudomonasaeruginosabiofilmproteomesmeasuredbylaserablationsampletransfer AT kermitkmurray spatiallyresolvedanalysisofpseudomonasaeruginosabiofilmproteomesmeasuredbylaserablationsampletransfer AT rosspcarlson spatiallyresolvedanalysisofpseudomonasaeruginosabiofilmproteomesmeasuredbylaserablationsampletransfer AT lukehanley spatiallyresolvedanalysisofpseudomonasaeruginosabiofilmproteomesmeasuredbylaserablationsampletransfer
_version_	1718375567636561920

Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.

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