Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.

Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been long term interest in identifying spatial differences during biofilm development including clues that identify chemical heterogeneity. Laser ablation sample tr...

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Autores principales: Aruni Chathurya Pulukkody, Yeni P Yung, Fabrizio Donnarumma, Kermit K Murray, Ross P Carlson, Luke Hanley
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Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/c65835166d1b451386a3d0b7b51cd6f8
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spelling oai:doaj.org-article:c65835166d1b451386a3d0b7b51cd6f82021-12-02T20:04:57ZSpatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.1932-620310.1371/journal.pone.0250911https://doaj.org/article/c65835166d1b451386a3d0b7b51cd6f82021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0250911https://doaj.org/toc/1932-6203Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been long term interest in identifying spatial differences during biofilm development including clues that identify chemical heterogeneity. Laser ablation sample transfer (LAST) allows site-specific sampling combined with label free proteomics to distinguish radially and axially resolved proteomes for Pseudomonas aeruginosa biofilms. Specifically, differential protein abundances on oxic vs. anoxic regions of a biofilm were observed by combining LAST with bottom up proteomics. This study reveals a more active metabolism in the anoxic region of the biofilm with respect to the oxic region for this clinical strain of P. aeruginosa, despite this organism being considered an aerobe by nature. Protein abundance data related to cellular acclimations to chemical gradients include identification of glucose catabolizing proteins, high abundance of proteins from arginine and polyamine metabolism, and proteins that could also support virulence and environmental stress mediation in the anoxic region. Finally, the LAST methodology requires only a few mm2 of biofilm area to identify hundreds of proteins.Aruni Chathurya PulukkodyYeni P YungFabrizio DonnarummaKermit K MurrayRoss P CarlsonLuke HanleyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0250911 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Aruni Chathurya Pulukkody
Yeni P Yung
Fabrizio Donnarumma
Kermit K Murray
Ross P Carlson
Luke Hanley
Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
description Heterogeneity in the distribution of nutrients and oxygen gradients during biofilm growth gives rise to changes in phenotype. There has been long term interest in identifying spatial differences during biofilm development including clues that identify chemical heterogeneity. Laser ablation sample transfer (LAST) allows site-specific sampling combined with label free proteomics to distinguish radially and axially resolved proteomes for Pseudomonas aeruginosa biofilms. Specifically, differential protein abundances on oxic vs. anoxic regions of a biofilm were observed by combining LAST with bottom up proteomics. This study reveals a more active metabolism in the anoxic region of the biofilm with respect to the oxic region for this clinical strain of P. aeruginosa, despite this organism being considered an aerobe by nature. Protein abundance data related to cellular acclimations to chemical gradients include identification of glucose catabolizing proteins, high abundance of proteins from arginine and polyamine metabolism, and proteins that could also support virulence and environmental stress mediation in the anoxic region. Finally, the LAST methodology requires only a few mm2 of biofilm area to identify hundreds of proteins.
format article
author Aruni Chathurya Pulukkody
Yeni P Yung
Fabrizio Donnarumma
Kermit K Murray
Ross P Carlson
Luke Hanley
author_facet Aruni Chathurya Pulukkody
Yeni P Yung
Fabrizio Donnarumma
Kermit K Murray
Ross P Carlson
Luke Hanley
author_sort Aruni Chathurya Pulukkody
title Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_short Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_full Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_fullStr Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_full_unstemmed Spatially resolved analysis of Pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
title_sort spatially resolved analysis of pseudomonas aeruginosa biofilm proteomes measured by laser ablation sample transfer.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/c65835166d1b451386a3d0b7b51cd6f8
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