Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>

The intake of isoflavones is presumed to be associated with health benefits in humans, but also potential adverse effects of isoflavones are controversially discussed. Isoflavones can be metabolized by gut bacteria leading to modulation of the bioactivity, such as estrogenic effects. Especially bact...

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Autores principales:	Sebastian Tobias Soukup, Dominic Alexander Stoll, Nicolas Danylec, Alena Schoepf, Sabine Emma Kulling, Melanie Huch
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Eggerthellaceae</i> <i>Coriobacteriaceae</i> anaerobic isoflavones daidzein genistein Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/c658cb858d3f4778aec067e5556e5029
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spelling	oai:doaj.org-article:c658cb858d3f4778aec067e5556e50292021-11-25T17:35:05ZMetabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>10.3390/foods101127412304-8158https://doaj.org/article/c658cb858d3f4778aec067e5556e50292021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2741https://doaj.org/toc/2304-8158The intake of isoflavones is presumed to be associated with health benefits in humans, but also potential adverse effects of isoflavones are controversially discussed. Isoflavones can be metabolized by gut bacteria leading to modulation of the bioactivity, such as estrogenic effects. Especially bacterial strains of the <i>Eggerthellaceae,</i> a well-known bacterial family of the human gut microbiota, are able to convert the isoflavone daidzein into equol. In addition, metabolization of genistein is also described for strains of the <i>Eggerthellaceae</i>. The aim of this study was to identify and investigate gut bacterial strains of the family <i>Eggerthellaceae</i> as well as the narrowly related family <i>Coriobacteriaceae</i> which are able to metabolize daidzein and genistein. This study provides a comprehensive, polyphasic approach comprising in silico analysis of the equol gene cluster, detection of genes associated with the daidzein, and genistein metabolism via PCR and fermentation of these isoflavones. The in silico search for protein sequences that are associated with daidzein metabolism identified sequences with high similarity values in already well-known equol-producing strains. Furthermore, protein sequences that are presumed to be associated with daidzein and genistein metabolism were detected in the two type strains ‘<i>Hugonella massiliensis</i>’ and <i>Senegalimassilia faecalis</i> which were not yet described to metabolize these isoflavones. An alignment of these protein sequences showed that the equol gene cluster is highly conserved. In addition, PCR amplification supported the presence of genes associated with daidzein and genistein metabolism. Furthermore, the metabolism of daidzein and genistein was investigated in fermentations of pure bacterial cultures under strictly anaerobic conditions and proofed the metabolism of daidzein and genistein by the strains ‘<i>Hugonella massiliensis</i>’ DSM 101782<sup>T</sup> and <i>Senegalimassilia faecalis</i> KGMB04484<sup>T</sup>.Sebastian Tobias SoukupDominic Alexander StollNicolas DanylecAlena SchoepfSabine Emma KullingMelanie HuchMDPI AGarticle<i>Eggerthellaceae</i><i>Coriobacteriaceae</i>anaerobicisoflavonesdaidzeingenisteinChemical technologyTP1-1185ENFoods, Vol 10, Iss 2741, p 2741 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Eggerthellaceae</i> <i>Coriobacteriaceae</i> anaerobic isoflavones daidzein genistein Chemical technology TP1-1185
spellingShingle	<i>Eggerthellaceae</i> <i>Coriobacteriaceae</i> anaerobic isoflavones daidzein genistein Chemical technology TP1-1185 Sebastian Tobias Soukup Dominic Alexander Stoll Nicolas Danylec Alena Schoepf Sabine Emma Kulling Melanie Huch Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>
description	The intake of isoflavones is presumed to be associated with health benefits in humans, but also potential adverse effects of isoflavones are controversially discussed. Isoflavones can be metabolized by gut bacteria leading to modulation of the bioactivity, such as estrogenic effects. Especially bacterial strains of the <i>Eggerthellaceae,</i> a well-known bacterial family of the human gut microbiota, are able to convert the isoflavone daidzein into equol. In addition, metabolization of genistein is also described for strains of the <i>Eggerthellaceae</i>. The aim of this study was to identify and investigate gut bacterial strains of the family <i>Eggerthellaceae</i> as well as the narrowly related family <i>Coriobacteriaceae</i> which are able to metabolize daidzein and genistein. This study provides a comprehensive, polyphasic approach comprising in silico analysis of the equol gene cluster, detection of genes associated with the daidzein, and genistein metabolism via PCR and fermentation of these isoflavones. The in silico search for protein sequences that are associated with daidzein metabolism identified sequences with high similarity values in already well-known equol-producing strains. Furthermore, protein sequences that are presumed to be associated with daidzein and genistein metabolism were detected in the two type strains ‘<i>Hugonella massiliensis</i>’ and <i>Senegalimassilia faecalis</i> which were not yet described to metabolize these isoflavones. An alignment of these protein sequences showed that the equol gene cluster is highly conserved. In addition, PCR amplification supported the presence of genes associated with daidzein and genistein metabolism. Furthermore, the metabolism of daidzein and genistein was investigated in fermentations of pure bacterial cultures under strictly anaerobic conditions and proofed the metabolism of daidzein and genistein by the strains ‘<i>Hugonella massiliensis</i>’ DSM 101782<sup>T</sup> and <i>Senegalimassilia faecalis</i> KGMB04484<sup>T</sup>.
format	article
author	Sebastian Tobias Soukup Dominic Alexander Stoll Nicolas Danylec Alena Schoepf Sabine Emma Kulling Melanie Huch
author_facet	Sebastian Tobias Soukup Dominic Alexander Stoll Nicolas Danylec Alena Schoepf Sabine Emma Kulling Melanie Huch
author_sort	Sebastian Tobias Soukup
title	Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>
title_short	Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>
title_full	Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>
title_fullStr	Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>
title_full_unstemmed	Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>
title_sort	metabolism of daidzein and genistein by gut bacteria of the class <i>coriobacteriia</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c658cb858d3f4778aec067e5556e5029
work_keys_str_mv	AT sebastiantobiassoukup metabolismofdaidzeinandgenisteinbygutbacteriaoftheclassicoriobacteriiai AT dominicalexanderstoll metabolismofdaidzeinandgenisteinbygutbacteriaoftheclassicoriobacteriiai AT nicolasdanylec metabolismofdaidzeinandgenisteinbygutbacteriaoftheclassicoriobacteriiai AT alenaschoepf metabolismofdaidzeinandgenisteinbygutbacteriaoftheclassicoriobacteriiai AT sabineemmakulling metabolismofdaidzeinandgenisteinbygutbacteriaoftheclassicoriobacteriiai AT melaniehuch metabolismofdaidzeinandgenisteinbygutbacteriaoftheclassicoriobacteriiai
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Metabolism of Daidzein and Genistein by Gut Bacteria of the Class <i>Coriobacteriia</i>

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