Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds

In vivo tissues, including the cardiac, skeletal muscles, tendon and ligaments display characteristic alignment property which is important for their mechanical property and functionality. Mimicking this alignment property is critical to the realization of physiologically relevant cell sheets for po...

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Autores principales:	Kennedy Omondi Okeyo, Yoshikiyo Kibe, Taiji Adachi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Regenerative medicine Cell sheets Cell alignment Cell adhesion Scaffold microstructure Geometry sensing Materials of engineering and construction. Mechanics of materials TA401-492
Acceso en línea:	https://doaj.org/article/c65ddb7ed92340678096c0ad74a59058
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spelling	oai:doaj.org-article:c65ddb7ed92340678096c0ad74a590582021-12-04T04:35:40ZControlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds2590-049810.1016/j.mtadv.2021.100194https://doaj.org/article/c65ddb7ed92340678096c0ad74a590582021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590049821000643https://doaj.org/toc/2590-0498In vivo tissues, including the cardiac, skeletal muscles, tendon and ligaments display characteristic alignment property which is important for their mechanical property and functionality. Mimicking this alignment property is critical to the realization of physiologically relevant cell sheets for potential application in the regeneration of aligned in vivo tissues. In this study, we aimed to achieve fabrication of aligned cell sheets by harnessing the ability of cells to sense and respond to geometrical cues in their adhesion microenvironment. We demonstrate that macroscale cell alignment in cell sheets formed by C2C12 cells, a mouse myoblast cell line, on adhesion limiting microstructured mesh scaffolds depends on the shape of the scaffold microstructure. Specifically, while square meshes produced cell sheets with random orientation, diamond meshes yielded anisotropic cell sheets with cells aligned uniaxially along the major axis of the diamond shape. Moreover, alignment intensity was found to increase concomitantly with the acuteness of the diamond shape, illustrating alignment dependency on mesh shape anisotropy. Remarkably, myotubes derived from aligned C2C12 cells also displayed a similar alignment trend, demonstrating the robustness of our approach. Taken together, the present study demonstrates the potential to control macroscale cell alignment in self-organized cell sheets by tuning the shape of the scaffold microstructure. Thus, insights from this study could be relevant to the design of instructive scaffolds for fabricating aligned cell sheets for potential application not only in regenerative medicine, but also in developing muscle constructs for toxicity assays.Kennedy Omondi OkeyoYoshikiyo KibeTaiji AdachiElsevierarticleRegenerative medicineCell sheetsCell alignmentCell adhesionScaffold microstructureGeometry sensingMaterials of engineering and construction. Mechanics of materialsTA401-492ENMaterials Today Advances, Vol 12, Iss , Pp 100194- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Regenerative medicine Cell sheets Cell alignment Cell adhesion Scaffold microstructure Geometry sensing Materials of engineering and construction. Mechanics of materials TA401-492
spellingShingle	Regenerative medicine Cell sheets Cell alignment Cell adhesion Scaffold microstructure Geometry sensing Materials of engineering and construction. Mechanics of materials TA401-492 Kennedy Omondi Okeyo Yoshikiyo Kibe Taiji Adachi Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
description	In vivo tissues, including the cardiac, skeletal muscles, tendon and ligaments display characteristic alignment property which is important for their mechanical property and functionality. Mimicking this alignment property is critical to the realization of physiologically relevant cell sheets for potential application in the regeneration of aligned in vivo tissues. In this study, we aimed to achieve fabrication of aligned cell sheets by harnessing the ability of cells to sense and respond to geometrical cues in their adhesion microenvironment. We demonstrate that macroscale cell alignment in cell sheets formed by C2C12 cells, a mouse myoblast cell line, on adhesion limiting microstructured mesh scaffolds depends on the shape of the scaffold microstructure. Specifically, while square meshes produced cell sheets with random orientation, diamond meshes yielded anisotropic cell sheets with cells aligned uniaxially along the major axis of the diamond shape. Moreover, alignment intensity was found to increase concomitantly with the acuteness of the diamond shape, illustrating alignment dependency on mesh shape anisotropy. Remarkably, myotubes derived from aligned C2C12 cells also displayed a similar alignment trend, demonstrating the robustness of our approach. Taken together, the present study demonstrates the potential to control macroscale cell alignment in self-organized cell sheets by tuning the shape of the scaffold microstructure. Thus, insights from this study could be relevant to the design of instructive scaffolds for fabricating aligned cell sheets for potential application not only in regenerative medicine, but also in developing muscle constructs for toxicity assays.
format	article
author	Kennedy Omondi Okeyo Yoshikiyo Kibe Taiji Adachi
author_facet	Kennedy Omondi Okeyo Yoshikiyo Kibe Taiji Adachi
author_sort	Kennedy Omondi Okeyo
title	Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
title_short	Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
title_full	Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
title_fullStr	Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
title_full_unstemmed	Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
title_sort	controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c65ddb7ed92340678096c0ad74a59058
work_keys_str_mv	AT kennedyomondiokeyo controllingmacroscalecellalignmentinselforganizedcellsheetsbytuningthemicrostructureofadhesionlimitingmicromeshscaffolds AT yoshikiyokibe controllingmacroscalecellalignmentinselforganizedcellsheetsbytuningthemicrostructureofadhesionlimitingmicromeshscaffolds AT taijiadachi controllingmacroscalecellalignmentinselforganizedcellsheetsbytuningthemicrostructureofadhesionlimitingmicromeshscaffolds
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Controlling macroscale cell alignment in self-organized cell sheets by tuning the microstructure of adhesion-limiting micromesh scaffolds

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