Magnetic bead purification of enveloped alphavirus and flavivirus

Virus production is an essential part of virology. The quality of virus preparation, in terms of purification and yield, can affect the outcome of experiments. Purified virus is critical for assays, as contamination from cell culture supernatants may affect results. Purified virus is also needed for...

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Autores principales:	Bianca Galasso, Shreya Sharma, Barbara Knollmann-Ritschel, Anuj Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Magnetic capture, concentration, and conservation of flaviviruses and alphaviruses Science Q
Acceso en línea:	https://doaj.org/article/c66cad251d3c4cf79da051c6dee9af2a
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spelling	oai:doaj.org-article:c66cad251d3c4cf79da051c6dee9af2a2021-11-06T04:29:25ZMagnetic bead purification of enveloped alphavirus and flavivirus2215-016110.1016/j.mex.2021.101549https://doaj.org/article/c66cad251d3c4cf79da051c6dee9af2a2021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2215016121003411https://doaj.org/toc/2215-0161Virus production is an essential part of virology. The quality of virus preparation, in terms of purification and yield, can affect the outcome of experiments. Purified virus is critical for assays, as contamination from cell culture supernatants may affect results. Purified virus is also needed for vaccine preparations. The traditional process of gradient purification of virus is time consuming, with multiple steps spanning over days, and produces significant amounts of biohazard waste. Developing a more efficient alternative that provides a purified virus product is highly desirable. In this study, we performed magnetic bead purification of Zika virus (ZIKV) and Mayaro virus (MAYV) with the Mag4C LV kit from OZ Biosciences. Currently, this kit is only indicated for use with lentiviruses and retroviruses. We found that we were able to successfully concentrate and purify both ZIKV and MAYV using the kit. Therefore, we concluded that the Mag4C LV kit provides a quick and simple alternative to traditional virus purification methods. • Our protocol is customized by using an alphavirus (MAYV) and flavivirus (ZIKV). This method has not been previously used for these viruses.Bianca GalassoShreya SharmaBarbara Knollmann-RitschelAnuj SharmaElsevierarticleMagnetic capture, concentration, and conservation of flaviviruses and alphavirusesScienceQENMethodsX, Vol 8, Iss , Pp 101549- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Magnetic capture, concentration, and conservation of flaviviruses and alphaviruses Science Q
spellingShingle	Magnetic capture, concentration, and conservation of flaviviruses and alphaviruses Science Q Bianca Galasso Shreya Sharma Barbara Knollmann-Ritschel Anuj Sharma Magnetic bead purification of enveloped alphavirus and flavivirus
description	Virus production is an essential part of virology. The quality of virus preparation, in terms of purification and yield, can affect the outcome of experiments. Purified virus is critical for assays, as contamination from cell culture supernatants may affect results. Purified virus is also needed for vaccine preparations. The traditional process of gradient purification of virus is time consuming, with multiple steps spanning over days, and produces significant amounts of biohazard waste. Developing a more efficient alternative that provides a purified virus product is highly desirable. In this study, we performed magnetic bead purification of Zika virus (ZIKV) and Mayaro virus (MAYV) with the Mag4C LV kit from OZ Biosciences. Currently, this kit is only indicated for use with lentiviruses and retroviruses. We found that we were able to successfully concentrate and purify both ZIKV and MAYV using the kit. Therefore, we concluded that the Mag4C LV kit provides a quick and simple alternative to traditional virus purification methods. • Our protocol is customized by using an alphavirus (MAYV) and flavivirus (ZIKV). This method has not been previously used for these viruses.
format	article
author	Bianca Galasso Shreya Sharma Barbara Knollmann-Ritschel Anuj Sharma
author_facet	Bianca Galasso Shreya Sharma Barbara Knollmann-Ritschel Anuj Sharma
author_sort	Bianca Galasso
title	Magnetic bead purification of enveloped alphavirus and flavivirus
title_short	Magnetic bead purification of enveloped alphavirus and flavivirus
title_full	Magnetic bead purification of enveloped alphavirus and flavivirus
title_fullStr	Magnetic bead purification of enveloped alphavirus and flavivirus
title_full_unstemmed	Magnetic bead purification of enveloped alphavirus and flavivirus
title_sort	magnetic bead purification of enveloped alphavirus and flavivirus
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c66cad251d3c4cf79da051c6dee9af2a
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Magnetic bead purification of enveloped alphavirus and flavivirus

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