Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy

Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical compositi...

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Autores principales:	Tamilla Mirzayeva, Jana Čopíková, František Kvasnička, Roman Bleha, Andriy Synytsya
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hyaluronic acid determination food supplements Fourier-transform infrared spectroscopy Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/c6734998919c4a32be5b3050346cada7
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spelling	oai:doaj.org-article:c6734998919c4a32be5b3050346cada72021-11-25T18:49:19ZScreening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy10.3390/polym132240022073-4360https://doaj.org/article/c6734998919c4a32be5b3050346cada72021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/4002https://doaj.org/toc/2073-4360Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical composition of three chosen dietary supplements (powder, tablets and capsules) containing hyaluronic acid was carried out using Fourier-transform infrared spectroscopy. Because of the low amount of analyte in all these samples, it was isolated or concentrated prior to the analysis using a suitable sequential fractionation protocol. Individual isolation procedures were established for each sample based on their declared composition. Firstly, the major components such as collagen or vitamins were removed to obtain polysaccharide fractions by the enzymatic treatment and/or washing out with the appropriate solvents. In some cases, the water insoluble part was removed from the rest dissolved in water. Then, hyaluronic acid was precipitated with copper(II) cations and thus separated from the other polysaccharides. Finally, the analyte was identified in the enriched fractions by the characteristic vibrational bands. The amount of hyaluronic acid in the purified fractions was determined in three ways: gravimetrically, spectrophotometrically, and using isotachophoresis. The combination of the appropriate preparative and analytical steps led to the successful evaluation of chemical composition, finding and quantification of hyaluronic acid in all the studied samples.Tamilla MirzayevaJana ČopíkováFrantišek KvasničkaRoman BlehaAndriy SynytsyaMDPI AGarticlehyaluronic aciddeterminationfood supplementsFourier-transform infrared spectroscopyOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 4002, p 4002 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hyaluronic acid determination food supplements Fourier-transform infrared spectroscopy Organic chemistry QD241-441
spellingShingle	hyaluronic acid determination food supplements Fourier-transform infrared spectroscopy Organic chemistry QD241-441 Tamilla Mirzayeva Jana Čopíková František Kvasnička Roman Bleha Andriy Synytsya Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
description	Hyaluronic acid, together with collagen, vitamins or plant extracts, is a part of many cosmetic and food preparations. For example, this polysaccharide is used in formulation of many food supplements due to its protective effects on human health. In this work, the screening of the chemical composition of three chosen dietary supplements (powder, tablets and capsules) containing hyaluronic acid was carried out using Fourier-transform infrared spectroscopy. Because of the low amount of analyte in all these samples, it was isolated or concentrated prior to the analysis using a suitable sequential fractionation protocol. Individual isolation procedures were established for each sample based on their declared composition. Firstly, the major components such as collagen or vitamins were removed to obtain polysaccharide fractions by the enzymatic treatment and/or washing out with the appropriate solvents. In some cases, the water insoluble part was removed from the rest dissolved in water. Then, hyaluronic acid was precipitated with copper(II) cations and thus separated from the other polysaccharides. Finally, the analyte was identified in the enriched fractions by the characteristic vibrational bands. The amount of hyaluronic acid in the purified fractions was determined in three ways: gravimetrically, spectrophotometrically, and using isotachophoresis. The combination of the appropriate preparative and analytical steps led to the successful evaluation of chemical composition, finding and quantification of hyaluronic acid in all the studied samples.
format	article
author	Tamilla Mirzayeva Jana Čopíková František Kvasnička Roman Bleha Andriy Synytsya
author_facet	Tamilla Mirzayeva Jana Čopíková František Kvasnička Roman Bleha Andriy Synytsya
author_sort	Tamilla Mirzayeva
title	Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_short	Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_full	Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_fullStr	Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_full_unstemmed	Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy
title_sort	screening of the chemical composition and identification of hyaluronic acid in food supplements by fractionation and fourier-transform infrared spectroscopy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c6734998919c4a32be5b3050346cada7
work_keys_str_mv	AT tamillamirzayeva screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy AT janacopikova screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy AT frantisekkvasnicka screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy AT romanbleha screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy AT andriysynytsya screeningofthechemicalcompositionandidentificationofhyaluronicacidinfoodsupplementsbyfractionationandfouriertransforminfraredspectroscopy
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Screening of the Chemical Composition and Identification of Hyaluronic Acid in Food Supplements by Fractionation and Fourier-Transform Infrared Spectroscopy

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