Gene Expression Profiling and Clinicopathological Importance of Fer1L4 and DANCR Long Non Coding RNAs in Patients with Head and Neck Squamous Cell Carcinoma
Introduction: Head and Neck Squamous Cell Carcinoma (HNSCC) entails a heterogeneous group of tumours that emerge from the interaction between molecular changes and environmental factors. Dysregulated long noncoding RNAs (LncRNAs) play a major part in tumourigenesis and could be used as cancer b...
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| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
JCDR Research and Publications Private Limited
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/c675af1aff8c4905a59b468c2fe88f6f |
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| Sumario: | Introduction: Head and Neck Squamous Cell Carcinoma (HNSCC)
entails a heterogeneous group of tumours that emerge from
the interaction between molecular changes and environmental
factors. Dysregulated long noncoding RNAs (LncRNAs) play
a major part in tumourigenesis and could be used as cancer
biomarkers and therapeutic aims.
Aim: To evaluate the expression of two lncRNAs named Fer-1
Like Family Member 4 (Fer1L4) and differentiation antagonising
non protein-coding RNA (DANCR) in tumoural tissue of HNSCCs
patients in comparison to Adjacent Non cancerous Tissues
(ANCTs) to appraise their diagnostic power and the relationship
with clinicopathological parameters.
Materials and Methods: The present case-control study was
designed, in which fresh frozen cancerous tissues and ANCTs
were taken from 50 sporadic HNSCC patients who were
attended in Imam Khomeini and Amir Alam Hospitals (Tehran,
Iran) from from January to December 2019. Real-time PCR
was utilised for expression profiling of Fer1L4 and DANCR. By
employing GraphPad Prism 8.0 GraphPad Software, Inc., San
Diego, CA, the real-time quantitative PCR experiments(2-..Ct)
method and the Mann-Whitney test were exerted to analyse the
obtained data. The Receiver Operating Characteristic (ROC)
curve analysis was employed for figuring out the discrimination
potential of two selected lncRNAs between the subject tumour
and ANCT.
Results: The expression of Fer1L4 was significantly down-regulated
in tumoural tissues by analogy to ANCTs (p-value <0.0001) and
statistically significant associations were found between the
stage and grade status of the tumour with the relative expression
of this lncRNA (p-value=0.008 and p-value=0.002 for stage and
grade, respectively). The findings in this study indicated that the
expression of DANCR was not statistically significant different in
different tumoural tissues compared with ANCTs (p-value=0.46).
ROC curve unraveled that the Fer1L4 had good diagnostic power
Area Under Curve (AUC) 0.9252; p-value <0.0001. The expression
of DANCR and Fer1L4 was significantly, respectively, higher and
lower in samples with lymph node invasion and metastasis than
that of the counterpart group. Concerning Human Papillomavirus
(HPV) as an important exogenous factor for the development of
HNSCC, DANCR and Fer1L4 were over-expressed and underexpressed, respectively in the HPV+group in comparison to HPV-.
Conclusion: This work represented that Fer1L4 could be used
as a novel diagnostic biomarker for HNSCC. In addition, the
statistically significant difference in the expression of Fer1L4
and DANCR in metastatic tumours demonstrated that these two
lncRNAs are promising targets for therapeutic purposes. |
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