Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells

Pal Patel, Krupa Kansara, Ragini Singh, Ritesh K Shukla, Sanjay Singh, Alok Dhawan, Ashutosh Kumar Division of Biological and Life Sciences (Formerly Institute of Life Sciences), School of Arts and Sciences, Ahmedabad University, Ahmedabad, Gujarat, India Abstract: Overproduction of free radicals...

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Autores principales:	Patel P, Kansara K, Singh R, Shukla RK, Singh S, Dhawan A, Kumar A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Nanoceria Reactive Oxygen Species Inflammation Free radicals Antioxidant. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c676a65906bc4e49869e99d4a0bab510
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spelling	oai:doaj.org-article:c676a65906bc4e49869e99d4a0bab5102021-12-02T01:27:00ZCellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells1178-2013https://doaj.org/article/c676a65906bc4e49869e99d4a0bab5102018-03-01T00:00:00Zhttps://www.dovepress.com/cellular-internalization-and-antioxidant-activity-of-cerium-oxide-nano-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Pal Patel, Krupa Kansara, Ragini Singh, Ritesh K Shukla, Sanjay Singh, Alok Dhawan, Ashutosh Kumar Division of Biological and Life Sciences (Formerly Institute of Life Sciences), School of Arts and Sciences, Ahmedabad University, Ahmedabad, Gujarat, India Abstract: Overproduction of free radicals contributes to oxidative stress and inflammation leading to various disease conditions. Cerium oxide nanoparticles (nanoceria) have been shown to scavenge free radicals and have the potential for being used as a therapeutic agent in disease conditions. Therefore, in the present study, human monocytic leukemia cells (THP-1) were used as a model to evaluate the uptake and free radical scavenging activity of nanoceria. Our data showed a significant (P<0.05) increase in the internalization of nanoceria in a concentration-dependent (10–100 µg/mL) manner in THP-1 cells. Although no cytotoxicity was observed at these concentrations, nanoceria significantly (P<0.05) reduced the amount of reactive oxygen species. This was evident by a significant (P<0.05) decrease in the 2,7-dichlorofluorescein diacetate fluorescence observed in flow cytometry and fluorescence microscopy. The present study shows that nanoceria have therapeutic potential in diseases such as cancer. Keywords: nanoceria, reactive oxygen species, inflammation, free radicals, antioxidantPatel PKansara KSingh RShukla RKSingh SDhawan AKumar ADove Medical PressarticleNanoceriaReactive Oxygen SpeciesInflammationFree radicalsAntioxidant.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 39-41 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nanoceria Reactive Oxygen Species Inflammation Free radicals Antioxidant. Medicine (General) R5-920
spellingShingle	Nanoceria Reactive Oxygen Species Inflammation Free radicals Antioxidant. Medicine (General) R5-920 Patel P Kansara K Singh R Shukla RK Singh S Dhawan A Kumar A Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
description	Pal Patel, Krupa Kansara, Ragini Singh, Ritesh K Shukla, Sanjay Singh, Alok Dhawan, Ashutosh Kumar Division of Biological and Life Sciences (Formerly Institute of Life Sciences), School of Arts and Sciences, Ahmedabad University, Ahmedabad, Gujarat, India Abstract: Overproduction of free radicals contributes to oxidative stress and inflammation leading to various disease conditions. Cerium oxide nanoparticles (nanoceria) have been shown to scavenge free radicals and have the potential for being used as a therapeutic agent in disease conditions. Therefore, in the present study, human monocytic leukemia cells (THP-1) were used as a model to evaluate the uptake and free radical scavenging activity of nanoceria. Our data showed a significant (P<0.05) increase in the internalization of nanoceria in a concentration-dependent (10–100 µg/mL) manner in THP-1 cells. Although no cytotoxicity was observed at these concentrations, nanoceria significantly (P<0.05) reduced the amount of reactive oxygen species. This was evident by a significant (P<0.05) decrease in the 2,7-dichlorofluorescein diacetate fluorescence observed in flow cytometry and fluorescence microscopy. The present study shows that nanoceria have therapeutic potential in diseases such as cancer. Keywords: nanoceria, reactive oxygen species, inflammation, free radicals, antioxidant
format	article
author	Patel P Kansara K Singh R Shukla RK Singh S Dhawan A Kumar A
author_facet	Patel P Kansara K Singh R Shukla RK Singh S Dhawan A Kumar A
author_sort	Patel P
title	Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
title_short	Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
title_full	Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
title_fullStr	Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
title_full_unstemmed	Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
title_sort	cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/c676a65906bc4e49869e99d4a0bab510
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Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells

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