JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns

Abstract As the excitation–contraction coupling is inseparable during voluntary exercise, the relative contribution of the mechanical and neural input on hypertrophy-related molecular signalling is still poorly understood. Herein, we use a rat in-vivo strength exercise model with an electrically-ind...

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Autores principales:	Einar Eftestøl, Martino V. Franchi, Stephanie Kasper, Martin Flück
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c682d1a9e6ed475f8842813954f2a609
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spelling	oai:doaj.org-article:c682d1a9e6ed475f8842813954f2a6092021-12-02T16:27:50ZJNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns10.1038/s41598-021-94930-x2045-2322https://doaj.org/article/c682d1a9e6ed475f8842813954f2a6092021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94930-xhttps://doaj.org/toc/2045-2322Abstract As the excitation–contraction coupling is inseparable during voluntary exercise, the relative contribution of the mechanical and neural input on hypertrophy-related molecular signalling is still poorly understood. Herein, we use a rat in-vivo strength exercise model with an electrically-induced standardized excitation pattern, previously shown to induce a load-dependent increase in myonuclear number and hypertrophy, to study acute effects of load on molecular signalling. We assessed protein abundance and specific phosphorylation of the four protein kinases FAK, mTOR, p70S6K and JNK after 2, 10 and 28 min of a low- or high-load contraction, in order to assess the effects of load, exercise duration and muscle-type on their response to exercise. Specific phosphorylation of mTOR, p70S6K and JNK was increased after 28 min of exercise under the low- and high-load protocol. Elevated phosphorylation of mTOR and JNK was detectable already after 2 and 10 min of exercise, respectively, but greatest after 28 min of exercise, and JNK phosphorylation was highly load-dependent. The abundance of all four kinases was higher in TA compared to EDL muscle, p70S6K abundance was increased after exercise in a load-independent manner, and FAK and JNK abundance was reduced after 28 min of exercise in both the exercised and control muscles. In conclusion, the current study shows that JNK activation after a single resistance exercise is load-specific, resembling the previously reported degree of myonuclear accrual and muscle hypertrophy with repetition of the exercise stimulus.Einar EftestølMartino V. FranchiStephanie KasperMartin FlückNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Einar Eftestøl Martino V. Franchi Stephanie Kasper Martin Flück JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns
description	Abstract As the excitation–contraction coupling is inseparable during voluntary exercise, the relative contribution of the mechanical and neural input on hypertrophy-related molecular signalling is still poorly understood. Herein, we use a rat in-vivo strength exercise model with an electrically-induced standardized excitation pattern, previously shown to induce a load-dependent increase in myonuclear number and hypertrophy, to study acute effects of load on molecular signalling. We assessed protein abundance and specific phosphorylation of the four protein kinases FAK, mTOR, p70S6K and JNK after 2, 10 and 28 min of a low- or high-load contraction, in order to assess the effects of load, exercise duration and muscle-type on their response to exercise. Specific phosphorylation of mTOR, p70S6K and JNK was increased after 28 min of exercise under the low- and high-load protocol. Elevated phosphorylation of mTOR and JNK was detectable already after 2 and 10 min of exercise, respectively, but greatest after 28 min of exercise, and JNK phosphorylation was highly load-dependent. The abundance of all four kinases was higher in TA compared to EDL muscle, p70S6K abundance was increased after exercise in a load-independent manner, and FAK and JNK abundance was reduced after 28 min of exercise in both the exercised and control muscles. In conclusion, the current study shows that JNK activation after a single resistance exercise is load-specific, resembling the previously reported degree of myonuclear accrual and muscle hypertrophy with repetition of the exercise stimulus.
format	article
author	Einar Eftestøl Martino V. Franchi Stephanie Kasper Martin Flück
author_facet	Einar Eftestøl Martino V. Franchi Stephanie Kasper Martin Flück
author_sort	Einar Eftestøl
title	JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns
title_short	JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns
title_full	JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns
title_fullStr	JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns
title_full_unstemmed	JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns
title_sort	jnk activation in ta and edl muscle is load-dependent in rats receiving identical excitation patterns
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c682d1a9e6ed475f8842813954f2a609
work_keys_str_mv	AT einareftestøl jnkactivationintaandedlmuscleisloaddependentinratsreceivingidenticalexcitationpatterns AT martinovfranchi jnkactivationintaandedlmuscleisloaddependentinratsreceivingidenticalexcitationpatterns AT stephaniekasper jnkactivationintaandedlmuscleisloaddependentinratsreceivingidenticalexcitationpatterns AT martinfluck jnkactivationintaandedlmuscleisloaddependentinratsreceivingidenticalexcitationpatterns
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JNK activation in TA and EDL muscle is load-dependent in rats receiving identical excitation patterns

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