Effects of nanoporous anodic titanium oxide on human adipose derived stem cells

Katarzyna Malec,1 Joanna Góralska,1 Magdalena Hubalewska-Mazgaj,2 Paulina Glowacz,1 Magdalena Jarosz,3 Pawel Brzewski,4 Grzegorz D Sulka,3 Marian Jaskula,3 Iwona Wybranska1 1Department of Clinical Biochemistry, Jagiellonian University Medical College, 2Department of Genetic Research and...

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Autores principales:	Malec K, Góralska J, Hubalewska-Mazgaj M, Głowacz P, Jarosz M, Brzewski P, Sulka GD, Jaskuła M, Wybrańska I
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Adipose derived stem cells anodic titanium oxide nanotopography osteogenic differentiation biomaterials Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c687c9b6798142f08961353cebd33841
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spelling	oai:doaj.org-article:c687c9b6798142f08961353cebd338412021-12-02T00:35:29ZEffects of nanoporous anodic titanium oxide on human adipose derived stem cells1178-2013https://doaj.org/article/c687c9b6798142f08961353cebd338412016-10-01T00:00:00Zhttps://www.dovepress.com/effects-of-nanoporous-anodic-titanium-oxide-on-human-adipose-derived-s-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Katarzyna Malec,1 Joanna Góralska,1 Magdalena Hubalewska-Mazgaj,2 Paulina Glowacz,1 Magdalena Jarosz,3 Pawel Brzewski,4 Grzegorz D Sulka,3 Marian Jaskula,3 Iwona Wybranska1 1Department of Clinical Biochemistry, Jagiellonian University Medical College, 2Department of Genetic Research and Nutrigenomics, Malopolska Centre of Biotechnology, Jagiellonian University, 3Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, 4Department of Dermatology, Jagiellonian University Medical College, Kraków, Poland Abstract: The aim of current bone biomaterials research is to design implants that induce controlled, guided, successful, and rapid healing. Titanium implants are widely used in dental, orthopedic, and reconstructive surgery. A series of studies has indicated that cells can respond not only to the chemical properties of the biomaterial, but also, in particular, to the changes in surface topography. Nanoporous materials remain in focus of scientific queries due to their exclusive properties and broad applications. One such material is nanostructured titanium oxide with highly ordered, mutually perpendicular nanopores. Nanoporous anodic titanium dioxide (TiO2) films were fabricated by a three-step anodization process in propan-1,2,3-triol-based electrolyte containing fluoride ions. Adipose-derived stem cells offer many interesting opportunities for regenerative medicine. The important goal of tissue engineering is to direct stem cell differentiation into a desired cell lineage. The influence of nanoporous TiO2 with pore diameters of 80 and 108 nm on cell response, growth, viability, and ability to differentiate into osteoblastic lineage of human adipose-derived progenitors was explored. Cells were harvested from the subcutaneous abdominal fat tissue by a simple, minimally invasive, and inexpensive method. Our results indicate that anodic nanostructured TiO2 is a safe and nontoxic biomaterial. In vitro studies demonstrated that the nanotopography induced and enhanced osteodifferentiation of human adipose-derived stem cells from the abdominal subcutaneous fat tissue. Keywords: adipose-derived stem cells, anodic titanium oxide, nanotopography, osteogenic differentiation, biomaterialsMalec KGóralska JHubalewska-Mazgaj MGłowacz PJarosz MBrzewski PSulka GDJaskuła MWybrańska IDove Medical PressarticleAdipose derived stem cellsanodic titanium oxidenanotopographyosteogenic differentiationbiomaterialsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 5349-5360 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Adipose derived stem cells anodic titanium oxide nanotopography osteogenic differentiation biomaterials Medicine (General) R5-920
spellingShingle	Adipose derived stem cells anodic titanium oxide nanotopography osteogenic differentiation biomaterials Medicine (General) R5-920 Malec K Góralska J Hubalewska-Mazgaj M Głowacz P Jarosz M Brzewski P Sulka GD Jaskuła M Wybrańska I Effects of nanoporous anodic titanium oxide on human adipose derived stem cells
description	Katarzyna Malec,1 Joanna Góralska,1 Magdalena Hubalewska-Mazgaj,2 Paulina Glowacz,1 Magdalena Jarosz,3 Pawel Brzewski,4 Grzegorz D Sulka,3 Marian Jaskula,3 Iwona Wybranska1 1Department of Clinical Biochemistry, Jagiellonian University Medical College, 2Department of Genetic Research and Nutrigenomics, Malopolska Centre of Biotechnology, Jagiellonian University, 3Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, 4Department of Dermatology, Jagiellonian University Medical College, Kraków, Poland Abstract: The aim of current bone biomaterials research is to design implants that induce controlled, guided, successful, and rapid healing. Titanium implants are widely used in dental, orthopedic, and reconstructive surgery. A series of studies has indicated that cells can respond not only to the chemical properties of the biomaterial, but also, in particular, to the changes in surface topography. Nanoporous materials remain in focus of scientific queries due to their exclusive properties and broad applications. One such material is nanostructured titanium oxide with highly ordered, mutually perpendicular nanopores. Nanoporous anodic titanium dioxide (TiO2) films were fabricated by a three-step anodization process in propan-1,2,3-triol-based electrolyte containing fluoride ions. Adipose-derived stem cells offer many interesting opportunities for regenerative medicine. The important goal of tissue engineering is to direct stem cell differentiation into a desired cell lineage. The influence of nanoporous TiO2 with pore diameters of 80 and 108 nm on cell response, growth, viability, and ability to differentiate into osteoblastic lineage of human adipose-derived progenitors was explored. Cells were harvested from the subcutaneous abdominal fat tissue by a simple, minimally invasive, and inexpensive method. Our results indicate that anodic nanostructured TiO2 is a safe and nontoxic biomaterial. In vitro studies demonstrated that the nanotopography induced and enhanced osteodifferentiation of human adipose-derived stem cells from the abdominal subcutaneous fat tissue. Keywords: adipose-derived stem cells, anodic titanium oxide, nanotopography, osteogenic differentiation, biomaterials
format	article
author	Malec K Góralska J Hubalewska-Mazgaj M Głowacz P Jarosz M Brzewski P Sulka GD Jaskuła M Wybrańska I
author_facet	Malec K Góralska J Hubalewska-Mazgaj M Głowacz P Jarosz M Brzewski P Sulka GD Jaskuła M Wybrańska I
author_sort	Malec K
title	Effects of nanoporous anodic titanium oxide on human adipose derived stem cells
title_short	Effects of nanoporous anodic titanium oxide on human adipose derived stem cells
title_full	Effects of nanoporous anodic titanium oxide on human adipose derived stem cells
title_fullStr	Effects of nanoporous anodic titanium oxide on human adipose derived stem cells
title_full_unstemmed	Effects of nanoporous anodic titanium oxide on human adipose derived stem cells
title_sort	effects of nanoporous anodic titanium oxide on human adipose derived stem cells
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/c687c9b6798142f08961353cebd33841
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Effects of nanoporous anodic titanium oxide on human adipose derived stem cells

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