Role of microRNA-26b in glioma development and its mediated regulation on EphA2.

<h4>Background</h4>MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has...

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Autores principales:	Ning Wu, Xiangzhong Zhao, Ming Liu, Haizhou Liu, Weicheng Yao, Yuyan Zhang, Shousong Cao, Xiukun Lin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c68cb697e0da45b19091fdc258a65bb7
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spelling	oai:doaj.org-article:c68cb697e0da45b19091fdc258a65bb72021-11-18T07:00:27ZRole of microRNA-26b in glioma development and its mediated regulation on EphA2.1932-620310.1371/journal.pone.0016264https://doaj.org/article/c68cb697e0da45b19091fdc258a65bb72011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21264258/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined.<h4>Methodology/principal findings</h4>Real-time PCR was employed to measure the expression level of miR-26b in glioma patients and cells. The level of miR-26b was inversely correlated with the grade of glioma. Ectopic expression of miR-26b inhibited the proliferation, migration and invasion of human glioma cells. A binding site for miR-26b was identified in the 3'UTR of EphA2. Over-expression of miR-26b in glioma cells repressed the endogenous level of EphA2 protein. Vasculogenic mimicry (VM) experiments were performed to further confirm the effects of miR-26b on the regulation of EphA2, and the results showed that miR-26b inhibited the VM processes which regulated by EphA2.<h4>Significance</h4>This study demonstrated that miR-26b may act as a tumor suppressor in glioma and it directly regulates EphA2 expression. EphA2 is a direct target of miR-26b, and the down-regulation of EphA2 mediated by miR-26b is dependent on the binding of miR-26b to a specific response element of microRNA in the 3'UTR region of EphA2 mRNA.Ning WuXiangzhong ZhaoMing LiuHaizhou LiuWeicheng YaoYuyan ZhangShousong CaoXiukun LinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e16264 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ning Wu Xiangzhong Zhao Ming Liu Haizhou Liu Weicheng Yao Yuyan Zhang Shousong Cao Xiukun Lin Role of microRNA-26b in glioma development and its mediated regulation on EphA2.
description	<h4>Background</h4>MicroRNAs (miRNAs) are short, non-coding RNAs that regulate the expression of multiple target genes. Deregulation of miRNAs is common in human tumorigenesis. Low level expression of miR-26b has been found in glioma cells. However, its underlying mechanism of action has not been determined.<h4>Methodology/principal findings</h4>Real-time PCR was employed to measure the expression level of miR-26b in glioma patients and cells. The level of miR-26b was inversely correlated with the grade of glioma. Ectopic expression of miR-26b inhibited the proliferation, migration and invasion of human glioma cells. A binding site for miR-26b was identified in the 3'UTR of EphA2. Over-expression of miR-26b in glioma cells repressed the endogenous level of EphA2 protein. Vasculogenic mimicry (VM) experiments were performed to further confirm the effects of miR-26b on the regulation of EphA2, and the results showed that miR-26b inhibited the VM processes which regulated by EphA2.<h4>Significance</h4>This study demonstrated that miR-26b may act as a tumor suppressor in glioma and it directly regulates EphA2 expression. EphA2 is a direct target of miR-26b, and the down-regulation of EphA2 mediated by miR-26b is dependent on the binding of miR-26b to a specific response element of microRNA in the 3'UTR region of EphA2 mRNA.
format	article
author	Ning Wu Xiangzhong Zhao Ming Liu Haizhou Liu Weicheng Yao Yuyan Zhang Shousong Cao Xiukun Lin
author_facet	Ning Wu Xiangzhong Zhao Ming Liu Haizhou Liu Weicheng Yao Yuyan Zhang Shousong Cao Xiukun Lin
author_sort	Ning Wu
title	Role of microRNA-26b in glioma development and its mediated regulation on EphA2.
title_short	Role of microRNA-26b in glioma development and its mediated regulation on EphA2.
title_full	Role of microRNA-26b in glioma development and its mediated regulation on EphA2.
title_fullStr	Role of microRNA-26b in glioma development and its mediated regulation on EphA2.
title_full_unstemmed	Role of microRNA-26b in glioma development and its mediated regulation on EphA2.
title_sort	role of microrna-26b in glioma development and its mediated regulation on epha2.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c68cb697e0da45b19091fdc258a65bb7
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Role of microRNA-26b in glioma development and its mediated regulation on EphA2.

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