Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.

The transthyretin-like protein (TLP) from Salmonella enterica subspecies I is a periplasmic protein with high level structural similarity to a protein found in mammals and fish. In humans, the protein homologue, transthyretin, binds and carries retinol and thyroxine, and a series of other, unrelated...

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Autores principales:	Sarah C Hennebry, Leanne C Sait, Raju Mantena, Thomas J Humphrey, Ji Yang, Timothy Scott, Andreas Kupz, Samantha J Richardson, Richard A Strugnell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c6917a5047c74293b89cc78bc255b155
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spelling	oai:doaj.org-article:c6917a5047c74293b89cc78bc255b1552021-11-18T08:04:00ZSalmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.1932-620310.1371/journal.pone.0046675https://doaj.org/article/c6917a5047c74293b89cc78bc255b1552012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23284609/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The transthyretin-like protein (TLP) from Salmonella enterica subspecies I is a periplasmic protein with high level structural similarity to a protein found in mammals and fish. In humans, the protein homologue, transthyretin, binds and carries retinol and thyroxine, and a series of other, unrelated aromatic compounds. Here we show that the amino acid sequence of the TLP from different species, subspecies and serovars of the Salmonella genus is highly conserved and demonstrate that the TLP gene is constitutively expressed in S. Typhimurium and that copper and other divalent metal ions severely inhibit enzyme activity of the TLP, a cyclic amidohydrolase that hydrolyses 5-hydroxyisourate (5-HIU). In order to determine the in vivo role of the S. Typhimurium TLP, we constructed a strain of mouse-virulent S. Typhimurium SL1344 bearing a mutation in the TLP gene (SL1344 ΔyedX). We assessed the virulence of this strain via oral inoculation of mice and chickens. Whilst SL1344 ΔyedX induced a systemic infection in both organisms, the bacterial load detected in the faeces of infected chickens was significantly reduced when compared to the load of S. Typhimurium SL1344. These data demonstrate that the TLP gene is required for survival of S. Typhimurium in a high uric acid environment such as chicken faeces, and that metabolic traits of Salmonellae in natural and contrived hosts may be fundamentally different. Our data also highlight the importance of using appropriate animal models for the study of bacterial pathogenesis especially where host-specific virulence factors or traits are the subject of the study.Sarah C HennebryLeanne C SaitRaju MantenaThomas J HumphreyJi YangTimothy ScottAndreas KupzSamantha J RichardsonRichard A StrugnellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e46675 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sarah C Hennebry Leanne C Sait Raju Mantena Thomas J Humphrey Ji Yang Timothy Scott Andreas Kupz Samantha J Richardson Richard A Strugnell Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
description	The transthyretin-like protein (TLP) from Salmonella enterica subspecies I is a periplasmic protein with high level structural similarity to a protein found in mammals and fish. In humans, the protein homologue, transthyretin, binds and carries retinol and thyroxine, and a series of other, unrelated aromatic compounds. Here we show that the amino acid sequence of the TLP from different species, subspecies and serovars of the Salmonella genus is highly conserved and demonstrate that the TLP gene is constitutively expressed in S. Typhimurium and that copper and other divalent metal ions severely inhibit enzyme activity of the TLP, a cyclic amidohydrolase that hydrolyses 5-hydroxyisourate (5-HIU). In order to determine the in vivo role of the S. Typhimurium TLP, we constructed a strain of mouse-virulent S. Typhimurium SL1344 bearing a mutation in the TLP gene (SL1344 ΔyedX). We assessed the virulence of this strain via oral inoculation of mice and chickens. Whilst SL1344 ΔyedX induced a systemic infection in both organisms, the bacterial load detected in the faeces of infected chickens was significantly reduced when compared to the load of S. Typhimurium SL1344. These data demonstrate that the TLP gene is required for survival of S. Typhimurium in a high uric acid environment such as chicken faeces, and that metabolic traits of Salmonellae in natural and contrived hosts may be fundamentally different. Our data also highlight the importance of using appropriate animal models for the study of bacterial pathogenesis especially where host-specific virulence factors or traits are the subject of the study.
format	article
author	Sarah C Hennebry Leanne C Sait Raju Mantena Thomas J Humphrey Ji Yang Timothy Scott Andreas Kupz Samantha J Richardson Richard A Strugnell
author_facet	Sarah C Hennebry Leanne C Sait Raju Mantena Thomas J Humphrey Ji Yang Timothy Scott Andreas Kupz Samantha J Richardson Richard A Strugnell
author_sort	Sarah C Hennebry
title	Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
title_short	Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
title_full	Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
title_fullStr	Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
title_full_unstemmed	Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
title_sort	salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c6917a5047c74293b89cc78bc255b155
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Salmonella typhimurium's transthyretin-like protein is a host-specific factor important in fecal survival in chickens.

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