Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner

Macrophage is essential for host anti-bacterial defense by directly eliminating invading microbes and inducing a series of immune reactions. Here we identified a Streptococcus pneumoniae protein, PepO, as a TLR2/TLR4 bi-ligand. We found that PepO enhances macrophage unspecific phagocytosis and bacte...

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Autores principales:	Zhaoche Shu, Jun Yuan, Hong Wang, Jinghui Zhang, Sijie Li, Hong Zhang, Yusi Liu, Yibing Yin, Xuemei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	bacterial infection toll-like receptor macrophage autophagy immunotherapy Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/c692bbc601774142a5344d482249723a
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spelling	oai:doaj.org-article:c692bbc601774142a5344d482249723a2021-11-17T14:21:58ZStreptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner2150-55942150-560810.1080/21505594.2020.1739411https://doaj.org/article/c692bbc601774142a5344d482249723a2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1739411https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Macrophage is essential for host anti-bacterial defense by directly eliminating invading microbes and inducing a series of immune reactions. Here we identified a Streptococcus pneumoniae protein, PepO, as a TLR2/TLR4 bi-ligand. We found that PepO enhances macrophage unspecific phagocytosis and bactericidal activity, which is related to the induction of autophagy in macrophage, for the inhibition of autophagy significantly decreased the phagocytosis and bactericidal activity of PepO-treated macrophage. We confirmed that these effects of PepO are dependent on interacting with both TLR2 and TLR4. The tlr2 or tlr4 deficiency partially abolished the effect of PepO while tlr2/tlr4 deficiency abolished it completely. In vivo study demonstrated that PepO reduced the bacteria load in WT mice significantly, while the depletion of macrophage or tlr2/tlr4 deficiency abrogated the effect of PepO. Our findings suggested the therapeutic potential of PepO and provided experimental evidence for immunotherapy against infectious disease.Zhaoche ShuJun YuanHong WangJinghui ZhangSijie LiHong ZhangYusi LiuYibing YinXuemei ZhangTaylor & Francis Grouparticlebacterial infectiontoll-like receptormacrophageautophagyimmunotherapyInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 270-282 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial infection toll-like receptor macrophage autophagy immunotherapy Infectious and parasitic diseases RC109-216
spellingShingle	bacterial infection toll-like receptor macrophage autophagy immunotherapy Infectious and parasitic diseases RC109-216 Zhaoche Shu Jun Yuan Hong Wang Jinghui Zhang Sijie Li Hong Zhang Yusi Liu Yibing Yin Xuemei Zhang Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner
description	Macrophage is essential for host anti-bacterial defense by directly eliminating invading microbes and inducing a series of immune reactions. Here we identified a Streptococcus pneumoniae protein, PepO, as a TLR2/TLR4 bi-ligand. We found that PepO enhances macrophage unspecific phagocytosis and bactericidal activity, which is related to the induction of autophagy in macrophage, for the inhibition of autophagy significantly decreased the phagocytosis and bactericidal activity of PepO-treated macrophage. We confirmed that these effects of PepO are dependent on interacting with both TLR2 and TLR4. The tlr2 or tlr4 deficiency partially abolished the effect of PepO while tlr2/tlr4 deficiency abolished it completely. In vivo study demonstrated that PepO reduced the bacteria load in WT mice significantly, while the depletion of macrophage or tlr2/tlr4 deficiency abrogated the effect of PepO. Our findings suggested the therapeutic potential of PepO and provided experimental evidence for immunotherapy against infectious disease.
format	article
author	Zhaoche Shu Jun Yuan Hong Wang Jinghui Zhang Sijie Li Hong Zhang Yusi Liu Yibing Yin Xuemei Zhang
author_facet	Zhaoche Shu Jun Yuan Hong Wang Jinghui Zhang Sijie Li Hong Zhang Yusi Liu Yibing Yin Xuemei Zhang
author_sort	Zhaoche Shu
title	Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner
title_short	Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner
title_full	Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner
title_fullStr	Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner
title_full_unstemmed	Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner
title_sort	streptococcus pneumoniae pepo promotes host anti-infection defense via autophagy in a toll-like receptor 2/4 dependent manner
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/c692bbc601774142a5344d482249723a
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Streptococcus pneumoniae PepO promotes host anti-infection defense via autophagy in a Toll-like receptor 2/4 dependent manner

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