Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis
ABSTRACT Bacillus endophthalmitis is a devastating eye infection that causes rapid blindness through extracellular tissue-destructive exotoxins. Despite its importance, knowledge of the phylogenetic relationships and population structure of intraocular Bacillus spp. is lacking. In this study, we seq...
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American Society for Microbiology
2019
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oai:doaj.org-article:c6955df8821147e5825c69924ed469902021-12-02T18:39:15ZMolecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis10.1128/mSystems.00745-192379-5077https://doaj.org/article/c6955df8821147e5825c69924ed469902019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00745-19https://doaj.org/toc/2379-5077ABSTRACT Bacillus endophthalmitis is a devastating eye infection that causes rapid blindness through extracellular tissue-destructive exotoxins. Despite its importance, knowledge of the phylogenetic relationships and population structure of intraocular Bacillus spp. is lacking. In this study, we sequenced the whole genomes of eight Bacillus intraocular pathogens independently isolated from 8/52 patients with posttraumatic Bacillus endophthalmitis infections in the Eye Hospital of Wenzhou Medical University between January 2010 and December 2018. Phylogenetic analysis revealed that the pathogenic intraocular isolates belonged to Bacillus cereus, Bacillus thuringiensis and Bacillus toyonensis. To determine the virulence of the ocular isolates, three representative strains were injected into mouse models, and severe endophthalmitis leading to blindness was observed. Through incorporating publicly available genomes for Bacillus spp., we found that the intraocular pathogens could be isolated independently but displayed a similar genetic context. In addition, our data provide genome-wide support for intraocular and gastrointestinal sources of Bacillus spp. belonging to different lineages. Importantly, we identified five molecular signatures of virulence and motility genes associated with intraocular infection, namely, plcA-2, InhA-3, InhA-4, hblA-5, and fliD using pangenome-wide association studies. The characterization of overrepresented genes in the intraocular isolates holds value to predict bacterial evolution and for the design of future intervention strategies in patients with endophthalmitis. IMPORTANCE In this study, we provided a detailed and comprehensive clinicopathological and pathogenic report of Bacillus endophthalmitis over the 8 years of the study period. We first reported the whole-genome sequence of Bacillus spp. causing devastating endophthalmitis and found that Bacillus toyonensis is able to cause endophthalmitis. Finally, we revealed significant endophthalmitis-associated virulence genes involved in hemolysis, immunity inhibition, and pathogenesis. Overall, as more sequencing data sets become available, these data will facilitate comparative research and will reveal the emergence of pathogenic “ocular bacteria.”Jian YuanYu-Yu LiYi XuBian-Jin SunJiao ShaoDie ZhangKai LiDan-Dan FanZheng-Bo XueWei-Hua ChenClara PakYong-Liang LouJian-Zhong SuMei-Qin ZhengAmerican Society for MicrobiologyarticleendophthalmitisBacilluspangenomevirulenceevolutionMicrobiologyQR1-502ENmSystems, Vol 4, Iss 6 (2019) |
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endophthalmitis Bacillus pangenome virulence evolution Microbiology QR1-502 |
| spellingShingle |
endophthalmitis Bacillus pangenome virulence evolution Microbiology QR1-502 Jian Yuan Yu-Yu Li Yi Xu Bian-Jin Sun Jiao Shao Die Zhang Kai Li Dan-Dan Fan Zheng-Bo Xue Wei-Hua Chen Clara Pak Yong-Liang Lou Jian-Zhong Su Mei-Qin Zheng Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis |
| description |
ABSTRACT Bacillus endophthalmitis is a devastating eye infection that causes rapid blindness through extracellular tissue-destructive exotoxins. Despite its importance, knowledge of the phylogenetic relationships and population structure of intraocular Bacillus spp. is lacking. In this study, we sequenced the whole genomes of eight Bacillus intraocular pathogens independently isolated from 8/52 patients with posttraumatic Bacillus endophthalmitis infections in the Eye Hospital of Wenzhou Medical University between January 2010 and December 2018. Phylogenetic analysis revealed that the pathogenic intraocular isolates belonged to Bacillus cereus, Bacillus thuringiensis and Bacillus toyonensis. To determine the virulence of the ocular isolates, three representative strains were injected into mouse models, and severe endophthalmitis leading to blindness was observed. Through incorporating publicly available genomes for Bacillus spp., we found that the intraocular pathogens could be isolated independently but displayed a similar genetic context. In addition, our data provide genome-wide support for intraocular and gastrointestinal sources of Bacillus spp. belonging to different lineages. Importantly, we identified five molecular signatures of virulence and motility genes associated with intraocular infection, namely, plcA-2, InhA-3, InhA-4, hblA-5, and fliD using pangenome-wide association studies. The characterization of overrepresented genes in the intraocular isolates holds value to predict bacterial evolution and for the design of future intervention strategies in patients with endophthalmitis. IMPORTANCE In this study, we provided a detailed and comprehensive clinicopathological and pathogenic report of Bacillus endophthalmitis over the 8 years of the study period. We first reported the whole-genome sequence of Bacillus spp. causing devastating endophthalmitis and found that Bacillus toyonensis is able to cause endophthalmitis. Finally, we revealed significant endophthalmitis-associated virulence genes involved in hemolysis, immunity inhibition, and pathogenesis. Overall, as more sequencing data sets become available, these data will facilitate comparative research and will reveal the emergence of pathogenic “ocular bacteria.” |
| format |
article |
| author |
Jian Yuan Yu-Yu Li Yi Xu Bian-Jin Sun Jiao Shao Die Zhang Kai Li Dan-Dan Fan Zheng-Bo Xue Wei-Hua Chen Clara Pak Yong-Liang Lou Jian-Zhong Su Mei-Qin Zheng |
| author_facet |
Jian Yuan Yu-Yu Li Yi Xu Bian-Jin Sun Jiao Shao Die Zhang Kai Li Dan-Dan Fan Zheng-Bo Xue Wei-Hua Chen Clara Pak Yong-Liang Lou Jian-Zhong Su Mei-Qin Zheng |
| author_sort |
Jian Yuan |
| title |
Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis |
| title_short |
Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis |
| title_full |
Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis |
| title_fullStr |
Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis |
| title_full_unstemmed |
Molecular Signatures Related to the Virulence of <named-content content-type="genus-species">Bacillus cereus Sensu Lato</named-content>, a Leading Cause of Devastating Endophthalmitis |
| title_sort |
molecular signatures related to the virulence of <named-content content-type="genus-species">bacillus cereus sensu lato</named-content>, a leading cause of devastating endophthalmitis |
| publisher |
American Society for Microbiology |
| publishDate |
2019 |
| url |
https://doaj.org/article/c6955df8821147e5825c69924ed46990 |
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