Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis

Iman Kotb,1 Caroline Meharg,2 Robert N Barker,1 Anthony Ormerod11Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK; 2Institute for Global Food Security, Queen's University, Belfast, Northern IrelandAbstract: Identifying differential expression of genes in psoriat...

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Autores principales:	Kotb I, Meharg C, Barker RN, Ormerod A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Dermatology RL1-803
Acceso en línea:	https://doaj.org/article/c6977c3512954c679b389d5945dfe059
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spelling	oai:doaj.org-article:c6977c3512954c679b389d5945dfe0592021-12-02T08:58:02ZReanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis2230-326Xhttps://doaj.org/article/c6977c3512954c679b389d5945dfe0592013-12-01T00:00:00Zhttp://www.dovepress.com/reanalysis-of-microarray-data-reveals-insights-into-altered-transcript-a15270https://doaj.org/toc/2230-326X Iman Kotb,1 Caroline Meharg,2 Robert N Barker,1 Anthony Ormerod11Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK; 2Institute for Global Food Security, Queen's University, Belfast, Northern IrelandAbstract: Identifying differential expression of genes in psoriatic and healthy skin by microarray data analysis is a key approach to understand the pathogenesis of psoriasis. Analysis of more than one dataset to identify genes commonly upregulated reduces the likelihood of false positives and narrows down the possible signature genes. Genes controlling the critical balance between T helper 17 and regulatory T cells are of special interest in psoriasis. Our objectives were to identify genes that are consistently upregulated in lesional skin from three published microarray datasets. We carried out a reanalysis of gene expression data extracted from three experiments on samples from psoriatic and nonlesional skin using the same stringency threshold and software and further compared the expression levels of 92 genes related to the T helper 17 and regulatory T cell signaling pathways. We found 73 probe sets representing 57 genes commonly upregulated in lesional skin from all datasets. These included 26 probe sets representing 20 genes that have no previous link to the etiopathogenesis of psoriasis. These genes may represent novel therapeutic targets and surely need more rigorous experimental testing to be validated. Our analysis also identified 12 of 92 genes known to be related to the T helper 17 and regulatory T cell signaling pathways, and these were found to be differentially expressed in the lesional skin samples.Keywords: psoriasis, gene array analysis, gene expression profilingKotb IMeharg CBarker RNOrmerod ADove Medical PressarticleDermatologyRL1-803ENPsoriasis: Targets and Therapy, Vol 2013, Iss default, Pp 31-47 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dermatology RL1-803
spellingShingle	Dermatology RL1-803 Kotb I Meharg C Barker RN Ormerod A Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis
description	Iman Kotb,1 Caroline Meharg,2 Robert N Barker,1 Anthony Ormerod11Institute of Medical Sciences, University of Aberdeen, Aberdeen, Scotland, UK; 2Institute for Global Food Security, Queen's University, Belfast, Northern IrelandAbstract: Identifying differential expression of genes in psoriatic and healthy skin by microarray data analysis is a key approach to understand the pathogenesis of psoriasis. Analysis of more than one dataset to identify genes commonly upregulated reduces the likelihood of false positives and narrows down the possible signature genes. Genes controlling the critical balance between T helper 17 and regulatory T cells are of special interest in psoriasis. Our objectives were to identify genes that are consistently upregulated in lesional skin from three published microarray datasets. We carried out a reanalysis of gene expression data extracted from three experiments on samples from psoriatic and nonlesional skin using the same stringency threshold and software and further compared the expression levels of 92 genes related to the T helper 17 and regulatory T cell signaling pathways. We found 73 probe sets representing 57 genes commonly upregulated in lesional skin from all datasets. These included 26 probe sets representing 20 genes that have no previous link to the etiopathogenesis of psoriasis. These genes may represent novel therapeutic targets and surely need more rigorous experimental testing to be validated. Our analysis also identified 12 of 92 genes known to be related to the T helper 17 and regulatory T cell signaling pathways, and these were found to be differentially expressed in the lesional skin samples.Keywords: psoriasis, gene array analysis, gene expression profiling
format	article
author	Kotb I Meharg C Barker RN Ormerod A
author_facet	Kotb I Meharg C Barker RN Ormerod A
author_sort	Kotb I
title	Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis
title_short	Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis
title_full	Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis
title_fullStr	Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis
title_full_unstemmed	Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis
title_sort	reanalysis of microarray data reveals insights into altered transcriptional activity of t helper 17 and regulatory t cell signaling in psoriasis
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/c6977c3512954c679b389d5945dfe059
work_keys_str_mv	AT kotbi reanalysisofmicroarraydatarevealsinsightsintoalteredtranscriptionalactivityofthelper17andregulatorytcellsignalinginpsoriasis AT mehargc reanalysisofmicroarraydatarevealsinsightsintoalteredtranscriptionalactivityofthelper17andregulatorytcellsignalinginpsoriasis AT barkerrn reanalysisofmicroarraydatarevealsinsightsintoalteredtranscriptionalactivityofthelper17andregulatorytcellsignalinginpsoriasis AT ormeroda reanalysisofmicroarraydatarevealsinsightsintoalteredtranscriptionalactivityofthelper17andregulatorytcellsignalinginpsoriasis
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Reanalysis of microarray data reveals insights into altered transcriptional activity of T helper 17 and regulatory T cell signaling in psoriasis

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