Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia

Aeny TN, Prasetyo J, Suharjo R, Dirmawati SR, Efri, Niswati A. 2018. Isolation and Identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia. Biodiversitas 19: 2052-2058. This study was aimed to collect and identify actinomycetes from the rh...

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Autores principales:	TITIK NUR AENY, JOKO PRASETYO, RADIX SUHARJO, SUSKANDINI R. DIRMAWATI, EFRI EFRI, AININ NISWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	actinomycetes ananas comosus antagonist dickeya zeae streptomyces Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c6a398f88bce4d1c9b78b8bad1e6ee41
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spelling	oai:doaj.org-article:c6a398f88bce4d1c9b78b8bad1e6ee412021-11-16T14:04:41ZShort Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia1412-033X2085-472210.13057/biodiv/d190610https://doaj.org/article/c6a398f88bce4d1c9b78b8bad1e6ee412018-10-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3083https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Aeny TN, Prasetyo J, Suharjo R, Dirmawati SR, Efri, Niswati A. 2018. Isolation and Identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia. Biodiversitas 19: 2052-2058. This study was aimed to collect and identify actinomycetes from the rhizosphere of pineapple with the potency as a biocontrol agent of Dickeya zeae, the pathogen of pineapple soft rot. Soil samples were collected from four different pineapple plantations in Lampung Province, in December 2016. Samples were air-dried, serially diluted, and plated on modified ISP2 medium and several single characterized colonies were sub cultured and purified. The growing colonies were grouped into different colour series based on their aerial-mycelia and substrate-mycelia colors. Antagonistic activity of the actinomycetes isolates were evaluated against Dickeya zeae by agar diffusion method using the same medium agar lawn with the bacterial cultures. Selected antagonistic actinomycete isolates with the biggest clear zone formation were further characterized and identified molecularly based on 16S rDNA sequences. The results showed that as many as 34 actinomycete isolates collected from pineapple rhizosphere produced clear zones. Two isolates, namely GGF2-i5 and GGF4-i18, presented the biggest clear zone were proposed as the potential antagonist strains against D. zeae and identified as Streptomyces parvulus and Streptomyces hygroscopicus subsp. hygroscopicus, respectively. Both of the 16S rRNA gene sequences from those strains were deposited in the GenBank with the accession number MH170279 and MH170280, respectively.TITIK NUR AENYJOKO PRASETYORADIX SUHARJOSUSKANDINI R. DIRMAWATIEFRI EFRIAININ NISWATIMBI & UNS Soloarticleactinomycetesananas comosusantagonistdickeya zeaestreptomycesBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 6, Pp 2052-2058 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	actinomycetes ananas comosus antagonist dickeya zeae streptomyces Biology (General) QH301-705.5
spellingShingle	actinomycetes ananas comosus antagonist dickeya zeae streptomyces Biology (General) QH301-705.5 TITIK NUR AENY JOKO PRASETYO RADIX SUHARJO SUSKANDINI R. DIRMAWATI EFRI EFRI AININ NISWATI Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia
description	Aeny TN, Prasetyo J, Suharjo R, Dirmawati SR, Efri, Niswati A. 2018. Isolation and Identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia. Biodiversitas 19: 2052-2058. This study was aimed to collect and identify actinomycetes from the rhizosphere of pineapple with the potency as a biocontrol agent of Dickeya zeae, the pathogen of pineapple soft rot. Soil samples were collected from four different pineapple plantations in Lampung Province, in December 2016. Samples were air-dried, serially diluted, and plated on modified ISP2 medium and several single characterized colonies were sub cultured and purified. The growing colonies were grouped into different colour series based on their aerial-mycelia and substrate-mycelia colors. Antagonistic activity of the actinomycetes isolates were evaluated against Dickeya zeae by agar diffusion method using the same medium agar lawn with the bacterial cultures. Selected antagonistic actinomycete isolates with the biggest clear zone formation were further characterized and identified molecularly based on 16S rDNA sequences. The results showed that as many as 34 actinomycete isolates collected from pineapple rhizosphere produced clear zones. Two isolates, namely GGF2-i5 and GGF4-i18, presented the biggest clear zone were proposed as the potential antagonist strains against D. zeae and identified as Streptomyces parvulus and Streptomyces hygroscopicus subsp. hygroscopicus, respectively. Both of the 16S rRNA gene sequences from those strains were deposited in the GenBank with the accession number MH170279 and MH170280, respectively.
format	article
author	TITIK NUR AENY JOKO PRASETYO RADIX SUHARJO SUSKANDINI R. DIRMAWATI EFRI EFRI AININ NISWATI
author_facet	TITIK NUR AENY JOKO PRASETYO RADIX SUHARJO SUSKANDINI R. DIRMAWATI EFRI EFRI AININ NISWATI
author_sort	TITIK NUR AENY
title	Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia
title_short	Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia
title_full	Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia
title_fullStr	Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia
title_full_unstemmed	Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia
title_sort	short communication: isolation and identification of actinomycetes potential as the antagonist of dickeya zeae pineapple soft rot in lampung, indonesia
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/c6a398f88bce4d1c9b78b8bad1e6ee41
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Short Communication: Isolation and identification of actinomycetes potential as the antagonist of Dickeya zeae pineapple soft rot in Lampung, Indonesia

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