One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles

Jin Hyun Kim,1,* Hyun Hoon Chung,2,* Min Sook Jeong,1 Mi Ryoung Song,1 Keon Wook Kang,3,4 Jun Sung Kim1 1R&D Center, Biterials Co, Ltd, Seoul, Republic of Korea; 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea; 3Department of...

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Autores principales:	Kim JH, Chung HH, Jeong MS, Song MR, Kang KW, Kim JS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c6a900697d9344c8a45679e6475a35c5
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spelling	oai:doaj.org-article:c6a900697d9344c8a45679e6475a35c52021-12-02T05:11:30ZOne-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles1176-91141178-2013https://doaj.org/article/c6a900697d9344c8a45679e6475a35c52013-06-01T00:00:00Zhttp://www.dovepress.com/one-step-detection-of-circulating-tumor-cells-in-ovarian-cancer-using--a13426https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jin Hyun Kim,1,* Hyun Hoon Chung,2,* Min Sook Jeong,1 Mi Ryoung Song,1 Keon Wook Kang,3,4 Jun Sung Kim1 1R&D Center, Biterials Co, Ltd, Seoul, Republic of Korea; 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea; 3Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; 4Cancer Research Institute, Seoul National University, Seoul, Republic of Korea *These authors contributed equally to this work Abstract: Ovarian cancer is the fifth-leading cause of cancer-related deaths among women as a result of late diagnosis. For survival rates to improve, more sensitive and specific methods for earlier detection of ovarian cancer are needed. This study presents the development of rapid and specific one-step circulating tumor cell (CTC) detection using flow cytometry in a whole-blood sample with fluorescent silica nanoparticles. We prepared magnetic nanoparticle (MNP)-SiO2(rhodamine B isothiocyanate [RITC]) (MNP-SiO2[RITC] incorporating organic dyes [RITC, λmax(ex/em) = 543/580 nm]) in the silica shell. We then controlled the amount of organic dye in the silica shell of MNP-SiO2(RITC) for increased fluorescence intensity to overcome the autofluorescence of whole blood and increase the sensitivity of CTC detection in whole blood. Next, we modified the surface function group of MNP-SiO2(RITC) from –OH to polyethylene glycol (PEG)/COOH and conjugated a mucin 1 cell surface-associated (MUC1) antibody on the surface of MNP-SiO2(RITC) for CTC detection. To study the specific targeting efficiency of MUC1-MNP-SiO2(RITC), we used immunocytochemistry with a MUC1-positive human ovarian cancer cell line and a negative human embryonic kidney cell line. This technology was capable of detecting 100 ovarian cancer cells in 50 µL of whole blood. In conclusion, we developed a one-step CTC detection technology in ovarian cancer based on multifunctional silica nanoparticles and the use of flow cytometry. Keywords: circulating tumor cells, CTCs, fluorescent nanoprobe, MUC1, ovarian cancerKim JHChung HHJeong MSSong MRKang KWKim JSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2247-2257 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Kim JH Chung HH Jeong MS Song MR Kang KW Kim JS One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
description	Jin Hyun Kim,1,* Hyun Hoon Chung,2,* Min Sook Jeong,1 Mi Ryoung Song,1 Keon Wook Kang,3,4 Jun Sung Kim1 1R&D Center, Biterials Co, Ltd, Seoul, Republic of Korea; 2Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea; 3Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; 4Cancer Research Institute, Seoul National University, Seoul, Republic of Korea *These authors contributed equally to this work Abstract: Ovarian cancer is the fifth-leading cause of cancer-related deaths among women as a result of late diagnosis. For survival rates to improve, more sensitive and specific methods for earlier detection of ovarian cancer are needed. This study presents the development of rapid and specific one-step circulating tumor cell (CTC) detection using flow cytometry in a whole-blood sample with fluorescent silica nanoparticles. We prepared magnetic nanoparticle (MNP)-SiO2(rhodamine B isothiocyanate [RITC]) (MNP-SiO2[RITC] incorporating organic dyes [RITC, λmax(ex/em) = 543/580 nm]) in the silica shell. We then controlled the amount of organic dye in the silica shell of MNP-SiO2(RITC) for increased fluorescence intensity to overcome the autofluorescence of whole blood and increase the sensitivity of CTC detection in whole blood. Next, we modified the surface function group of MNP-SiO2(RITC) from –OH to polyethylene glycol (PEG)/COOH and conjugated a mucin 1 cell surface-associated (MUC1) antibody on the surface of MNP-SiO2(RITC) for CTC detection. To study the specific targeting efficiency of MUC1-MNP-SiO2(RITC), we used immunocytochemistry with a MUC1-positive human ovarian cancer cell line and a negative human embryonic kidney cell line. This technology was capable of detecting 100 ovarian cancer cells in 50 µL of whole blood. In conclusion, we developed a one-step CTC detection technology in ovarian cancer based on multifunctional silica nanoparticles and the use of flow cytometry. Keywords: circulating tumor cells, CTCs, fluorescent nanoprobe, MUC1, ovarian cancer
format	article
author	Kim JH Chung HH Jeong MS Song MR Kang KW Kim JS
author_facet	Kim JH Chung HH Jeong MS Song MR Kang KW Kim JS
author_sort	Kim JH
title	One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
title_short	One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
title_full	One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
title_fullStr	One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
title_full_unstemmed	One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
title_sort	one-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/c6a900697d9344c8a45679e6475a35c5
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One-step detection of circulating tumor cells in ovarian cancer using enhanced fluorescent silica nanoparticles

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