Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells

Abstract Rab46 is a novel Ca2+-sensing Rab GTPase shown to have important functions in endothelial and immune cells. The presence of functional Ca2+-binding, coiled-coil and Rab domains suggest that Rab46 will be important for coupling rapid responses to signalling in many cell types. The molecular...

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Autores principales:	Lucia Pedicini, Sabina D. Wiktor, Katie J. Simmons, Ashley Money, Lynn McKeown
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c6b1162121634a90b1082cf02940df59
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spelling	oai:doaj.org-article:c6b1162121634a90b1082cf02940df592021-12-02T10:54:30ZAffinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells10.1038/s41598-021-83560-y2045-2322https://doaj.org/article/c6b1162121634a90b1082cf02940df592021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83560-yhttps://doaj.org/toc/2045-2322Abstract Rab46 is a novel Ca2+-sensing Rab GTPase shown to have important functions in endothelial and immune cells. The presence of functional Ca2+-binding, coiled-coil and Rab domains suggest that Rab46 will be important for coupling rapid responses to signalling in many cell types. The molecular mechanisms underlying Rab46 function are currently unknown. Here we provide the first resource for studying Rab46 interacting proteins. Using liquid chromatography tandem mass spectrometry (LC–MS/MS) to identify affinity purified proteins that bind to constitutively active GFP-Rab46 or inactive GFP-Rab46 expressed in endothelial cells, we have revealed 922 peptides that interact with either the GTP-bound Rab46 or GDP-bound Rab46. To identify proteins that could be potential Rab46 effectors we performed further comparative analyses between nucleotide-locked Rab46 proteins and identified 29 candidate effector proteins. Importantly, through biochemical and imaging approaches we have validated two potential effector proteins; dynein and the Na2+/ K+ ATPase subunit alpha 1 (ATP1α1). Hence, our use of affinity purification and LC–MS/MS to identify Rab46 neighbouring proteins provides a valuable resource for detecting Rab46 effector proteins and analysing Rab46 functions.Lucia PediciniSabina D. WiktorKatie J. SimmonsAshley MoneyLynn McKeownNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucia Pedicini Sabina D. Wiktor Katie J. Simmons Ashley Money Lynn McKeown Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells
description	Abstract Rab46 is a novel Ca2+-sensing Rab GTPase shown to have important functions in endothelial and immune cells. The presence of functional Ca2+-binding, coiled-coil and Rab domains suggest that Rab46 will be important for coupling rapid responses to signalling in many cell types. The molecular mechanisms underlying Rab46 function are currently unknown. Here we provide the first resource for studying Rab46 interacting proteins. Using liquid chromatography tandem mass spectrometry (LC–MS/MS) to identify affinity purified proteins that bind to constitutively active GFP-Rab46 or inactive GFP-Rab46 expressed in endothelial cells, we have revealed 922 peptides that interact with either the GTP-bound Rab46 or GDP-bound Rab46. To identify proteins that could be potential Rab46 effectors we performed further comparative analyses between nucleotide-locked Rab46 proteins and identified 29 candidate effector proteins. Importantly, through biochemical and imaging approaches we have validated two potential effector proteins; dynein and the Na2+/ K+ ATPase subunit alpha 1 (ATP1α1). Hence, our use of affinity purification and LC–MS/MS to identify Rab46 neighbouring proteins provides a valuable resource for detecting Rab46 effector proteins and analysing Rab46 functions.
format	article
author	Lucia Pedicini Sabina D. Wiktor Katie J. Simmons Ashley Money Lynn McKeown
author_facet	Lucia Pedicini Sabina D. Wiktor Katie J. Simmons Ashley Money Lynn McKeown
author_sort	Lucia Pedicini
title	Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells
title_short	Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells
title_full	Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells
title_fullStr	Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells
title_full_unstemmed	Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells
title_sort	affinity-based proteomics reveals novel binding partners for rab46 in endothelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c6b1162121634a90b1082cf02940df59
work_keys_str_mv	AT luciapedicini affinitybasedproteomicsrevealsnovelbindingpartnersforrab46inendothelialcells AT sabinadwiktor affinitybasedproteomicsrevealsnovelbindingpartnersforrab46inendothelialcells AT katiejsimmons affinitybasedproteomicsrevealsnovelbindingpartnersforrab46inendothelialcells AT ashleymoney affinitybasedproteomicsrevealsnovelbindingpartnersforrab46inendothelialcells AT lynnmckeown affinitybasedproteomicsrevealsnovelbindingpartnersforrab46inendothelialcells
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Affinity-based proteomics reveals novel binding partners for Rab46 in endothelial cells

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