The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.

Small non-coding regulatory RNAs (sRNAs) post-transcriptionally affect multiple phenotypes in prokaryotes and eukaryotes, yet most of the underlying regulatory mechanisms and the nature of the target mRNAs remain unclear. Here we report the identification and functional analysis of the novel cis-enc...

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Autores principales:	Christian G Ramos, Paulo J P da Costa, Gerd Döring, Jorge H Leitão
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c6b7b8e34a2d4441aaa8e6ce89fc8bb0
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spelling	oai:doaj.org-article:c6b7b8e34a2d4441aaa8e6ce89fc8bb02021-11-18T08:11:35ZThe novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.1932-620310.1371/journal.pone.0047896https://doaj.org/article/c6b7b8e34a2d4441aaa8e6ce89fc8bb02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23082228/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Small non-coding regulatory RNAs (sRNAs) post-transcriptionally affect multiple phenotypes in prokaryotes and eukaryotes, yet most of the underlying regulatory mechanisms and the nature of the target mRNAs remain unclear. Here we report the identification and functional analysis of the novel cis-encoded sRNA h2cR, from the human opportunistic pathogen Burkholderia cenocepacia J2315. The sRNA was found to negatively regulate the hfq2 mRNA, through binding to part of the 5'-UTR region of the hfq2 mRNA, resulting in accelerated hfq2 mRNA decay and reduced protein levels in exponentially growing cells. Both the h2cR transcript and the hfq2 mRNA are stabilized by the other B. cenocepacia RNA chaperone, Hfq. Infection experiments using the nematode Caenorhabditis elegans revealed that down-regulation of Hfq2 by h2cR decreases the B. cenocepacia ability to colonize and persist within the nematode, suggesting a role for h2cR on bacterial persistence in the host.Christian G RamosPaulo J P da CostaGerd DöringJorge H LeitãoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47896 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian G Ramos Paulo J P da Costa Gerd Döring Jorge H Leitão The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
description	Small non-coding regulatory RNAs (sRNAs) post-transcriptionally affect multiple phenotypes in prokaryotes and eukaryotes, yet most of the underlying regulatory mechanisms and the nature of the target mRNAs remain unclear. Here we report the identification and functional analysis of the novel cis-encoded sRNA h2cR, from the human opportunistic pathogen Burkholderia cenocepacia J2315. The sRNA was found to negatively regulate the hfq2 mRNA, through binding to part of the 5'-UTR region of the hfq2 mRNA, resulting in accelerated hfq2 mRNA decay and reduced protein levels in exponentially growing cells. Both the h2cR transcript and the hfq2 mRNA are stabilized by the other B. cenocepacia RNA chaperone, Hfq. Infection experiments using the nematode Caenorhabditis elegans revealed that down-regulation of Hfq2 by h2cR decreases the B. cenocepacia ability to colonize and persist within the nematode, suggesting a role for h2cR on bacterial persistence in the host.
format	article
author	Christian G Ramos Paulo J P da Costa Gerd Döring Jorge H Leitão
author_facet	Christian G Ramos Paulo J P da Costa Gerd Döring Jorge H Leitão
author_sort	Christian G Ramos
title	The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_short	The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_full	The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_fullStr	The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_full_unstemmed	The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_sort	novel cis-encoded small rna h2cr is a negative regulator of hfq2 in burkholderia cenocepacia.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/c6b7b8e34a2d4441aaa8e6ce89fc8bb0
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The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.

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