The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.

Small non-coding regulatory RNAs (sRNAs) post-transcriptionally affect multiple phenotypes in prokaryotes and eukaryotes, yet most of the underlying regulatory mechanisms and the nature of the target mRNAs remain unclear. Here we report the identification and functional analysis of the novel cis-enc...

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Autores principales: Christian G Ramos, Paulo J P da Costa, Gerd Döring, Jorge H Leitão
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Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/c6b7b8e34a2d4441aaa8e6ce89fc8bb0
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spelling oai:doaj.org-article:c6b7b8e34a2d4441aaa8e6ce89fc8bb02021-11-18T08:11:35ZThe novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.1932-620310.1371/journal.pone.0047896https://doaj.org/article/c6b7b8e34a2d4441aaa8e6ce89fc8bb02012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23082228/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Small non-coding regulatory RNAs (sRNAs) post-transcriptionally affect multiple phenotypes in prokaryotes and eukaryotes, yet most of the underlying regulatory mechanisms and the nature of the target mRNAs remain unclear. Here we report the identification and functional analysis of the novel cis-encoded sRNA h2cR, from the human opportunistic pathogen Burkholderia cenocepacia J2315. The sRNA was found to negatively regulate the hfq2 mRNA, through binding to part of the 5'-UTR region of the hfq2 mRNA, resulting in accelerated hfq2 mRNA decay and reduced protein levels in exponentially growing cells. Both the h2cR transcript and the hfq2 mRNA are stabilized by the other B. cenocepacia RNA chaperone, Hfq. Infection experiments using the nematode Caenorhabditis elegans revealed that down-regulation of Hfq2 by h2cR decreases the B. cenocepacia ability to colonize and persist within the nematode, suggesting a role for h2cR on bacterial persistence in the host.Christian G RamosPaulo J P da CostaGerd DöringJorge H LeitãoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e47896 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Christian G Ramos
Paulo J P da Costa
Gerd Döring
Jorge H Leitão
The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
description Small non-coding regulatory RNAs (sRNAs) post-transcriptionally affect multiple phenotypes in prokaryotes and eukaryotes, yet most of the underlying regulatory mechanisms and the nature of the target mRNAs remain unclear. Here we report the identification and functional analysis of the novel cis-encoded sRNA h2cR, from the human opportunistic pathogen Burkholderia cenocepacia J2315. The sRNA was found to negatively regulate the hfq2 mRNA, through binding to part of the 5'-UTR region of the hfq2 mRNA, resulting in accelerated hfq2 mRNA decay and reduced protein levels in exponentially growing cells. Both the h2cR transcript and the hfq2 mRNA are stabilized by the other B. cenocepacia RNA chaperone, Hfq. Infection experiments using the nematode Caenorhabditis elegans revealed that down-regulation of Hfq2 by h2cR decreases the B. cenocepacia ability to colonize and persist within the nematode, suggesting a role for h2cR on bacterial persistence in the host.
format article
author Christian G Ramos
Paulo J P da Costa
Gerd Döring
Jorge H Leitão
author_facet Christian G Ramos
Paulo J P da Costa
Gerd Döring
Jorge H Leitão
author_sort Christian G Ramos
title The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_short The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_full The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_fullStr The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_full_unstemmed The novel cis-encoded small RNA h2cR is a negative regulator of hfq2 in Burkholderia cenocepacia.
title_sort novel cis-encoded small rna h2cr is a negative regulator of hfq2 in burkholderia cenocepacia.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/c6b7b8e34a2d4441aaa8e6ce89fc8bb0
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