Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain

Yaswanth Kuthati,1 Prabhakar Busa,2 Venkata Naga Goutham Davuluri,3 Chih Shung Wong1 1Department of Anesthesiology, Cathy General Hospital, Taipei, Taiwan; 2Department of Life Sciences, National Dong Hwa University, Hualien, Taiwan; 3Department of Microbiology and Immunology, College of Medicine, Na...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kuthati Y, Busa P, Goutham Davuluri VN, Wong CS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	allodynia monps reactive oxygen species and neuropathic pain Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c6b85f07dada4c34be545c5f7a314679
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:c6b85f07dada4c34be545c5f7a314679
record_format	dspace
spelling	oai:doaj.org-article:c6b85f07dada4c34be545c5f7a3146792021-12-02T06:56:50ZManganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain1178-2013https://doaj.org/article/c6b85f07dada4c34be545c5f7a3146792019-12-01T00:00:00Zhttps://www.dovepress.com/manganese-oxide-nanozymes-ameliorate-mechanical-allodynia-in-a-rat-mod-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yaswanth Kuthati,1 Prabhakar Busa,2 Venkata Naga Goutham Davuluri,3 Chih Shung Wong1 1Department of Anesthesiology, Cathy General Hospital, Taipei, Taiwan; 2Department of Life Sciences, National Dong Hwa University, Hualien, Taiwan; 3Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanCorrespondence: Chih Shung WongDepartment of Anesthesiology, Cathay General Hospital, #280, Renai Road, Section 4, Taipei, TaiwanTel +886 2 27082121 Ext 3510Email w82556@gmail.comBackground: Reactive oxygen species (ROS) induced oxidative stress is linked to numerous neurological diseases, including neuropathic pain. Natural ROS scavenging enzymes like superoxide dismutase (SOD) and catalase have been found to be efficient in alleviating neuropathic pain. However, their sensitivity towards extreme pH and a short half-life limit their efficacy in vivo. Manganese oxide nanoparticles (MONPs) are recently known to possess ROS scavenging properties. In this study, MONPs were examined for their therapeutic effect on neuropathic pain.Methods: The MONPs were synthesized by a hydrothermal method. The synthesized MONPs were characterized by UV/Vis, TEM, SEM, FTIR, NTA and XRD. The biocompatibility of the nanoparticles is evaluated in neural cells using LDH assay. MONPs were evaluated for their antioxidant activity by DPPH assay. In addition, in vitro ROS scavenging properties were examined in bone marrow-derived macrophage (BMDM) cells using 2ʹ,7ʹ-dichlorofluorescin diacetate (DCFDA) assay. To evaluate the in vivo efficacy of nanoparticles, neuropathic pain was induced in Wistar rats by partial sciatic nerve transection (PSNT). On post-transection days 14 to 18, rats were intrathecally injected with MONPs and paw withdrawal threshold was measured. The spinal cords were collected and processed for Western blotting and histological analysis.Results: The synthesized MONPs were biocompatible and showed effective antioxidant activity against DPPH free radical scavenging. Further, the nanoparticles scavenged ROS efficiently in vitro in BMDM and their intrathecal administration significantly reduced mechanical allodynia as well as the expression of cyclooxygenase-2 (COX-2), an important mediator of chronic and inflammatory pain in the spinal dorsal horns of PSNT rats.Conclusion: As ROS play a significant role in neuropathic pain, we expect that MONPs could be a promising tool for the treatment of various inflammatory diseases and might also serve as a potential nanocarrier for the delivery of analgesics.Keywords: allodynia, MONPs, reactive oxygen species and neuropathic painKuthati YBusa PGoutham Davuluri VNWong CSDove Medical Pressarticleallodyniamonpsreactive oxygen species and neuropathic painMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 10105-10117 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	allodynia monps reactive oxygen species and neuropathic pain Medicine (General) R5-920
spellingShingle	allodynia monps reactive oxygen species and neuropathic pain Medicine (General) R5-920 Kuthati Y Busa P Goutham Davuluri VN Wong CS Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
description	Yaswanth Kuthati,1 Prabhakar Busa,2 Venkata Naga Goutham Davuluri,3 Chih Shung Wong1 1Department of Anesthesiology, Cathy General Hospital, Taipei, Taiwan; 2Department of Life Sciences, National Dong Hwa University, Hualien, Taiwan; 3Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University, Tainan, TaiwanCorrespondence: Chih Shung WongDepartment of Anesthesiology, Cathay General Hospital, #280, Renai Road, Section 4, Taipei, TaiwanTel +886 2 27082121 Ext 3510Email w82556@gmail.comBackground: Reactive oxygen species (ROS) induced oxidative stress is linked to numerous neurological diseases, including neuropathic pain. Natural ROS scavenging enzymes like superoxide dismutase (SOD) and catalase have been found to be efficient in alleviating neuropathic pain. However, their sensitivity towards extreme pH and a short half-life limit their efficacy in vivo. Manganese oxide nanoparticles (MONPs) are recently known to possess ROS scavenging properties. In this study, MONPs were examined for their therapeutic effect on neuropathic pain.Methods: The MONPs were synthesized by a hydrothermal method. The synthesized MONPs were characterized by UV/Vis, TEM, SEM, FTIR, NTA and XRD. The biocompatibility of the nanoparticles is evaluated in neural cells using LDH assay. MONPs were evaluated for their antioxidant activity by DPPH assay. In addition, in vitro ROS scavenging properties were examined in bone marrow-derived macrophage (BMDM) cells using 2ʹ,7ʹ-dichlorofluorescin diacetate (DCFDA) assay. To evaluate the in vivo efficacy of nanoparticles, neuropathic pain was induced in Wistar rats by partial sciatic nerve transection (PSNT). On post-transection days 14 to 18, rats were intrathecally injected with MONPs and paw withdrawal threshold was measured. The spinal cords were collected and processed for Western blotting and histological analysis.Results: The synthesized MONPs were biocompatible and showed effective antioxidant activity against DPPH free radical scavenging. Further, the nanoparticles scavenged ROS efficiently in vitro in BMDM and their intrathecal administration significantly reduced mechanical allodynia as well as the expression of cyclooxygenase-2 (COX-2), an important mediator of chronic and inflammatory pain in the spinal dorsal horns of PSNT rats.Conclusion: As ROS play a significant role in neuropathic pain, we expect that MONPs could be a promising tool for the treatment of various inflammatory diseases and might also serve as a potential nanocarrier for the delivery of analgesics.Keywords: allodynia, MONPs, reactive oxygen species and neuropathic pain
format	article
author	Kuthati Y Busa P Goutham Davuluri VN Wong CS
author_facet	Kuthati Y Busa P Goutham Davuluri VN Wong CS
author_sort	Kuthati Y
title	Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
title_short	Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
title_full	Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
title_fullStr	Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
title_full_unstemmed	Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain
title_sort	manganese oxide nanozymes ameliorate mechanical allodynia in a rat model of partial sciatic nerve-transection induced neuropathic pain
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/c6b85f07dada4c34be545c5f7a314679
work_keys_str_mv	AT kuthatiy manganeseoxidenanozymesamelioratemechanicalallodyniainaratmodelofpartialsciaticnervetransectioninducedneuropathicpain AT busap manganeseoxidenanozymesamelioratemechanicalallodyniainaratmodelofpartialsciaticnervetransectioninducedneuropathicpain AT gouthamdavulurivn manganeseoxidenanozymesamelioratemechanicalallodyniainaratmodelofpartialsciaticnervetransectioninducedneuropathicpain AT wongcs manganeseoxidenanozymesamelioratemechanicalallodyniainaratmodelofpartialsciaticnervetransectioninducedneuropathicpain
_version_	1718399660464275456

Manganese Oxide Nanozymes Ameliorate Mechanical Allodynia in a Rat Model of Partial Sciatic Nerve-Transection Induced Neuropathic Pain

Ejemplares similares