Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions

While some genetically modified (GM) plants have been targeted to confer tolerance to abiotic stressors, transgenes are impacted by abiotic stressors, causing adverse effects on plant physiology and yield. However, routine safety analyses do not assess the response of GM plants under different envir...

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Autores principales:	Rafael Fonseca Benevenuto, Caroline Bedin Zanatta, Miguel Pedro Guerra, Rubens Onofre Nodari, Sarah Z. Agapito-Tenfen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	energy cost budget EPSPS fitness cost glyphosate <i>Glycine max</i> abiotic stress Botany QK1-989
Acceso en línea:	https://doaj.org/article/c6bb79a6b3d446218c13e86b3170b363
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id	oai:doaj.org-article:c6bb79a6b3d446218c13e86b3170b363
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spelling	oai:doaj.org-article:c6bb79a6b3d446218c13e86b3170b3632021-11-25T18:46:05ZProteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions10.3390/plants101123812223-7747https://doaj.org/article/c6bb79a6b3d446218c13e86b3170b3632021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2381https://doaj.org/toc/2223-7747While some genetically modified (GM) plants have been targeted to confer tolerance to abiotic stressors, transgenes are impacted by abiotic stressors, causing adverse effects on plant physiology and yield. However, routine safety analyses do not assess the response of GM plants under different environmental stress conditions. In the context of climate change, the combination of abiotic stressors is a reality in agroecosystems. Therefore, the aim of this study was to analyze the metabolic cost by assessing the proteomic profiles of GM soybean varieties under glyphosate spraying and water deficit conditions compared to their non-transgenic conventional counterparts. We found evidence of cumulative adverse effects that resulted in the reduction of enzymes involved in carbohydrate metabolism, along with the expression of amino acids and nitrogen metabolic enzymes. Ribosomal metabolism was significantly enriched, particularly the protein families associated with ribosomal complexes L5 and L18. The interaction network map showed that the affected module representing the ribosome pathway interacts strongly with other important proteins, such as the chloro-plastic gamma ATP synthase subunit. Combined, these findings provide clear evidence for increasing the metabolic costs of GM soybean plants in response to the accumulation of stress factors. First, alterations in the ribosome pathway indicate that the GM plant itself carries a metabolic burden associated with the biosynthesis of proteins as effects of genetic transformation. GM plants also showed an imbalance in energy demand and production under controlled conditions, which was increased under drought conditions. Identifying the consequences of altered metabolism related to the interaction between plant transgene stress responses allows us to understand the possible effects on the ecology and evolution of plants in the medium and long term and the potential interactions with other organisms when these organisms are released in the environment.Rafael Fonseca BenevenutoCaroline Bedin ZanattaMiguel Pedro GuerraRubens Onofre NodariSarah Z. Agapito-TenfenMDPI AGarticleenergy cost budgetEPSPSfitness costglyphosate<i>Glycine max</i>abiotic stressBotanyQK1-989ENPlants, Vol 10, Iss 2381, p 2381 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	energy cost budget EPSPS fitness cost glyphosate <i>Glycine max</i> abiotic stress Botany QK1-989
spellingShingle	energy cost budget EPSPS fitness cost glyphosate <i>Glycine max</i> abiotic stress Botany QK1-989 Rafael Fonseca Benevenuto Caroline Bedin Zanatta Miguel Pedro Guerra Rubens Onofre Nodari Sarah Z. Agapito-Tenfen Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions
description	While some genetically modified (GM) plants have been targeted to confer tolerance to abiotic stressors, transgenes are impacted by abiotic stressors, causing adverse effects on plant physiology and yield. However, routine safety analyses do not assess the response of GM plants under different environmental stress conditions. In the context of climate change, the combination of abiotic stressors is a reality in agroecosystems. Therefore, the aim of this study was to analyze the metabolic cost by assessing the proteomic profiles of GM soybean varieties under glyphosate spraying and water deficit conditions compared to their non-transgenic conventional counterparts. We found evidence of cumulative adverse effects that resulted in the reduction of enzymes involved in carbohydrate metabolism, along with the expression of amino acids and nitrogen metabolic enzymes. Ribosomal metabolism was significantly enriched, particularly the protein families associated with ribosomal complexes L5 and L18. The interaction network map showed that the affected module representing the ribosome pathway interacts strongly with other important proteins, such as the chloro-plastic gamma ATP synthase subunit. Combined, these findings provide clear evidence for increasing the metabolic costs of GM soybean plants in response to the accumulation of stress factors. First, alterations in the ribosome pathway indicate that the GM plant itself carries a metabolic burden associated with the biosynthesis of proteins as effects of genetic transformation. GM plants also showed an imbalance in energy demand and production under controlled conditions, which was increased under drought conditions. Identifying the consequences of altered metabolism related to the interaction between plant transgene stress responses allows us to understand the possible effects on the ecology and evolution of plants in the medium and long term and the potential interactions with other organisms when these organisms are released in the environment.
format	article
author	Rafael Fonseca Benevenuto Caroline Bedin Zanatta Miguel Pedro Guerra Rubens Onofre Nodari Sarah Z. Agapito-Tenfen
author_facet	Rafael Fonseca Benevenuto Caroline Bedin Zanatta Miguel Pedro Guerra Rubens Onofre Nodari Sarah Z. Agapito-Tenfen
author_sort	Rafael Fonseca Benevenuto
title	Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions
title_short	Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions
title_full	Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions
title_fullStr	Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions
title_full_unstemmed	Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions
title_sort	proteomic profile of glyphosate-resistant soybean under combined herbicide and drought stress conditions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c6bb79a6b3d446218c13e86b3170b363
work_keys_str_mv	AT rafaelfonsecabenevenuto proteomicprofileofglyphosateresistantsoybeanundercombinedherbicideanddroughtstressconditions AT carolinebedinzanatta proteomicprofileofglyphosateresistantsoybeanundercombinedherbicideanddroughtstressconditions AT miguelpedroguerra proteomicprofileofglyphosateresistantsoybeanundercombinedherbicideanddroughtstressconditions AT rubensonofrenodari proteomicprofileofglyphosateresistantsoybeanundercombinedherbicideanddroughtstressconditions AT sarahzagapitotenfen proteomicprofileofglyphosateresistantsoybeanundercombinedherbicideanddroughtstressconditions
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Proteomic Profile of Glyphosate-Resistant Soybean under Combined Herbicide and Drought Stress Conditions

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