The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions

Xuan Li,1 Chi-Hin Wong,2 Tsz-Wing Ng,2 Cheng-Fei Zhang,1 Ken Cham-Fai Leung,2 Lijian Jin1 1Faculty of Dentistry, The University of Hong Kong, 2Department of Chemistry, Institute of Creativity and Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Ho...

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Autores principales:	Li X, Wong CH, Ng TW, Zhang CF, Leung KC, Jin LJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	mesoporous silica nanoparticles morphology chlorhexidine imaging biofilms Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c6c29fbc4da24203bba3dcae79cc83f5
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spelling	oai:doaj.org-article:c6c29fbc4da24203bba3dcae79cc83f52021-12-02T04:23:13ZThe spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions1178-2013https://doaj.org/article/c6c29fbc4da24203bba3dcae79cc83f52016-05-01T00:00:00Zhttps://www.dovepress.com/the-spherical-nanoparticle-encapsulated-chlorhexidine-enhances-anti-bi-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xuan Li,1 Chi-Hin Wong,2 Tsz-Wing Ng,2 Cheng-Fei Zhang,1 Ken Cham-Fai Leung,2 Lijian Jin1 1Faculty of Dentistry, The University of Hong Kong, 2Department of Chemistry, Institute of Creativity and Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong SAR, People’s Republic of China Abstract: We reported two forms (sphere and wire) of newly fabricated chlorhexidine (CHX)-loaded mesoporous silica nanoparticles (MSNs), and investigated their releasing capacities and anti-biofilm efficiencies. The interactions of the blank MSNs with planktonic oral microorganisms were assessed by field emission scanning electron microscopy. The anti-biofilm effects of the two forms of nanoparticle-encapsulated CHX were examined by 2,3-bis (2-methoxy- 4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide. The profiles of biofilm penetration were analyzed by fluorescent-labeled MSNs using confocal microscopy and ImageJ. The spherical MSNs with an average diameter of 265 nm exhibited a larger surface area and faster CHX-releasing rate than the MSN wires. The field emission scanning electron microscopy images showed that both shaped MSNs enabled to attach and further fuse with the surfaces of testing microbes. Meanwhile, the nanoparticle-encapsulated CHX could enhance the anti-biofilm efficiency with reference to its free form. Notably, the spherical nanoparticle-encapsulated CHX presented with a greater anti-biofilm capacity than the wire nanoparticle-encapsulated CHX, partly due to their difference in physical property. Furthermore, the relatively even distribution and homogeneous dispersion of spherical MSNs observed in confocal images may account for the enhanced penetration of spherical nanoparticle-encapsulated CHX into the microbial biofilms and resultant anti-biofilm effects. These findings reveal that the spherical nanoparticle-encapsulated CHX could preferably enhance its anti-biofilm efficiency through an effective releasing mode and close interactions with microbes. Keywords: mesoporous silica nanoparticles, morphology, chlorhexidine, imaging, biofilmsLi XWong CHNg TWZhang CFLeung KCJin LJDove Medical Pressarticlemesoporous silica nanoparticlesmorphologychlorhexidineimagingbiofilmsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss default, Pp 2471-2480 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	mesoporous silica nanoparticles morphology chlorhexidine imaging biofilms Medicine (General) R5-920
spellingShingle	mesoporous silica nanoparticles morphology chlorhexidine imaging biofilms Medicine (General) R5-920 Li X Wong CH Ng TW Zhang CF Leung KC Jin LJ The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
description	Xuan Li,1 Chi-Hin Wong,2 Tsz-Wing Ng,2 Cheng-Fei Zhang,1 Ken Cham-Fai Leung,2 Lijian Jin1 1Faculty of Dentistry, The University of Hong Kong, 2Department of Chemistry, Institute of Creativity and Partner State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong SAR, People’s Republic of China Abstract: We reported two forms (sphere and wire) of newly fabricated chlorhexidine (CHX)-loaded mesoporous silica nanoparticles (MSNs), and investigated their releasing capacities and anti-biofilm efficiencies. The interactions of the blank MSNs with planktonic oral microorganisms were assessed by field emission scanning electron microscopy. The anti-biofilm effects of the two forms of nanoparticle-encapsulated CHX were examined by 2,3-bis (2-methoxy- 4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide. The profiles of biofilm penetration were analyzed by fluorescent-labeled MSNs using confocal microscopy and ImageJ. The spherical MSNs with an average diameter of 265 nm exhibited a larger surface area and faster CHX-releasing rate than the MSN wires. The field emission scanning electron microscopy images showed that both shaped MSNs enabled to attach and further fuse with the surfaces of testing microbes. Meanwhile, the nanoparticle-encapsulated CHX could enhance the anti-biofilm efficiency with reference to its free form. Notably, the spherical nanoparticle-encapsulated CHX presented with a greater anti-biofilm capacity than the wire nanoparticle-encapsulated CHX, partly due to their difference in physical property. Furthermore, the relatively even distribution and homogeneous dispersion of spherical MSNs observed in confocal images may account for the enhanced penetration of spherical nanoparticle-encapsulated CHX into the microbial biofilms and resultant anti-biofilm effects. These findings reveal that the spherical nanoparticle-encapsulated CHX could preferably enhance its anti-biofilm efficiency through an effective releasing mode and close interactions with microbes. Keywords: mesoporous silica nanoparticles, morphology, chlorhexidine, imaging, biofilms
format	article
author	Li X Wong CH Ng TW Zhang CF Leung KC Jin LJ
author_facet	Li X Wong CH Ng TW Zhang CF Leung KC Jin LJ
author_sort	Li X
title	The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
title_short	The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
title_full	The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
title_fullStr	The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
title_full_unstemmed	The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
title_sort	spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/c6c29fbc4da24203bba3dcae79cc83f5
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The spherical nanoparticle-encapsulated chlorhexidine enhances anti-biofilm efficiency through an effective releasing mode and close microbial interactions

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