TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS

Abstract. Present study deals with size measurements of telomeric DNA from the human peripheral mononuclear immune cells in rheumatoid arthritis (RA). A method for measuring the relative telomere length by in situ hybridization followed by flow cytometric analysis (flow-FISH) was used. Relative telo...

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Autores principales:	V. I. Borisov, V. S. Kozhevnikov, V. V. Seniukov, A. E. Sizikov, L. P. Konenkova, O. V. Gerzog, V. A. Kozlov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	telomere flow cytometry rheumatoid arthritis Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c6cb505386004e2e81e9329bd80870c4
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spelling	oai:doaj.org-article:c6cb505386004e2e81e9329bd80870c42021-11-18T08:03:41ZTELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS1563-06252313-741X10.15789/1563-0625-2006-1-87-90https://doaj.org/article/c6cb505386004e2e81e9329bd80870c42014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/443https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Present study deals with size measurements of telomeric DNA from the human peripheral mononuclear immune cells in rheumatoid arthritis (RA). A method for measuring the relative telomere length by in situ hybridization followed by flow cytometric analysis (flow-FISH) was used. Relative telomere length (RTL) in monocytes was estimated as mean fluorescence intensity (MFI) of test cells divided by MFI values of internal control cells. Hybridization conditions for analysis of telomere length in monocytes have been optimized in advance. It has been shown that RTL of monocytes was significantly lower in RA patients compared to donors. Significant differences in telomere length of monocytes between RA patients and donors were revealed for the young persons under 30 years old. The findings obtained may be considered as an additional argument confirming the hypothesis on genetic defects of hematopoietic stem cells determining RA development.V. I. BorisovV. S. KozhevnikovV. V. SeniukovA. E. SizikovL. P. KonenkovaO. V. GerzogV. A. KozlovSPb RAACIarticletelomereflow cytometryrheumatoid arthritisImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 8, Iss 1, Pp 87-90 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	telomere flow cytometry rheumatoid arthritis Immunologic diseases. Allergy RC581-607
spellingShingle	telomere flow cytometry rheumatoid arthritis Immunologic diseases. Allergy RC581-607 V. I. Borisov V. S. Kozhevnikov V. V. Seniukov A. E. Sizikov L. P. Konenkova O. V. Gerzog V. A. Kozlov TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS
description	Abstract. Present study deals with size measurements of telomeric DNA from the human peripheral mononuclear immune cells in rheumatoid arthritis (RA). A method for measuring the relative telomere length by in situ hybridization followed by flow cytometric analysis (flow-FISH) was used. Relative telomere length (RTL) in monocytes was estimated as mean fluorescence intensity (MFI) of test cells divided by MFI values of internal control cells. Hybridization conditions for analysis of telomere length in monocytes have been optimized in advance. It has been shown that RTL of monocytes was significantly lower in RA patients compared to donors. Significant differences in telomere length of monocytes between RA patients and donors were revealed for the young persons under 30 years old. The findings obtained may be considered as an additional argument confirming the hypothesis on genetic defects of hematopoietic stem cells determining RA development.
format	article
author	V. I. Borisov V. S. Kozhevnikov V. V. Seniukov A. E. Sizikov L. P. Konenkova O. V. Gerzog V. A. Kozlov
author_facet	V. I. Borisov V. S. Kozhevnikov V. V. Seniukov A. E. Sizikov L. P. Konenkova O. V. Gerzog V. A. Kozlov
author_sort	V. I. Borisov
title	TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS
title_short	TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS
title_full	TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS
title_fullStr	TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS
title_full_unstemmed	TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS
title_sort	telomere shortening in monocytes of the patients with rheumatoid arthritis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/c6cb505386004e2e81e9329bd80870c4
work_keys_str_mv	AT viborisov telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis AT vskozhevnikov telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis AT vvseniukov telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis AT aesizikov telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis AT lpkonenkova telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis AT ovgerzog telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis AT vakozlov telomereshorteninginmonocytesofthepatientswithrheumatoidarthritis
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TELOMERE SHORTENING IN MONOCYTES OF THE PATIENTS WITH RHEUMATOID ARTHRITIS

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