Impact of aging on gene expression response to x-ray irradiation using mouse blood

Abstract As a radiation biodosimetry tool, gene expression profiling is being developed using mouse and human peripheral blood models. The impact of dose, dose-rate, and radiation quality has been studied with the goal of predicting radiological tissue injury. In this study, we determined the impact...

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Autores principales:	Constantinos G. Broustas, Axel J. Duval, Sally A. Amundson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c6d13f617c18403e8d878e2dccde2a78
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spelling	oai:doaj.org-article:c6d13f617c18403e8d878e2dccde2a782021-12-02T17:15:32ZImpact of aging on gene expression response to x-ray irradiation using mouse blood10.1038/s41598-021-89682-72045-2322https://doaj.org/article/c6d13f617c18403e8d878e2dccde2a782021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89682-7https://doaj.org/toc/2045-2322Abstract As a radiation biodosimetry tool, gene expression profiling is being developed using mouse and human peripheral blood models. The impact of dose, dose-rate, and radiation quality has been studied with the goal of predicting radiological tissue injury. In this study, we determined the impact of aging on the gene expression profile of blood from mice exposed to radiation. Young (2 mo) and old (21 mo) male mice were irradiated with 4 Gy x-rays, total RNA was isolated from whole blood 24 h later, and subjected to whole genome microarray analysis. Pathway analysis of differentially expressed genes revealed young mice responded to x-ray exposure by significantly upregulating pathways involved in apoptosis and phagocytosis, a process that eliminates apoptotic cells and preserves tissue homeostasis. In contrast, the functional annotation of senescence was overrepresented among differentially expressed genes from irradiated old mice without enrichment of phagocytosis pathways. Pathways associated with hematologic malignancies were enriched in irradiated old mice compared with irradiated young mice. The fibroblast growth factor signaling pathway was underrepresented in older mice under basal conditions. Similarly, brain-related functions were underrepresented in unirradiated old mice. Thus, age-dependent gene expression differences should be considered when developing gene signatures for use in radiation biodosimetry.Constantinos G. BroustasAxel J. DuvalSally A. AmundsonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Constantinos G. Broustas Axel J. Duval Sally A. Amundson Impact of aging on gene expression response to x-ray irradiation using mouse blood
description	Abstract As a radiation biodosimetry tool, gene expression profiling is being developed using mouse and human peripheral blood models. The impact of dose, dose-rate, and radiation quality has been studied with the goal of predicting radiological tissue injury. In this study, we determined the impact of aging on the gene expression profile of blood from mice exposed to radiation. Young (2 mo) and old (21 mo) male mice were irradiated with 4 Gy x-rays, total RNA was isolated from whole blood 24 h later, and subjected to whole genome microarray analysis. Pathway analysis of differentially expressed genes revealed young mice responded to x-ray exposure by significantly upregulating pathways involved in apoptosis and phagocytosis, a process that eliminates apoptotic cells and preserves tissue homeostasis. In contrast, the functional annotation of senescence was overrepresented among differentially expressed genes from irradiated old mice without enrichment of phagocytosis pathways. Pathways associated with hematologic malignancies were enriched in irradiated old mice compared with irradiated young mice. The fibroblast growth factor signaling pathway was underrepresented in older mice under basal conditions. Similarly, brain-related functions were underrepresented in unirradiated old mice. Thus, age-dependent gene expression differences should be considered when developing gene signatures for use in radiation biodosimetry.
format	article
author	Constantinos G. Broustas Axel J. Duval Sally A. Amundson
author_facet	Constantinos G. Broustas Axel J. Duval Sally A. Amundson
author_sort	Constantinos G. Broustas
title	Impact of aging on gene expression response to x-ray irradiation using mouse blood
title_short	Impact of aging on gene expression response to x-ray irradiation using mouse blood
title_full	Impact of aging on gene expression response to x-ray irradiation using mouse blood
title_fullStr	Impact of aging on gene expression response to x-ray irradiation using mouse blood
title_full_unstemmed	Impact of aging on gene expression response to x-ray irradiation using mouse blood
title_sort	impact of aging on gene expression response to x-ray irradiation using mouse blood
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c6d13f617c18403e8d878e2dccde2a78
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Impact of aging on gene expression response to x-ray irradiation using mouse blood

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