EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA

Abstract. Samples of renal cell carcinoma (RCC) and adjacent normal tissues were studied by means of immunoblotting, using monoclonal antibodies [(MKA 1, 1F3-2D4, IgG1 class), MKA 2H6, IgG1 class] against membrane proteins of HEp-2 cells (larynx cancer). Strong reactivity of MKA 1 was revealed with...

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Autores principales:	T. S. Bobrova, Yu. V. Chuev
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	renal cell carcinomas monoclonal antibodies immunoblotting Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/c6ea110974204b81b80890024ff4b26d
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spelling	oai:doaj.org-article:c6ea110974204b81b80890024ff4b26d2021-11-18T08:03:38ZEXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA1563-06252313-741X10.15789/1563-0625-2008-2-3-209-214https://doaj.org/article/c6ea110974204b81b80890024ff4b26d2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/127https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Samples of renal cell carcinoma (RCC) and adjacent normal tissues were studied by means of immunoblotting, using monoclonal antibodies [(MKA 1, 1F3-2D4, IgG1 class), MKA 2H6, IgG1 class] against membrane proteins of HEp-2 cells (larynx cancer). Strong reactivity of MKA 1 was revealed with p34-38 (13/34, 38%) and p28-29 (7/34, 21%), and, generally, for 20 of 34 tumor samples(59%, p<0,05). The differences in antigen expression in renal cell carcinomas are statistically significant, as compared with adjacent (presumably normal) tissues. The p34-38 expression was not revealed in serum pellets obtained from 4 cancer patients by ultracentrifugation. A variety of multiple proteins was revealed in one оf seventeen tissue samples surrounding the tumor (normal breast tissue). Interestingly, a difference was found between renal cell carcinoma (p28-29 expression) and adjacent normal tissues (p21-24) that was revealed by means of MKA 2H6. The p21-24 and p28-29 may represent a product of p34-38 posttranslational modifications. It was supposed that a variety of proteins revealed with MKA 1, 2H6 are interrelated, due to identity of some sites. Detection of p34-38, p28-29 with MKA 1 may be potentially used as a supplementary immunological approach, in order to differentiate renal cell carcinoma tissues of epithelial origin from the adjacent tissues. Further studies are needed to elucidate an opportunity of MKA 2H6 usage, aiming for detection of p28-29 in renal cell carcinoma samples, like as p21-24 for specimens of adjacent normal tissues. (Med. Immunol., 2008, vol. 10, N 2-3, pp 209-214).T. S. BobrovaYu. V. ChuevSPb RAACIarticlerenal cell carcinomasmonoclonal antibodiesimmunoblottingImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 2-3, Pp 209-214 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	renal cell carcinomas monoclonal antibodies immunoblotting Immunologic diseases. Allergy RC581-607
spellingShingle	renal cell carcinomas monoclonal antibodies immunoblotting Immunologic diseases. Allergy RC581-607 T. S. Bobrova Yu. V. Chuev EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA
description	Abstract. Samples of renal cell carcinoma (RCC) and adjacent normal tissues were studied by means of immunoblotting, using monoclonal antibodies [(MKA 1, 1F3-2D4, IgG1 class), MKA 2H6, IgG1 class] against membrane proteins of HEp-2 cells (larynx cancer). Strong reactivity of MKA 1 was revealed with p34-38 (13/34, 38%) and p28-29 (7/34, 21%), and, generally, for 20 of 34 tumor samples(59%, p<0,05). The differences in antigen expression in renal cell carcinomas are statistically significant, as compared with adjacent (presumably normal) tissues. The p34-38 expression was not revealed in serum pellets obtained from 4 cancer patients by ultracentrifugation. A variety of multiple proteins was revealed in one оf seventeen tissue samples surrounding the tumor (normal breast tissue). Interestingly, a difference was found between renal cell carcinoma (p28-29 expression) and adjacent normal tissues (p21-24) that was revealed by means of MKA 2H6. The p21-24 and p28-29 may represent a product of p34-38 posttranslational modifications. It was supposed that a variety of proteins revealed with MKA 1, 2H6 are interrelated, due to identity of some sites. Detection of p34-38, p28-29 with MKA 1 may be potentially used as a supplementary immunological approach, in order to differentiate renal cell carcinoma tissues of epithelial origin from the adjacent tissues. Further studies are needed to elucidate an opportunity of MKA 2H6 usage, aiming for detection of p28-29 in renal cell carcinoma samples, like as p21-24 for specimens of adjacent normal tissues. (Med. Immunol., 2008, vol. 10, N 2-3, pp 209-214).
format	article
author	T. S. Bobrova Yu. V. Chuev
author_facet	T. S. Bobrova Yu. V. Chuev
author_sort	T. S. Bobrova
title	EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA
title_short	EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA
title_full	EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA
title_fullStr	EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA
title_full_unstemmed	EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA
title_sort	expression of cancer-associated proteins in tissues and blood serum from patients with renal cell carcinoma
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/c6ea110974204b81b80890024ff4b26d
work_keys_str_mv	AT tsbobrova expressionofcancerassociatedproteinsintissuesandbloodserumfrompatientswithrenalcellcarcinoma AT yuvchuev expressionofcancerassociatedproteinsintissuesandbloodserumfrompatientswithrenalcellcarcinoma
_version_	1718422567478362112

EXPRESSION OF CANCER-ASSOCIATED PROTEINS IN TISSUES AND BLOOD SERUM FROM PATIENTS WITH RENAL CELL CARCINOMA

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