Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Anthocyanins contribute to most colors of plants and play protective roles in response to abiotic stresses. Brassica napus is widely cultivated worldwide as both an oilseed and a vegetable. However, only several high anthocyanin-containing cultivars have been reported, and the mechanisms of anthocya...

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Autores principales:	Dan He, Dawei Zhang, Ting Li, Lili Liu, Dinggang Zhou, Lei Kang, Jinfeng Wu, Zhongsong Liu, Mingli Yan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	anthocyanin biosynthetic genes B. napus comparative genomic analysis transcriptome dynamic accumulation Genetics QH426-470
Acceso en línea:	https://doaj.org/article/c6ee0d9a722a41b68a264adcbd5b4700
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spelling	oai:doaj.org-article:c6ee0d9a722a41b68a264adcbd5b47002021-11-18T06:02:11ZWhole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus1664-802110.3389/fgene.2021.764835https://doaj.org/article/c6ee0d9a722a41b68a264adcbd5b47002021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.764835/fullhttps://doaj.org/toc/1664-8021Anthocyanins contribute to most colors of plants and play protective roles in response to abiotic stresses. Brassica napus is widely cultivated worldwide as both an oilseed and a vegetable. However, only several high anthocyanin-containing cultivars have been reported, and the mechanisms of anthocyanin accumulation have not been well-elucidated in B. napus. Here, the phenotype, comparative whole-genome identification, and gene expression analysis were performed to investigate the dynamic change of the anthocyanin content and the gene expression patterns of anthocyanin biosynthetic genes (ABGs) in B. napus. A total of 152 ABGs were identified in the B. napus reference genome. To screen out the critical genes involved in anthocyanin biosynthesis and accumulation, the RNA-seq of young leaves of two B. napus lines with purple leaves (PL) or green leaves (GL), and their F1 progeny at 41, 91, and 101 days were performed to identify the differentially expressed genes. The comparative expression analysis of these ABGs indicated that the upregulation of TT8 together with its target genes (such as DFR, ANS, UFGT, and TT19) might promote the anthocyanin accumulation in PL at the early developmental stage (41–91 days). While the downregulation of those ABGs and anthocyanin degradation at the late developmental stage (91–101 days) might result in the decrease in anthocyanin accumulation. Our results would enhance the understanding of the regulatory network of anthocyanin dynamic accumulation in B. napus.Dan HeDan HeDawei ZhangDawei ZhangTing LiTing LiLili LiuLili LiuDinggang ZhouDinggang ZhouLei KangJinfeng WuJinfeng WuZhongsong LiuMingli YanMingli YanFrontiers Media S.A.articleanthocyanin biosynthetic genesB. napuscomparative genomic analysistranscriptomedynamic accumulationGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	anthocyanin biosynthetic genes B. napus comparative genomic analysis transcriptome dynamic accumulation Genetics QH426-470
spellingShingle	anthocyanin biosynthetic genes B. napus comparative genomic analysis transcriptome dynamic accumulation Genetics QH426-470 Dan He Dan He Dawei Zhang Dawei Zhang Ting Li Ting Li Lili Liu Lili Liu Dinggang Zhou Dinggang Zhou Lei Kang Jinfeng Wu Jinfeng Wu Zhongsong Liu Mingli Yan Mingli Yan Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus
description	Anthocyanins contribute to most colors of plants and play protective roles in response to abiotic stresses. Brassica napus is widely cultivated worldwide as both an oilseed and a vegetable. However, only several high anthocyanin-containing cultivars have been reported, and the mechanisms of anthocyanin accumulation have not been well-elucidated in B. napus. Here, the phenotype, comparative whole-genome identification, and gene expression analysis were performed to investigate the dynamic change of the anthocyanin content and the gene expression patterns of anthocyanin biosynthetic genes (ABGs) in B. napus. A total of 152 ABGs were identified in the B. napus reference genome. To screen out the critical genes involved in anthocyanin biosynthesis and accumulation, the RNA-seq of young leaves of two B. napus lines with purple leaves (PL) or green leaves (GL), and their F1 progeny at 41, 91, and 101 days were performed to identify the differentially expressed genes. The comparative expression analysis of these ABGs indicated that the upregulation of TT8 together with its target genes (such as DFR, ANS, UFGT, and TT19) might promote the anthocyanin accumulation in PL at the early developmental stage (41–91 days). While the downregulation of those ABGs and anthocyanin degradation at the late developmental stage (91–101 days) might result in the decrease in anthocyanin accumulation. Our results would enhance the understanding of the regulatory network of anthocyanin dynamic accumulation in B. napus.
format	article
author	Dan He Dan He Dawei Zhang Dawei Zhang Ting Li Ting Li Lili Liu Lili Liu Dinggang Zhou Dinggang Zhou Lei Kang Jinfeng Wu Jinfeng Wu Zhongsong Liu Mingli Yan Mingli Yan
author_facet	Dan He Dan He Dawei Zhang Dawei Zhang Ting Li Ting Li Lili Liu Lili Liu Dinggang Zhou Dinggang Zhou Lei Kang Jinfeng Wu Jinfeng Wu Zhongsong Liu Mingli Yan Mingli Yan
author_sort	Dan He
title	Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus
title_short	Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus
title_full	Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus
title_fullStr	Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus
title_full_unstemmed	Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus
title_sort	whole-genome identification and comparative expression analysis of anthocyanin biosynthetic genes in brassica napus
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c6ee0d9a722a41b68a264adcbd5b4700
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Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

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