QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)

Abstract Background Ascochyta blight caused by Ascochyta fabae Speg. and broomrape (Orobanche crenata) are among the economically most significant pathogens of faba bean. Several QTLs conferring resistance against the two pathogens have been identified and validated in different genetic backgrounds....

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Autores principales:	Natalia Gutierrez, Ana M. Torres
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Faba bean Ascochyta Orobanche Resistance Map saturation Medicago Botany QK1-989
Acceso en línea:	https://doaj.org/article/c6f4cf0f69a64df0ae83de53a314bff4
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spelling	oai:doaj.org-article:c6f4cf0f69a64df0ae83de53a314bff42021-11-28T12:07:28ZQTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)10.1186/s12870-021-03335-51471-2229https://doaj.org/article/c6f4cf0f69a64df0ae83de53a314bff42021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03335-5https://doaj.org/toc/1471-2229Abstract Background Ascochyta blight caused by Ascochyta fabae Speg. and broomrape (Orobanche crenata) are among the economically most significant pathogens of faba bean. Several QTLs conferring resistance against the two pathogens have been identified and validated in different genetic backgrounds. The aim of this study was to saturate the most stable QTLs for ascochyta and broomrape resistance in two Recombinant Inbred Line (RIL) populations, 29H x Vf136 and Vf6 x Vf136, to identify candidate genes conferring resistance against these two pathogens. Results We exploited the synteny between faba bean and the model species Medicago truncatula by selecting a set of 219 genes encoding putative WRKY transcription factors and defense related proteins falling within the target QTL intervals, for genotyping and marker saturation in the two RIL populations. Seventy and 50 of the candidate genes could be mapped in 29H x Vf136 and Vf6 x Vf136, respectively. Besides the strong reduction of the QTL intervals, the mapping process allowed replacing previous dominant and pedigree-specific RAPD flanking markers with robust and transferrable SNP markers, revealing promising candidates for resistance against the two pathogens. Conclusions Although further efforts in association mapping and expression studies will be required to corroborate the candidate genes for resistance, the fine-mapping approach proposed here increases the genetic resolution of relevant QTL regions and paves the way for an efficient deployment of useful alleles for faba bean ascochyta and broomrape resistance through marker-assisted breeding.Natalia GutierrezAna M. TorresBMCarticleFaba beanAscochytaOrobancheResistanceMap saturationMedicagoBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Faba bean Ascochyta Orobanche Resistance Map saturation Medicago Botany QK1-989
spellingShingle	Faba bean Ascochyta Orobanche Resistance Map saturation Medicago Botany QK1-989 Natalia Gutierrez Ana M. Torres QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
description	Abstract Background Ascochyta blight caused by Ascochyta fabae Speg. and broomrape (Orobanche crenata) are among the economically most significant pathogens of faba bean. Several QTLs conferring resistance against the two pathogens have been identified and validated in different genetic backgrounds. The aim of this study was to saturate the most stable QTLs for ascochyta and broomrape resistance in two Recombinant Inbred Line (RIL) populations, 29H x Vf136 and Vf6 x Vf136, to identify candidate genes conferring resistance against these two pathogens. Results We exploited the synteny between faba bean and the model species Medicago truncatula by selecting a set of 219 genes encoding putative WRKY transcription factors and defense related proteins falling within the target QTL intervals, for genotyping and marker saturation in the two RIL populations. Seventy and 50 of the candidate genes could be mapped in 29H x Vf136 and Vf6 x Vf136, respectively. Besides the strong reduction of the QTL intervals, the mapping process allowed replacing previous dominant and pedigree-specific RAPD flanking markers with robust and transferrable SNP markers, revealing promising candidates for resistance against the two pathogens. Conclusions Although further efforts in association mapping and expression studies will be required to corroborate the candidate genes for resistance, the fine-mapping approach proposed here increases the genetic resolution of relevant QTL regions and paves the way for an efficient deployment of useful alleles for faba bean ascochyta and broomrape resistance through marker-assisted breeding.
format	article
author	Natalia Gutierrez Ana M. Torres
author_facet	Natalia Gutierrez Ana M. Torres
author_sort	Natalia Gutierrez
title	QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
title_short	QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
title_full	QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
title_fullStr	QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
title_full_unstemmed	QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
title_sort	qtl dissection and mining of candidate genes for ascochyta fabae and orobanche crenata resistance in faba bean (vicia faba l.)
publisher	BMC
publishDate	2021
url	https://doaj.org/article/c6f4cf0f69a64df0ae83de53a314bff4
work_keys_str_mv	AT nataliagutierrez qtldissectionandminingofcandidategenesforascochytafabaeandorobanchecrenataresistanceinfababeanviciafabal AT anamtorres qtldissectionandminingofcandidategenesforascochytafabaeandorobanchecrenataresistanceinfababeanviciafabal
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QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)

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