The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

Abstract Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can provide valuable information related...

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Autores principales:	Yousef Risha, Zoran Minic, Shahrokh M. Ghobadloo, Maxim V. Berezovski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c6f66b9b1b3f4187a8538d48334d1408
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spelling	oai:doaj.org-article:c6f66b9b1b3f4187a8538d48334d14082021-12-02T18:50:59ZThe proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers10.1038/s41598-020-70393-42045-2322https://doaj.org/article/c6f66b9b1b3f4187a8538d48334d14082020-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-70393-4https://doaj.org/toc/2045-2322Abstract Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can provide valuable information related to breast cancer progression and metastasis. Moreover, such a comparison can be explored to find potentially new protein biomarkers for early disease detection. In this study, exosomal proteomes of MDA-MB-231, a metastatic breast cancer cell line, and MCF-10A, a non-cancerous epithelial breast cell line, were identified by nano-liquid chromatography coupled to tandem mass spectrometry. We also tested three exosomes isolation methods (ExoQuick, Ultracentrifugation (UC), and Ultrafiltration–Ultracentrifugation) and detergents (n-dodecyl β-d-maltoside, Triton X-100, and Digitonin) for solubilization of exosomal proteins and enhanced detection by mass spectrometry. A total of 1,107 exosomal proteins were identified in both cell lines, 726 of which were unique to the MDA-MB-231 breast cancer cell line. Among them, 87 proteins were predicted to be relevant to breast cancer and 16 proteins to cancer metastasis. Three exosomal membrane/surface proteins, glucose transporter 1 (GLUT-1), glypican 1 (GPC-1), and disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), were identified as potential breast cancer biomarkers and validated with Western blotting and high-resolution flow cytometry. We demonstrated that exosomes are a rich source of breast cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis.Yousef RishaZoran MinicShahrokh M. GhobadlooMaxim V. BerezovskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yousef Risha Zoran Minic Shahrokh M. Ghobadloo Maxim V. Berezovski The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
description	Abstract Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can provide valuable information related to breast cancer progression and metastasis. Moreover, such a comparison can be explored to find potentially new protein biomarkers for early disease detection. In this study, exosomal proteomes of MDA-MB-231, a metastatic breast cancer cell line, and MCF-10A, a non-cancerous epithelial breast cell line, were identified by nano-liquid chromatography coupled to tandem mass spectrometry. We also tested three exosomes isolation methods (ExoQuick, Ultracentrifugation (UC), and Ultrafiltration–Ultracentrifugation) and detergents (n-dodecyl β-d-maltoside, Triton X-100, and Digitonin) for solubilization of exosomal proteins and enhanced detection by mass spectrometry. A total of 1,107 exosomal proteins were identified in both cell lines, 726 of which were unique to the MDA-MB-231 breast cancer cell line. Among them, 87 proteins were predicted to be relevant to breast cancer and 16 proteins to cancer metastasis. Three exosomal membrane/surface proteins, glucose transporter 1 (GLUT-1), glypican 1 (GPC-1), and disintegrin and metalloproteinase domain-containing protein 10 (ADAM10), were identified as potential breast cancer biomarkers and validated with Western blotting and high-resolution flow cytometry. We demonstrated that exosomes are a rich source of breast cancer-related proteins and surface biomarkers that may be used for disease diagnosis and prognosis.
format	article
author	Yousef Risha Zoran Minic Shahrokh M. Ghobadloo Maxim V. Berezovski
author_facet	Yousef Risha Zoran Minic Shahrokh M. Ghobadloo Maxim V. Berezovski
author_sort	Yousef Risha
title	The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
title_short	The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
title_full	The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
title_fullStr	The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
title_full_unstemmed	The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
title_sort	proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/c6f66b9b1b3f4187a8538d48334d1408
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The proteomic analysis of breast cell line exosomes reveals disease patterns and potential biomarkers

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