Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany

The contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rar...

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Autores principales:	Hossein Masigol, Jason Nicholas Woodhouse, Pieter van West, Reza Mostowfizadeh-Ghalamfarsa, Keilor Rojas-Jimenez, Tobias Goldhammer, Seyed Akbar Khodaparast, Hans-Peter Grossart
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	freshwater ecosystems aquatic carbon cycling plant litter degradation humic substances production <i>Saprolegnia</i> <i>Achlya</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/c6f8031a5af84e48bd79d8fa7e43dbfc
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spelling	oai:doaj.org-article:c6f8031a5af84e48bd79d8fa7e43dbfc2021-11-25T18:06:16ZPhylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany10.3390/jof71109682309-608Xhttps://doaj.org/article/c6f8031a5af84e48bd79d8fa7e43dbfc2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/968https://doaj.org/toc/2309-608XThe contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rarely studied. In this study, we isolated and phylogenetically placed 51 fungal and 62 Saprolegniales strains from 12 German lakes. We studied the cellulo-, lignino-, and chitinolytic activity of the strains using plate assays. Furthermore, we determined the capacity of 10 selected strains to utilize 95 different labile compounds, using Biolog FF MicroPlates™. Finally, the ability of three selected strains to utilize maltose and degrade/produce humic substances was measured. <i>Cladosporium</i> and <i>Penicillium</i> were amongst the most prevalent fungal strains, while <i>Saprolegnia</i>, <i>Achlya</i>, and <i>Leptolegnia</i> were the most frequent Saprolegniales strains. Although the isolated strains assigned to genera were phylogenetically similar, their enzymatic activity and physiological profiling were quite diverse. Our results indicate that Saprolegniales, in contrast to fungi, lack ligninolytic activity and are not involved in the production/transformation of humic substances. We hypothesize that Saprolegniales and fungi might have complementary roles in interacting with dissolved organic matter, which has ecological implications for carbon cycling in freshwater ecosystems.Hossein MasigolJason Nicholas WoodhousePieter van WestReza Mostowfizadeh-GhalamfarsaKeilor Rojas-JimenezTobias GoldhammerSeyed Akbar KhodaparastHans-Peter GrossartMDPI AGarticlefreshwater ecosystemsaquatic carbon cyclingplant litter degradationhumic substances production<i>Saprolegnia</i><i>Achlya</i>Biology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 968, p 968 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	freshwater ecosystems aquatic carbon cycling plant litter degradation humic substances production <i>Saprolegnia</i> <i>Achlya</i> Biology (General) QH301-705.5
spellingShingle	freshwater ecosystems aquatic carbon cycling plant litter degradation humic substances production <i>Saprolegnia</i> <i>Achlya</i> Biology (General) QH301-705.5 Hossein Masigol Jason Nicholas Woodhouse Pieter van West Reza Mostowfizadeh-Ghalamfarsa Keilor Rojas-Jimenez Tobias Goldhammer Seyed Akbar Khodaparast Hans-Peter Grossart Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
description	The contribution of fungi to the degradation of plant litter and transformation of dissolved organic matter (humic substances, in particular) in freshwater ecosystems has received increasing attention recently. However, the role of Saprolegniales as one of the most common eukaryotic organisms is rarely studied. In this study, we isolated and phylogenetically placed 51 fungal and 62 Saprolegniales strains from 12 German lakes. We studied the cellulo-, lignino-, and chitinolytic activity of the strains using plate assays. Furthermore, we determined the capacity of 10 selected strains to utilize 95 different labile compounds, using Biolog FF MicroPlates™. Finally, the ability of three selected strains to utilize maltose and degrade/produce humic substances was measured. <i>Cladosporium</i> and <i>Penicillium</i> were amongst the most prevalent fungal strains, while <i>Saprolegnia</i>, <i>Achlya</i>, and <i>Leptolegnia</i> were the most frequent Saprolegniales strains. Although the isolated strains assigned to genera were phylogenetically similar, their enzymatic activity and physiological profiling were quite diverse. Our results indicate that Saprolegniales, in contrast to fungi, lack ligninolytic activity and are not involved in the production/transformation of humic substances. We hypothesize that Saprolegniales and fungi might have complementary roles in interacting with dissolved organic matter, which has ecological implications for carbon cycling in freshwater ecosystems.
format	article
author	Hossein Masigol Jason Nicholas Woodhouse Pieter van West Reza Mostowfizadeh-Ghalamfarsa Keilor Rojas-Jimenez Tobias Goldhammer Seyed Akbar Khodaparast Hans-Peter Grossart
author_facet	Hossein Masigol Jason Nicholas Woodhouse Pieter van West Reza Mostowfizadeh-Ghalamfarsa Keilor Rojas-Jimenez Tobias Goldhammer Seyed Akbar Khodaparast Hans-Peter Grossart
author_sort	Hossein Masigol
title	Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
title_short	Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
title_full	Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
title_fullStr	Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
title_full_unstemmed	Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany
title_sort	phylogenetic and functional diversity of saprolegniales and fungi isolated from temperate lakes in northeast germany
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/c6f8031a5af84e48bd79d8fa7e43dbfc
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Phylogenetic and Functional Diversity of Saprolegniales and Fungi Isolated from Temperate Lakes in Northeast Germany

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