Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line

A biosurfactant producing bacterium was identified as Pseudomonas aeruginosa DNM50 based on molecular characterization (NCBI accession no. MK351591). Structural characterization using MALDI-TOF revealed the presence of 12 different congeners of rhamnolipid such as Rha-C8-C8:1, Rha-C10-C8:1, Rha-C10-...

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Autores principales:	Neelam Mishra, Kavita Rana, Siva Deepthi Seelam, Rakesh Kumar, Vijyendra Pandey, Bharathi P. Salimath, Dayanand Agsar
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	MDA-MB-231 cell lines rhamnolipid MTT trypan blue p38MAPK TNBC Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/c6f867e64c444ad699994ee646e3e8d5
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spelling	oai:doaj.org-article:c6f867e64c444ad699994ee646e3e8d52021-12-01T18:18:03ZCharacterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line2296-418510.3389/fbioe.2021.761266https://doaj.org/article/c6f867e64c444ad699994ee646e3e8d52021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.761266/fullhttps://doaj.org/toc/2296-4185A biosurfactant producing bacterium was identified as Pseudomonas aeruginosa DNM50 based on molecular characterization (NCBI accession no. MK351591). Structural characterization using MALDI-TOF revealed the presence of 12 different congeners of rhamnolipid such as Rha-C8-C8:1, Rha-C10-C8:1, Rha-C10-C10, Rha-C10-C12:1, Rha-C16:1, Rha-C16, Rha-C17:1, Rha-Rha-C10:1-C10:1, Rha-Rha-C10-C12, Rha-Rha-C10-C8, Rha-Rha-C10-C8:1, and Rha-Rha-C8-C8. The radical scavenging activity of rhamnolipid (DNM50RL) was determined by 2, 3-diphenyl-1-picrylhydrazyl (DPPH) assay which showed an IC50 value of 101.8 μg/ ml. The cytotoxic activity was investigated against MDA-MB-231 breast cancer cell line by MTT (4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay which showed a very low IC50 of 0.05 μg/ ml at 72 h of treatment. Further, its activity was confirmed by resazurin and trypan blue assay with IC50 values of 0.01 μg/ml and 0.64 μg/ ml at 72 h of treatment, respectively. Thus, the DNM50RL would play a vital role in the treatment of breast cancer targeting inhibition of p38MAPK.Neelam MishraKavita RanaSiva Deepthi SeelamRakesh KumarVijyendra PandeyBharathi P. SalimathBharathi P. SalimathDayanand AgsarFrontiers Media S.A.articleMDA-MB-231 cell linesrhamnolipidMTTtrypan bluep38MAPKTNBCBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	MDA-MB-231 cell lines rhamnolipid MTT trypan blue p38MAPK TNBC Biotechnology TP248.13-248.65
spellingShingle	MDA-MB-231 cell lines rhamnolipid MTT trypan blue p38MAPK TNBC Biotechnology TP248.13-248.65 Neelam Mishra Kavita Rana Siva Deepthi Seelam Rakesh Kumar Vijyendra Pandey Bharathi P. Salimath Bharathi P. Salimath Dayanand Agsar Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line
description	A biosurfactant producing bacterium was identified as Pseudomonas aeruginosa DNM50 based on molecular characterization (NCBI accession no. MK351591). Structural characterization using MALDI-TOF revealed the presence of 12 different congeners of rhamnolipid such as Rha-C8-C8:1, Rha-C10-C8:1, Rha-C10-C10, Rha-C10-C12:1, Rha-C16:1, Rha-C16, Rha-C17:1, Rha-Rha-C10:1-C10:1, Rha-Rha-C10-C12, Rha-Rha-C10-C8, Rha-Rha-C10-C8:1, and Rha-Rha-C8-C8. The radical scavenging activity of rhamnolipid (DNM50RL) was determined by 2, 3-diphenyl-1-picrylhydrazyl (DPPH) assay which showed an IC50 value of 101.8 μg/ ml. The cytotoxic activity was investigated against MDA-MB-231 breast cancer cell line by MTT (4,5-dimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay which showed a very low IC50 of 0.05 μg/ ml at 72 h of treatment. Further, its activity was confirmed by resazurin and trypan blue assay with IC50 values of 0.01 μg/ml and 0.64 μg/ ml at 72 h of treatment, respectively. Thus, the DNM50RL would play a vital role in the treatment of breast cancer targeting inhibition of p38MAPK.
format	article
author	Neelam Mishra Kavita Rana Siva Deepthi Seelam Rakesh Kumar Vijyendra Pandey Bharathi P. Salimath Bharathi P. Salimath Dayanand Agsar
author_facet	Neelam Mishra Kavita Rana Siva Deepthi Seelam Rakesh Kumar Vijyendra Pandey Bharathi P. Salimath Bharathi P. Salimath Dayanand Agsar
author_sort	Neelam Mishra
title	Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line
title_short	Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line
title_full	Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line
title_fullStr	Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line
title_full_unstemmed	Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line
title_sort	characterization and cytotoxicity of pseudomonas mediated rhamnolipids against breast cancer mda-mb-231 cell line
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c6f867e64c444ad699994ee646e3e8d5
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Characterization and Cytotoxicity of Pseudomonas Mediated Rhamnolipids Against Breast Cancer MDA-MB-231 Cell Line

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