The Tnt1 retrotransposon escapes silencing in tobacco, its natural host.
Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting t...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2012
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/c6f90c61b6804605a506cd70bf73f711 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:c6f90c61b6804605a506cd70bf73f711 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:c6f90c61b6804605a506cd70bf73f7112021-11-18T07:23:41ZThe Tnt1 retrotransposon escapes silencing in tobacco, its natural host.1932-620310.1371/journal.pone.0033816https://doaj.org/article/c6f90c61b6804605a506cd70bf73f7112012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22479451/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting that, as shown for plant viruses, retrotransposons can escape silencing. However no evidence of retrotransposon silencing escape has been reported. Here we analyze the silencing control of the tobacco Tnt1 retrotransposon and report that even though constructs driven by the Tnt1 promoter become silenced when stably integrated in tobacco, the endogenous Tnt1 elements remain active. Silencing of Tnt1-containing transgenes correlates with high DNA methylation and the inability to incorporate H2A.Z into their promoters, whereas the endogenous Tnt1 elements remain partially methylated at asymmetrical positions and incorporate H2A.Z upon induction. Our results show that the promoter of Tnt1 is a target of silencing in tobacco, but also that endogenous Tnt1 elements can escape this control and be expressed in their natural host.Inmaculada Hernández-PinzónMarta CifuentesElizabeth HénaffNéstor SantiagoM Lluïsa EspinásJosep M CasacubertaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33816 (2012) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Inmaculada Hernández-Pinzón Marta Cifuentes Elizabeth Hénaff Néstor Santiago M Lluïsa Espinás Josep M Casacuberta The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| description |
Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting that, as shown for plant viruses, retrotransposons can escape silencing. However no evidence of retrotransposon silencing escape has been reported. Here we analyze the silencing control of the tobacco Tnt1 retrotransposon and report that even though constructs driven by the Tnt1 promoter become silenced when stably integrated in tobacco, the endogenous Tnt1 elements remain active. Silencing of Tnt1-containing transgenes correlates with high DNA methylation and the inability to incorporate H2A.Z into their promoters, whereas the endogenous Tnt1 elements remain partially methylated at asymmetrical positions and incorporate H2A.Z upon induction. Our results show that the promoter of Tnt1 is a target of silencing in tobacco, but also that endogenous Tnt1 elements can escape this control and be expressed in their natural host. |
| format |
article |
| author |
Inmaculada Hernández-Pinzón Marta Cifuentes Elizabeth Hénaff Néstor Santiago M Lluïsa Espinás Josep M Casacuberta |
| author_facet |
Inmaculada Hernández-Pinzón Marta Cifuentes Elizabeth Hénaff Néstor Santiago M Lluïsa Espinás Josep M Casacuberta |
| author_sort |
Inmaculada Hernández-Pinzón |
| title |
The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| title_short |
The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| title_full |
The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| title_fullStr |
The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| title_full_unstemmed |
The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| title_sort |
tnt1 retrotransposon escapes silencing in tobacco, its natural host. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/c6f90c61b6804605a506cd70bf73f711 |
| work_keys_str_mv |
AT inmaculadahernandezpinzon thetnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT martacifuentes thetnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT elizabethhenaff thetnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT nestorsantiago thetnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT mlluisaespinas thetnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT josepmcasacuberta thetnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT inmaculadahernandezpinzon tnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT martacifuentes tnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT elizabethhenaff tnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT nestorsantiago tnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT mlluisaespinas tnt1retrotransposonescapessilencingintobaccoitsnaturalhost AT josepmcasacuberta tnt1retrotransposonescapessilencingintobaccoitsnaturalhost |
| _version_ |
1718423562389291008 |