Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.

Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like virus from plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists of 60 identical coat pro...

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Autores principales: Pascale Schellenberger, Claude Sauter, Bernard Lorber, Patrick Bron, Stefano Trapani, Marc Bergdoll, Aurélie Marmonier, Corinne Schmitt-Keichinger, Olivier Lemaire, Gérard Demangeat, Christophe Ritzenthaler
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:c6fdbe85834e4a76a31f75213ee57b4b2021-11-18T06:03:22ZStructural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.1553-73661553-737410.1371/journal.ppat.1002034https://doaj.org/article/c6fdbe85834e4a76a31f75213ee57b4b2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21625570/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like virus from plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists of 60 identical coat protein subunits (CP), carries the determinants of this specificity. Here, we provide novel insight into GFLV transmission by nematodes through a comparative structural and functional analysis of two GFLV variants. We isolated a mutant GFLV strain (GFLV-TD) poorly transmissible by nematodes, and showed that the transmission defect is due to a glycine to aspartate mutation at position 297 (Gly297Asp) in the CP. We next determined the crystal structures of the wild-type GFLV strain F13 at 3.0 Å and of GFLV-TD at 2.7 Å resolution. The Gly297Asp mutation mapped to an exposed loop at the outer surface of the capsid and did not affect the conformation of the assembled capsid, nor of individual CP molecules. The loop is part of a positively charged pocket that includes a previously identified determinant of transmission. We propose that this pocket is a ligand-binding site with essential function in GFLV transmission by X. index. Our data suggest that perturbation of the electrostatic landscape of this pocket affects the interaction of the virion with specific receptors of the nematode's feeding apparatus, and thereby severely diminishes its transmission efficiency. These data provide a first structural insight into the interactions between a plant virus and a nematode vector.Pascale SchellenbergerClaude SauterBernard LorberPatrick BronStefano TrapaniMarc BergdollAurélie MarmonierCorinne Schmitt-KeichingerOlivier LemaireGérard DemangeatChristophe RitzenthalerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 5, p e1002034 (2011)
institution DOAJ
collection DOAJ
language EN
topic Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
spellingShingle Immunologic diseases. Allergy
RC581-607
Biology (General)
QH301-705.5
Pascale Schellenberger
Claude Sauter
Bernard Lorber
Patrick Bron
Stefano Trapani
Marc Bergdoll
Aurélie Marmonier
Corinne Schmitt-Keichinger
Olivier Lemaire
Gérard Demangeat
Christophe Ritzenthaler
Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
description Many animal and plant viruses rely on vectors for their transmission from host to host. Grapevine fanleaf virus (GFLV), a picorna-like virus from plants, is transmitted specifically by the ectoparasitic nematode Xiphinema index. The icosahedral capsid of GFLV, which consists of 60 identical coat protein subunits (CP), carries the determinants of this specificity. Here, we provide novel insight into GFLV transmission by nematodes through a comparative structural and functional analysis of two GFLV variants. We isolated a mutant GFLV strain (GFLV-TD) poorly transmissible by nematodes, and showed that the transmission defect is due to a glycine to aspartate mutation at position 297 (Gly297Asp) in the CP. We next determined the crystal structures of the wild-type GFLV strain F13 at 3.0 Å and of GFLV-TD at 2.7 Å resolution. The Gly297Asp mutation mapped to an exposed loop at the outer surface of the capsid and did not affect the conformation of the assembled capsid, nor of individual CP molecules. The loop is part of a positively charged pocket that includes a previously identified determinant of transmission. We propose that this pocket is a ligand-binding site with essential function in GFLV transmission by X. index. Our data suggest that perturbation of the electrostatic landscape of this pocket affects the interaction of the virion with specific receptors of the nematode's feeding apparatus, and thereby severely diminishes its transmission efficiency. These data provide a first structural insight into the interactions between a plant virus and a nematode vector.
format article
author Pascale Schellenberger
Claude Sauter
Bernard Lorber
Patrick Bron
Stefano Trapani
Marc Bergdoll
Aurélie Marmonier
Corinne Schmitt-Keichinger
Olivier Lemaire
Gérard Demangeat
Christophe Ritzenthaler
author_facet Pascale Schellenberger
Claude Sauter
Bernard Lorber
Patrick Bron
Stefano Trapani
Marc Bergdoll
Aurélie Marmonier
Corinne Schmitt-Keichinger
Olivier Lemaire
Gérard Demangeat
Christophe Ritzenthaler
author_sort Pascale Schellenberger
title Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
title_short Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
title_full Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
title_fullStr Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
title_full_unstemmed Structural insights into viral determinants of nematode mediated Grapevine fanleaf virus transmission.
title_sort structural insights into viral determinants of nematode mediated grapevine fanleaf virus transmission.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/c6fdbe85834e4a76a31f75213ee57b4b
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