Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements

Methods for a dissolution study by ultra-high performance liquid chromatography/triple quadrupole mass spectrometry (UHPLC-QqQ/MS) analysis of grape polyphenol dietary supplements, namely, grape seed extract (GSE) and resveratrol (RSV) capsules, were developed following the guidance of United States...

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Autores principales:	Weiting Lyu, Thamer Omar, Harna Patel, David Rodriguez, Mario G. Ferruzzi, Giulio M. Pasinetti, James W. Murrough, Fernando J. Muzzio, James E. Simon, Qingli Wu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	grape seed extract resveratrol UHPLC-QQQ/MS polyphenol bioavailability Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/c70953b05b724a3eadd9e97bf1bf49d6
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spelling	oai:doaj.org-article:c70953b05b724a3eadd9e97bf1bf49d62021-12-01T02:36:55ZDissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements2296-861X10.3389/fnut.2021.780260https://doaj.org/article/c70953b05b724a3eadd9e97bf1bf49d62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.780260/fullhttps://doaj.org/toc/2296-861XMethods for a dissolution study by ultra-high performance liquid chromatography/triple quadrupole mass spectrometry (UHPLC-QqQ/MS) analysis of grape polyphenol dietary supplements, namely, grape seed extract (GSE) and resveratrol (RSV) capsules, were developed following the guidance of United States Pharmacopeia (USP) <2040>. Two dissolution media, 0.1 N hydrochloric acid (pH 1.2) and 0.05 M acetate buffer (pH 4.6), were evaluated with dissolution apparatus (USP 1), 100 rpm rotation speed, and 900 ml dissolution medium volume. Dissolution profiling was performed over 120 min. Major phenolic compounds of gallic acid, catechin, epicatechin, and procyanidin B2 were quantitated to obtain the dissolution profile of GSE capsules, and trans-RSV was used for RSV capsules. Results indicated that the released trans-RSV for RSV capsules in both of the dissolution media meets the USP standards, and that for the GSE capsules, all the four marker compounds passed the dissolution test in the HCl medium but did not reach a 75% release within 60 min in the acetate buffer. These promising results suggest that the general USP dissolution protocols are adequate for the successful release of RSV capsules in HCl medium and acetate buffer and GSE capsules (in HCl medium), but may be inadequate for GSE capsules in acetate buffer. These results showed that under a low pH of 1.2 (simulated stomach environment), bioactive compounds were released on time from the GSE capsules and met the USP guidelines; however, under a higher pH of 4.6 (simulated duodenum environment), the same biomarkers failed, suggesting the need to further improve the dissolution of GSE over a wider range of pH environments to enhance bioavailability and efficacy.Weiting LyuWeiting LyuThamer OmarHarna PatelDavid RodriguezMario G. FerruzziGiulio M. PasinettiGiulio M. PasinettiJames W. MurroughJames W. MurroughFernando J. MuzzioJames E. SimonJames E. SimonQingli WuQingli WuFrontiers Media S.A.articlegrape seed extractresveratrolUHPLC-QQQ/MSpolyphenolbioavailabilityNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	grape seed extract resveratrol UHPLC-QQQ/MS polyphenol bioavailability Nutrition. Foods and food supply TX341-641
spellingShingle	grape seed extract resveratrol UHPLC-QQQ/MS polyphenol bioavailability Nutrition. Foods and food supply TX341-641 Weiting Lyu Weiting Lyu Thamer Omar Harna Patel David Rodriguez Mario G. Ferruzzi Giulio M. Pasinetti Giulio M. Pasinetti James W. Murrough James W. Murrough Fernando J. Muzzio James E. Simon James E. Simon Qingli Wu Qingli Wu Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements
description	Methods for a dissolution study by ultra-high performance liquid chromatography/triple quadrupole mass spectrometry (UHPLC-QqQ/MS) analysis of grape polyphenol dietary supplements, namely, grape seed extract (GSE) and resveratrol (RSV) capsules, were developed following the guidance of United States Pharmacopeia (USP) <2040>. Two dissolution media, 0.1 N hydrochloric acid (pH 1.2) and 0.05 M acetate buffer (pH 4.6), were evaluated with dissolution apparatus (USP 1), 100 rpm rotation speed, and 900 ml dissolution medium volume. Dissolution profiling was performed over 120 min. Major phenolic compounds of gallic acid, catechin, epicatechin, and procyanidin B2 were quantitated to obtain the dissolution profile of GSE capsules, and trans-RSV was used for RSV capsules. Results indicated that the released trans-RSV for RSV capsules in both of the dissolution media meets the USP standards, and that for the GSE capsules, all the four marker compounds passed the dissolution test in the HCl medium but did not reach a 75% release within 60 min in the acetate buffer. These promising results suggest that the general USP dissolution protocols are adequate for the successful release of RSV capsules in HCl medium and acetate buffer and GSE capsules (in HCl medium), but may be inadequate for GSE capsules in acetate buffer. These results showed that under a low pH of 1.2 (simulated stomach environment), bioactive compounds were released on time from the GSE capsules and met the USP guidelines; however, under a higher pH of 4.6 (simulated duodenum environment), the same biomarkers failed, suggesting the need to further improve the dissolution of GSE over a wider range of pH environments to enhance bioavailability and efficacy.
format	article
author	Weiting Lyu Weiting Lyu Thamer Omar Harna Patel David Rodriguez Mario G. Ferruzzi Giulio M. Pasinetti Giulio M. Pasinetti James W. Murrough James W. Murrough Fernando J. Muzzio James E. Simon James E. Simon Qingli Wu Qingli Wu
author_facet	Weiting Lyu Weiting Lyu Thamer Omar Harna Patel David Rodriguez Mario G. Ferruzzi Giulio M. Pasinetti Giulio M. Pasinetti James W. Murrough James W. Murrough Fernando J. Muzzio James E. Simon James E. Simon Qingli Wu Qingli Wu
author_sort	Weiting Lyu
title	Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements
title_short	Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements
title_full	Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements
title_fullStr	Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements
title_full_unstemmed	Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements
title_sort	dissolution study on grape polyphenol hard gelatin capsule dietary supplements
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/c70953b05b724a3eadd9e97bf1bf49d6
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Dissolution Study on Grape Polyphenol Hard Gelatin Capsule Dietary Supplements

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