Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats

Background: Visual Evoked Potential (VEP) quantifies electrical signals produced in visual cortex in response to visual stimuli. VEP elicited by light flashes is a useful biomarker to evaluate visual function in preclinical models and it can be recorded in awake or anaesthetised state. Different typ...

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Autores principales:	Valerio Castoldi, Raffaele d’Isa, Silvia Marenna, Giancarlo Comi, Letizia Leocani
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Visual evoked potential Sevoflurane Ketamine-xylazine Repeatability indices Signal-to-noise ratio Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/c70b0d78377e4baca8ada308c4af6986
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spelling	oai:doaj.org-article:c70b0d78377e4baca8ada308c4af69862021-12-02T05:02:50ZNon-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats2405-844010.1016/j.heliyon.2021.e08360https://doaj.org/article/c70b0d78377e4baca8ada308c4af69862021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021024634https://doaj.org/toc/2405-8440Background: Visual Evoked Potential (VEP) quantifies electrical signals produced in visual cortex in response to visual stimuli. VEP elicited by light flashes is a useful biomarker to evaluate visual function in preclinical models and it can be recorded in awake or anaesthetised state. Different types of anaesthesia influence VEP properties, such as latency, which measures the propagation speed along nerve fibers, and amplitude that quantifies the power of electrical signal. Aim: The goal of this work is to compare VEPs elicited in Dark Agouti rats under two types of anaesthesia: volatile sevoflurane or injectable ketamine-xylazine. Methods: VEP latency, amplitude, signal-to-noise ratio and recording duration were measured in Dark Agouti rats randomly assigned to two groups, the first subjected to volatile sevoflurane and the second to injectable ketamine-xylazine. Taking advantage of non-invasive flash-VEP recording through epidermal cup electrodes, three time points of VEP recordings were assessed in two weeks intervals. Results: VEP recorded under ketamine-xylazine showed longer latency and higher amplitude compared with sevoflurane, with analogous repeatability over time. However, sevoflurane tended to suppress electrical signals from visual cortex, resulting in a lower signal-to-noise ratio. Moreover, VEP procedure duration lasted longer in rats anaesthetised with sevoflurane than ketamine-xylazine. Conclusions: In Dark Agouti rats, the use of different anaesthesia can influence VEP components in terms of latency and amplitude. Notably, sevoflurane and ketamine-xylazine revealed satisfying repeatability over time, which is critical to perform reliable follow-up studies. Ketamine-xylazine allowed to obtain more clearly discernible VEP components and less background noise, together with a quicker recording procedure and a consequently improved animal safety and welfare.Valerio CastoldiRaffaele d’IsaSilvia MarennaGiancarlo ComiLetizia LeocaniElsevierarticleVisual evoked potentialSevofluraneKetamine-xylazineRepeatability indicesSignal-to-noise ratioScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08360- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Visual evoked potential Sevoflurane Ketamine-xylazine Repeatability indices Signal-to-noise ratio Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Visual evoked potential Sevoflurane Ketamine-xylazine Repeatability indices Signal-to-noise ratio Science (General) Q1-390 Social sciences (General) H1-99 Valerio Castoldi Raffaele d’Isa Silvia Marenna Giancarlo Comi Letizia Leocani Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
description	Background: Visual Evoked Potential (VEP) quantifies electrical signals produced in visual cortex in response to visual stimuli. VEP elicited by light flashes is a useful biomarker to evaluate visual function in preclinical models and it can be recorded in awake or anaesthetised state. Different types of anaesthesia influence VEP properties, such as latency, which measures the propagation speed along nerve fibers, and amplitude that quantifies the power of electrical signal. Aim: The goal of this work is to compare VEPs elicited in Dark Agouti rats under two types of anaesthesia: volatile sevoflurane or injectable ketamine-xylazine. Methods: VEP latency, amplitude, signal-to-noise ratio and recording duration were measured in Dark Agouti rats randomly assigned to two groups, the first subjected to volatile sevoflurane and the second to injectable ketamine-xylazine. Taking advantage of non-invasive flash-VEP recording through epidermal cup electrodes, three time points of VEP recordings were assessed in two weeks intervals. Results: VEP recorded under ketamine-xylazine showed longer latency and higher amplitude compared with sevoflurane, with analogous repeatability over time. However, sevoflurane tended to suppress electrical signals from visual cortex, resulting in a lower signal-to-noise ratio. Moreover, VEP procedure duration lasted longer in rats anaesthetised with sevoflurane than ketamine-xylazine. Conclusions: In Dark Agouti rats, the use of different anaesthesia can influence VEP components in terms of latency and amplitude. Notably, sevoflurane and ketamine-xylazine revealed satisfying repeatability over time, which is critical to perform reliable follow-up studies. Ketamine-xylazine allowed to obtain more clearly discernible VEP components and less background noise, together with a quicker recording procedure and a consequently improved animal safety and welfare.
format	article
author	Valerio Castoldi Raffaele d’Isa Silvia Marenna Giancarlo Comi Letizia Leocani
author_facet	Valerio Castoldi Raffaele d’Isa Silvia Marenna Giancarlo Comi Letizia Leocani
author_sort	Valerio Castoldi
title	Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
title_short	Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
title_full	Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
title_fullStr	Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
title_full_unstemmed	Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
title_sort	non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/c70b0d78377e4baca8ada308c4af6986
work_keys_str_mv	AT valeriocastoldi noninvasivevisualevokedpotentialsundersevofluraneversusketaminexylazineinrats AT raffaeledisa noninvasivevisualevokedpotentialsundersevofluraneversusketaminexylazineinrats AT silviamarenna noninvasivevisualevokedpotentialsundersevofluraneversusketaminexylazineinrats AT giancarlocomi noninvasivevisualevokedpotentialsundersevofluraneversusketaminexylazineinrats AT letizialeocani noninvasivevisualevokedpotentialsundersevofluraneversusketaminexylazineinrats
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Non-invasive visual evoked potentials under sevoflurane versus ketamine-xylazine in rats

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