Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells

Abstract The conformation and function of a subset of serine and threonine-phosphorylated proteins are regulated by the prolyl isomerase Pin1 through isomerization of phosphorylated Ser/Thr-Pro bonds. Pin1 is intensely expressed in Sertoli cells, but its function in this post mitotic cell remains un...

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Autores principales:	Rabia Islam, Heein Yoon, Bong-soo Kim, Han-sol Bae, Hye-rim Shin, Woo-Jin Kim, Won-joon Yoon, Yun-Sil Lee, Kyung Mi Woo, Jeong-Hwa Baek, Hyun-Mo Ryoo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c70b3e12080e47299670d20625366f94
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spelling	oai:doaj.org-article:c70b3e12080e47299670d20625366f942021-12-02T11:52:39ZBlood-testis barrier integrity depends on Pin1 expression in Sertoli cells10.1038/s41598-017-07229-12045-2322https://doaj.org/article/c70b3e12080e47299670d20625366f942017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07229-1https://doaj.org/toc/2045-2322Abstract The conformation and function of a subset of serine and threonine-phosphorylated proteins are regulated by the prolyl isomerase Pin1 through isomerization of phosphorylated Ser/Thr-Pro bonds. Pin1 is intensely expressed in Sertoli cells, but its function in this post mitotic cell remains unclear. Our aim was to investigate the role of Pin1 in the Sertoli cells. Lack of Pin1 caused disruption of the blood-testis barrier. We next investigated if the activin pathways in the Sertoli cells were affected by lack of Pin1 through immunostaining for Smad3 protein in testis tissue. Indeed, lack of Pin1 caused reduced Smad3 expression in the testis tissue, as well as a reduction in the level of N-Cadherin, a known target of Smad3. Pin1−/− testes express Sertoli cell marker mRNAs in a pattern similar to that seen in Smad3+/− mice, except for an increase in Wt1 expression. The resulting dysregulation of N-Cadherin, connexin 43, and Wt1 targets caused by lack of Pin1 might affect the mesenchymal–epithelial balance in the Sertoli cells and perturb the blood-testis barrier. The effect of Pin1 dosage in Sertoli cells might be useful in the study of toxicant-mediated infertility, gonadal cancer, and for designing male contraceptives.Rabia IslamHeein YoonBong-soo KimHan-sol BaeHye-rim ShinWoo-Jin KimWon-joon YoonYun-Sil LeeKyung Mi WooJeong-Hwa BaekHyun-Mo RyooNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rabia Islam Heein Yoon Bong-soo Kim Han-sol Bae Hye-rim Shin Woo-Jin Kim Won-joon Yoon Yun-Sil Lee Kyung Mi Woo Jeong-Hwa Baek Hyun-Mo Ryoo Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
description	Abstract The conformation and function of a subset of serine and threonine-phosphorylated proteins are regulated by the prolyl isomerase Pin1 through isomerization of phosphorylated Ser/Thr-Pro bonds. Pin1 is intensely expressed in Sertoli cells, but its function in this post mitotic cell remains unclear. Our aim was to investigate the role of Pin1 in the Sertoli cells. Lack of Pin1 caused disruption of the blood-testis barrier. We next investigated if the activin pathways in the Sertoli cells were affected by lack of Pin1 through immunostaining for Smad3 protein in testis tissue. Indeed, lack of Pin1 caused reduced Smad3 expression in the testis tissue, as well as a reduction in the level of N-Cadherin, a known target of Smad3. Pin1−/− testes express Sertoli cell marker mRNAs in a pattern similar to that seen in Smad3+/− mice, except for an increase in Wt1 expression. The resulting dysregulation of N-Cadherin, connexin 43, and Wt1 targets caused by lack of Pin1 might affect the mesenchymal–epithelial balance in the Sertoli cells and perturb the blood-testis barrier. The effect of Pin1 dosage in Sertoli cells might be useful in the study of toxicant-mediated infertility, gonadal cancer, and for designing male contraceptives.
format	article
author	Rabia Islam Heein Yoon Bong-soo Kim Han-sol Bae Hye-rim Shin Woo-Jin Kim Won-joon Yoon Yun-Sil Lee Kyung Mi Woo Jeong-Hwa Baek Hyun-Mo Ryoo
author_facet	Rabia Islam Heein Yoon Bong-soo Kim Han-sol Bae Hye-rim Shin Woo-Jin Kim Won-joon Yoon Yun-Sil Lee Kyung Mi Woo Jeong-Hwa Baek Hyun-Mo Ryoo
author_sort	Rabia Islam
title	Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
title_short	Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
title_full	Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
title_fullStr	Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
title_full_unstemmed	Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells
title_sort	blood-testis barrier integrity depends on pin1 expression in sertoli cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/c70b3e12080e47299670d20625366f94
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Blood-testis barrier integrity depends on Pin1 expression in Sertoli cells

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