Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia

Abstract The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively....

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Autores principales:	Yung-Chih Wang, Tzu-Wen Huang, Ya-Sung Yang, Shu-Chen Kuo, Chung-Ting Chen, Chang-Pan Liu, Yuag-Meng Liu, Te-Li Chen, Feng-Yee Chang, Shih-Hsiung Wu, Chorng-Kuang How, Yi-Tzu Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c70be8b3beaa4f5da3654337f0f6db9d
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spelling	oai:doaj.org-article:c70be8b3beaa4f5da3654337f0f6db9d2021-12-02T15:08:23ZBiofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia10.1038/s41598-018-25661-92045-2322https://doaj.org/article/c70be8b3beaa4f5da3654337f0f6db9d2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25661-9https://doaj.org/toc/2045-2322Abstract The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively. The clinical features and outcomes of patients were investigated. Biofilm formation was determined by a microtitre plate assay. The antimicrobial susceptibilities of biofilm-associated cells were assessed using the minimum biofilm eradication concentration (MBEC) assay. Whole-genome sequencing was conducted to identify biofilm-associated genes and their promoters. Quantitative reverse transcription polymerase chain reaction was performed to confirm the expression difference of biofilm-associated genes. There was no significant difference in the clinical characteristics or the outcomes between patients infected with biofilm-forming and non-biofilm-forming strains. Compared with non-biofilm-forming isolates, biofilm-forming isolates exhibited lower resistance to most antimicrobials tested, including imipenem, meropenem, ceftazidime, ciprofloxacin and gentamicin; however, the MBEC assay confirmed the increased antibiotic resistance of the biofilm-embedded bacteria. Biofilm-associated genes and their promoters were detected in most isolates, including the non-biofilm-forming strains. Biofilm-forming isolates showed higher levels of expression of the biofilm-associated genes than non-biofilm-forming isolates. The biofilm-forming ability of A. baumannii isolates might not be associated with worse outcomes in patients with bacteraemic pneumonia.Yung-Chih WangTzu-Wen HuangYa-Sung YangShu-Chen KuoChung-Ting ChenChang-Pan LiuYuag-Meng LiuTe-Li ChenFeng-Yee ChangShih-Hsiung WuChorng-Kuang HowYi-Tzu LeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yung-Chih Wang Tzu-Wen Huang Ya-Sung Yang Shu-Chen Kuo Chung-Ting Chen Chang-Pan Liu Yuag-Meng Liu Te-Li Chen Feng-Yee Chang Shih-Hsiung Wu Chorng-Kuang How Yi-Tzu Lee Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
description	Abstract The effect of biofilm formation on bacteraemic pneumonia caused by A. baumannii is unknown. We conducted a 4-year multi-center retrospective study to analyze 71 and 202 patients with A. baumannii bacteraemic pneumonia caused by biofilm-forming and non-biofilm-forming isolates, respectively. The clinical features and outcomes of patients were investigated. Biofilm formation was determined by a microtitre plate assay. The antimicrobial susceptibilities of biofilm-associated cells were assessed using the minimum biofilm eradication concentration (MBEC) assay. Whole-genome sequencing was conducted to identify biofilm-associated genes and their promoters. Quantitative reverse transcription polymerase chain reaction was performed to confirm the expression difference of biofilm-associated genes. There was no significant difference in the clinical characteristics or the outcomes between patients infected with biofilm-forming and non-biofilm-forming strains. Compared with non-biofilm-forming isolates, biofilm-forming isolates exhibited lower resistance to most antimicrobials tested, including imipenem, meropenem, ceftazidime, ciprofloxacin and gentamicin; however, the MBEC assay confirmed the increased antibiotic resistance of the biofilm-embedded bacteria. Biofilm-associated genes and their promoters were detected in most isolates, including the non-biofilm-forming strains. Biofilm-forming isolates showed higher levels of expression of the biofilm-associated genes than non-biofilm-forming isolates. The biofilm-forming ability of A. baumannii isolates might not be associated with worse outcomes in patients with bacteraemic pneumonia.
format	article
author	Yung-Chih Wang Tzu-Wen Huang Ya-Sung Yang Shu-Chen Kuo Chung-Ting Chen Chang-Pan Liu Yuag-Meng Liu Te-Li Chen Feng-Yee Chang Shih-Hsiung Wu Chorng-Kuang How Yi-Tzu Lee
author_facet	Yung-Chih Wang Tzu-Wen Huang Ya-Sung Yang Shu-Chen Kuo Chung-Ting Chen Chang-Pan Liu Yuag-Meng Liu Te-Li Chen Feng-Yee Chang Shih-Hsiung Wu Chorng-Kuang How Yi-Tzu Lee
author_sort	Yung-Chih Wang
title	Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
title_short	Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
title_full	Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
title_fullStr	Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
title_full_unstemmed	Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia
title_sort	biofilm formation is not associated with worse outcome in acinetobacter baumannii bacteraemic pneumonia
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/c70be8b3beaa4f5da3654337f0f6db9d
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Biofilm formation is not associated with worse outcome in Acinetobacter baumannii bacteraemic pneumonia

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