Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195

Abstract A synbiotic containing Lactiplantibacillus plantarum [American Type Culture Collection (ATCC) strain identifier 202195] and fructooligosaccharide was reported to reduce the risk of sepsis in young infants in rural India. Here, the whole genome of two isolates of L. plantarum ATCC 202195, wh...

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Autores principales:	Lisa G. Pell, Rachael G. Horne, Stuart Huntley, Hafizur Rahman, Sanchita Kar, Mohammad Shahidul Islam, Kara C. Evans, Samir K. Saha, Aaron Campigotto, Shaun K. Morris, Daniel E. Roth, Philip M. Sherman
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7297a7b959047a1beb8f61f168a7508
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spelling	oai:doaj.org-article:c7297a7b959047a1beb8f61f168a75082021-12-02T18:49:27ZAntimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 20219510.1038/s41598-021-94997-62045-2322https://doaj.org/article/c7297a7b959047a1beb8f61f168a75082021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94997-6https://doaj.org/toc/2045-2322Abstract A synbiotic containing Lactiplantibacillus plantarum [American Type Culture Collection (ATCC) strain identifier 202195] and fructooligosaccharide was reported to reduce the risk of sepsis in young infants in rural India. Here, the whole genome of two isolates of L. plantarum ATCC 202195, which were deposited to the ATCC approximately 20 years apart, were sequenced and analyzed to verify their taxonomic and strain-level identities, identify potential antimicrobial resistant genes and virulence factors, and identify genetic characteristics that may explain the observed clinical effects of L. plantarum ATCC 202195. Minimum inhibitory concentrations for selected antimicrobial agents were determined using broth dilution and gradient strip diffusion techniques. The two L. plantarum ATCC 202195 isolates were genetically identical with only three high-quality single nucleotides polymorphisms identified, and with an average nucleotide identity of 99.99%. In contrast to previously published reports, this study determined that each isolate contained two putative plasmids. No concerning acquired or transferable antimicrobial resistance genes or virulence factors were identified. Both isolates were sensitive to several clinically important antibiotics including penicillin, ampicillin and gentamicin, but resistant to vancomycin. Genes involved in stress response, cellular adhesion, carbohydrate metabolism and vitamin biosynthesis are consistent with features of probiotic organisms.Lisa G. PellRachael G. HorneStuart HuntleyHafizur RahmanSanchita KarMohammad Shahidul IslamKara C. EvansSamir K. SahaAaron CampigottoShaun K. MorrisDaniel E. RothPhilip M. ShermanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa G. Pell Rachael G. Horne Stuart Huntley Hafizur Rahman Sanchita Kar Mohammad Shahidul Islam Kara C. Evans Samir K. Saha Aaron Campigotto Shaun K. Morris Daniel E. Roth Philip M. Sherman Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195
description	Abstract A synbiotic containing Lactiplantibacillus plantarum [American Type Culture Collection (ATCC) strain identifier 202195] and fructooligosaccharide was reported to reduce the risk of sepsis in young infants in rural India. Here, the whole genome of two isolates of L. plantarum ATCC 202195, which were deposited to the ATCC approximately 20 years apart, were sequenced and analyzed to verify their taxonomic and strain-level identities, identify potential antimicrobial resistant genes and virulence factors, and identify genetic characteristics that may explain the observed clinical effects of L. plantarum ATCC 202195. Minimum inhibitory concentrations for selected antimicrobial agents were determined using broth dilution and gradient strip diffusion techniques. The two L. plantarum ATCC 202195 isolates were genetically identical with only three high-quality single nucleotides polymorphisms identified, and with an average nucleotide identity of 99.99%. In contrast to previously published reports, this study determined that each isolate contained two putative plasmids. No concerning acquired or transferable antimicrobial resistance genes or virulence factors were identified. Both isolates were sensitive to several clinically important antibiotics including penicillin, ampicillin and gentamicin, but resistant to vancomycin. Genes involved in stress response, cellular adhesion, carbohydrate metabolism and vitamin biosynthesis are consistent with features of probiotic organisms.
format	article
author	Lisa G. Pell Rachael G. Horne Stuart Huntley Hafizur Rahman Sanchita Kar Mohammad Shahidul Islam Kara C. Evans Samir K. Saha Aaron Campigotto Shaun K. Morris Daniel E. Roth Philip M. Sherman
author_facet	Lisa G. Pell Rachael G. Horne Stuart Huntley Hafizur Rahman Sanchita Kar Mohammad Shahidul Islam Kara C. Evans Samir K. Saha Aaron Campigotto Shaun K. Morris Daniel E. Roth Philip M. Sherman
author_sort	Lisa G. Pell
title	Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195
title_short	Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195
title_full	Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195
title_fullStr	Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195
title_full_unstemmed	Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195
title_sort	antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium lactiplantibacillus plantarum, strain atcc 202195
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c7297a7b959047a1beb8f61f168a7508
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Antimicrobial susceptibilities and comparative whole genome analysis of two isolates of the probiotic bacterium Lactiplantibacillus plantarum, strain ATCC 202195

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