Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.

Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here soug...

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Autores principales:	Anna Llach, Cristina E Molina, Enrique Alvarez-Lacalle, Lluis Tort, Raul Benítez, Leif Hove-Madsen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c737d8d9e9b44a088cc37ae101487794
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spelling	oai:doaj.org-article:c737d8d9e9b44a088cc37ae1014877942021-11-18T06:47:07ZDetection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.1932-620310.1371/journal.pone.0023708https://doaj.org/article/c737d8d9e9b44a088cc37ae1014877942011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21897853/?tool=EBIhttps://doaj.org/toc/1932-6203Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.45±0.03 times the baseline fluorescence and the time to half maximal decay of sparks was 27±3 ms. Spark frequency was 0.88 sparks µm(-1) min(-1) while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F(0)) from 1.77±0.17 to 1.12±0.18 (p = 0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.6±0.3 min(-1) to 8.1±2.0 min(-1), p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.Anna LlachCristina E MolinaEnrique Alvarez-LacalleLluis TortRaul BenítezLeif Hove-MadsenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23708 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Llach Cristina E Molina Enrique Alvarez-Lacalle Lluis Tort Raul Benítez Leif Hove-Madsen Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
description	Calcium release from the sarcoplasmic reticulum (SR) plays a central role in the regulation of cardiac contraction and rhythm in mammals and humans but its role is controversial in teleosts. Since the zebrafish is an emerging model for studies of cardiovascular function and regeneration we here sought to determine if basic features of SR calcium release are phylogenetically conserved. Confocal calcium imaging was used to detect spontaneous calcium release (calcium sparks and waves) from the SR. Calcium sparks were detected in 16 of 38 trout atrial myocytes and 6 of 15 ventricular cells. The spark amplitude was 1.45±0.03 times the baseline fluorescence and the time to half maximal decay of sparks was 27±3 ms. Spark frequency was 0.88 sparks µm(-1) min(-1) while calcium waves were 8.5 times less frequent. Inhibition of SR calcium uptake reduced the calcium transient (F/F(0)) from 1.77±0.17 to 1.12±0.18 (p = 0.002) and abolished calcium sparks and waves. Moreover, elevation of extracellular calcium from 2 to 10 mM promoted early and delayed afterdepolarizations (from 0.6±0.3 min(-1) to 8.1±2.0 min(-1), p = 0.001), demonstrating the ability of SR calcium release to induce afterdepolarizations in the trout heart. Calcium sparks of similar width and duration were also observed in zebrafish ventricular myocytes. In conclusion, this is the first study to consistently report calcium sparks in teleosts and demonstrate that the basic features of calcium release through the ryanodine receptor are conserved, suggesting that teleost cardiac myocytes is a relevant model to study the functional impact of abnormal SR function.
format	article
author	Anna Llach Cristina E Molina Enrique Alvarez-Lacalle Lluis Tort Raul Benítez Leif Hove-Madsen
author_facet	Anna Llach Cristina E Molina Enrique Alvarez-Lacalle Lluis Tort Raul Benítez Leif Hove-Madsen
author_sort	Anna Llach
title	Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
title_short	Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
title_full	Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
title_fullStr	Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
title_full_unstemmed	Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
title_sort	detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c737d8d9e9b44a088cc37ae101487794
work_keys_str_mv	AT annallach detectionpropertiesandfrequencyoflocalcalciumreleasefromthesarcoplasmicreticuluminteleostcardiomyocytes AT cristinaemolina detectionpropertiesandfrequencyoflocalcalciumreleasefromthesarcoplasmicreticuluminteleostcardiomyocytes AT enriquealvarezlacalle detectionpropertiesandfrequencyoflocalcalciumreleasefromthesarcoplasmicreticuluminteleostcardiomyocytes AT lluistort detectionpropertiesandfrequencyoflocalcalciumreleasefromthesarcoplasmicreticuluminteleostcardiomyocytes AT raulbenitez detectionpropertiesandfrequencyoflocalcalciumreleasefromthesarcoplasmicreticuluminteleostcardiomyocytes AT leifhovemadsen detectionpropertiesandfrequencyoflocalcalciumreleasefromthesarcoplasmicreticuluminteleostcardiomyocytes
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Detection, properties, and frequency of local calcium release from the sarcoplasmic reticulum in teleost cardiomyocytes.

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