Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection

Sharif M Abdelghany,1,&sect; Derek J Quinn,2,&sect; Rebecca J Ingram,2 Brendan F Gilmore,1 Ryan F Donnelly,1 Clifford C Taggart,2 Christopher J Scott11School of Pharmacy, 2Centre for Infection and Immunity, Queen&#39;s University Belfast, UK &sect;These authors contri...

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Autores principales:	Abdelghany SM, Quinn DJ, Ingram RJ, Gilmore BF, Donnelly RF, Taggart CC, Scott CJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c7385e51e2ad43048934ff585b8eabad
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spelling	oai:doaj.org-article:c7385e51e2ad43048934ff585b8eabad2021-12-02T02:48:32ZGentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection1176-91141178-2013https://doaj.org/article/c7385e51e2ad43048934ff585b8eabad2012-07-01T00:00:00Zhttp://www.dovepress.com/gentamicin-loaded-nanoparticles-show-improved-antimicrobial-effects-to-a10516https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Sharif M Abdelghany,1,&sect; Derek J Quinn,2,&sect; Rebecca J Ingram,2 Brendan F Gilmore,1 Ryan F Donnelly,1 Clifford C Taggart,2 Christopher J Scott11School of Pharmacy, 2Centre for Infection and Immunity, Queen&#39;s University Belfast, UK &sect;These authors contributed equally to this workAbstract: Gentamicin is an aminoglycoside antibiotic commonly used for treating Pseudomonas infections, but its use is limited by a relatively short half-life. In this investigation, developed a controlled-release gentamicin formulation using poly(lactide-co-glycolide) (PLGA) nanoparticles. We demonstrate that entrapment of the hydrophilic drug into a hydrophobic PLGA polymer can be improved by increasing the pH of the formulation, reducing the hydrophilicity of the drug and thus enhancing entrapment, achieving levels of up to 22.4 &micro;g/mg PLGA. Under standard incubation conditions, these particles exhibited controlled release of gentamicin for up to 16 days. These particles were tested against both planktonic and biofilm cultures of P. aeruginosa PA01 in vitro, as well as in a 96-hour peritoneal murine infection model. In this model, the particles elicited significantly improved antimicrobial effects as determined by lower plasma and peritoneal lavage colony-forming units and corresponding reductions of the surrogate inflammatory indicators interleukin-6 and myeloperoxidase compared to free drug administration by 96 hours. These data highlight that the controlled release of gentamicin may be applicable for treating Pseudomonas infections.Keywords: anti-microbial, gentamicin, PLGA nanoparticles, Pseudomonas aeruginosaAbdelghany SMQuinn DJIngram RJGilmore BFDonnelly RFTaggart CCScott CJDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4053-4063 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Abdelghany SM Quinn DJ Ingram RJ Gilmore BF Donnelly RF Taggart CC Scott CJ Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection
description	Sharif M Abdelghany,1,&sect; Derek J Quinn,2,&sect; Rebecca J Ingram,2 Brendan F Gilmore,1 Ryan F Donnelly,1 Clifford C Taggart,2 Christopher J Scott11School of Pharmacy, 2Centre for Infection and Immunity, Queen&#39;s University Belfast, UK &sect;These authors contributed equally to this workAbstract: Gentamicin is an aminoglycoside antibiotic commonly used for treating Pseudomonas infections, but its use is limited by a relatively short half-life. In this investigation, developed a controlled-release gentamicin formulation using poly(lactide-co-glycolide) (PLGA) nanoparticles. We demonstrate that entrapment of the hydrophilic drug into a hydrophobic PLGA polymer can be improved by increasing the pH of the formulation, reducing the hydrophilicity of the drug and thus enhancing entrapment, achieving levels of up to 22.4 &micro;g/mg PLGA. Under standard incubation conditions, these particles exhibited controlled release of gentamicin for up to 16 days. These particles were tested against both planktonic and biofilm cultures of P. aeruginosa PA01 in vitro, as well as in a 96-hour peritoneal murine infection model. In this model, the particles elicited significantly improved antimicrobial effects as determined by lower plasma and peritoneal lavage colony-forming units and corresponding reductions of the surrogate inflammatory indicators interleukin-6 and myeloperoxidase compared to free drug administration by 96 hours. These data highlight that the controlled release of gentamicin may be applicable for treating Pseudomonas infections.Keywords: anti-microbial, gentamicin, PLGA nanoparticles, Pseudomonas aeruginosa
format	article
author	Abdelghany SM Quinn DJ Ingram RJ Gilmore BF Donnelly RF Taggart CC Scott CJ
author_facet	Abdelghany SM Quinn DJ Ingram RJ Gilmore BF Donnelly RF Taggart CC Scott CJ
author_sort	Abdelghany SM
title	Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection
title_short	Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection
title_full	Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection
title_fullStr	Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection
title_full_unstemmed	Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection
title_sort	gentamicin-loaded nanoparticles show improved antimicrobial effects towards pseudomonas aeruginosa infection
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/c7385e51e2ad43048934ff585b8eabad
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_version_	1718402125210320896

Gentamicin-loaded nanoparticles show improved antimicrobial effects towards Pseudomonas aeruginosa infection

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