Concept and principles of development of a new generation symbiotic preparation

The article presents the results of the research on the cultivation of B. bifidum and B. subtillis in a high-carbohydrate nutrient medium composed of beet molasses, obtaining metabolites and scientific and practical substantiation of the resulting culture liquid as a biologically active additive. Th...

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Autores principales:	Popov Viktor, Svazlyan Gayane
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/c7422bc690234eae9b2e2a59dc797ece
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spelling	oai:doaj.org-article:c7422bc690234eae9b2e2a59dc797ece2021-11-08T15:17:31ZConcept and principles of development of a new generation symbiotic preparation2117-445810.1051/bioconf/20213700127https://doaj.org/article/c7422bc690234eae9b2e2a59dc797ece2021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/09/bioconf_fies2021_00127.pdfhttps://doaj.org/toc/2117-4458The article presents the results of the research on the cultivation of B. bifidum and B. subtillis in a high-carbohydrate nutrient medium composed of beet molasses, obtaining metabolites and scientific and practical substantiation of the resulting culture liquid as a biologically active additive. The material of the laboratory studies was sugar beet molasses of 5%-10%-15%-20%-25% concentrations. The studies used the probiotic microorganism B. bifidum, strain No. 1 and B. subtilis, strain DSM-3242. To obtain bacterial concentrate, a daily culture of microorganisms was used, which was standardized to 1×106 CFU / cm3. The testing process was carried out according to the indicators of the mass fraction of fermented sugars, determined by the calculation method, branch standard OCT 18-395-82. The number of CFU was calculated in the Goryaev chamber using the method of GPhA (General Pharmacopeic Article) 1.7.2.0008.15. The content of amino acids, vitamins, and organic acids in the culture fluid was determined by capillary electrophoresis in the device “Drops”. During the cultivation of B. bifidum and B. subtillis, a certain metabolic activity of their vitality was found, which is expressed in a decrease in the mass fraction of the amount of fermented sugars in the range of 53.7% to 23.7%. The hydrogen index is from 6.70 to 3.77 in experimental samples of molasses. The number of B. bifidum and B. subtillis CFUs cultivated on 5% molasses on the second day reached 7x106 CFU/cm3 and 8.2 × 106 cm3. In other concentrations, a maximum indicator of the number of microorganisms occurred on the third day and did not exceed 6.6 and 7 × 106 CFU/cm3. A significant synthesis of metabolites in the form of indispensable and conditionally dispensable amino acids and organic acids was determined. The dynamics of amino acid synthesis is consistent with the amylolytic and enzymatic activity of microorganisms in the molasses culture medium. In this case the level of enzymes is directly dependent on the number of CFU.Popov ViktorSvazlyan GayaneEDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 37, p 00127 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Popov Viktor Svazlyan Gayane Concept and principles of development of a new generation symbiotic preparation
description	The article presents the results of the research on the cultivation of B. bifidum and B. subtillis in a high-carbohydrate nutrient medium composed of beet molasses, obtaining metabolites and scientific and practical substantiation of the resulting culture liquid as a biologically active additive. The material of the laboratory studies was sugar beet molasses of 5%-10%-15%-20%-25% concentrations. The studies used the probiotic microorganism B. bifidum, strain No. 1 and B. subtilis, strain DSM-3242. To obtain bacterial concentrate, a daily culture of microorganisms was used, which was standardized to 1×106 CFU / cm3. The testing process was carried out according to the indicators of the mass fraction of fermented sugars, determined by the calculation method, branch standard OCT 18-395-82. The number of CFU was calculated in the Goryaev chamber using the method of GPhA (General Pharmacopeic Article) 1.7.2.0008.15. The content of amino acids, vitamins, and organic acids in the culture fluid was determined by capillary electrophoresis in the device “Drops”. During the cultivation of B. bifidum and B. subtillis, a certain metabolic activity of their vitality was found, which is expressed in a decrease in the mass fraction of the amount of fermented sugars in the range of 53.7% to 23.7%. The hydrogen index is from 6.70 to 3.77 in experimental samples of molasses. The number of B. bifidum and B. subtillis CFUs cultivated on 5% molasses on the second day reached 7x106 CFU/cm3 and 8.2 × 106 cm3. In other concentrations, a maximum indicator of the number of microorganisms occurred on the third day and did not exceed 6.6 and 7 × 106 CFU/cm3. A significant synthesis of metabolites in the form of indispensable and conditionally dispensable amino acids and organic acids was determined. The dynamics of amino acid synthesis is consistent with the amylolytic and enzymatic activity of microorganisms in the molasses culture medium. In this case the level of enzymes is directly dependent on the number of CFU.
format	article
author	Popov Viktor Svazlyan Gayane
author_facet	Popov Viktor Svazlyan Gayane
author_sort	Popov Viktor
title	Concept and principles of development of a new generation symbiotic preparation
title_short	Concept and principles of development of a new generation symbiotic preparation
title_full	Concept and principles of development of a new generation symbiotic preparation
title_fullStr	Concept and principles of development of a new generation symbiotic preparation
title_full_unstemmed	Concept and principles of development of a new generation symbiotic preparation
title_sort	concept and principles of development of a new generation symbiotic preparation
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/c7422bc690234eae9b2e2a59dc797ece
work_keys_str_mv	AT popovviktor conceptandprinciplesofdevelopmentofanewgenerationsymbioticpreparation AT svazlyangayane conceptandprinciplesofdevelopmentofanewgenerationsymbioticpreparation
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Concept and principles of development of a new generation symbiotic preparation

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