Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination

Abstract Intense electromagnetic fields (EMFs) induce DNA double stranded breaks (DSBs) in exposed lymphocytes.We study developing pre-B lymphocytes following V(D)J recombination at their Immunoglobulin light chain loci (IgL). Recombination physiologically induces DNA DSBs, and we tested if low dose...

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Autores principales:	Elena Ioniţă, Aurelian Marcu, Mihaela Temelie, Diana Savu, Mihai Şerbănescu, Mihai Ciubotaru
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c74845ad604346e3a029f25e1d405717
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spelling	oai:doaj.org-article:c74845ad604346e3a029f25e1d4057172021-12-02T16:04:22ZRadiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination10.1038/s41598-021-91790-32045-2322https://doaj.org/article/c74845ad604346e3a029f25e1d4057172021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91790-3https://doaj.org/toc/2045-2322Abstract Intense electromagnetic fields (EMFs) induce DNA double stranded breaks (DSBs) in exposed lymphocytes.We study developing pre-B lymphocytes following V(D)J recombination at their Immunoglobulin light chain loci (IgL). Recombination physiologically induces DNA DSBs, and we tested if low doses of EMF irradiation affect this developmental stage. Recombining pre-B cells, were exposed for 48 h to low intensity EMFs (maximal radiative power density flux S of 9.5 µW/cm2 and electric field intensity 3 V/m) from waves of frequencies ranging from 720 to 1224 MHz. Irradiated pre-B cells show decreased levels of recombination, reduction which is dependent upon the power dose and most remarkably upon the frequency of the applied EMF. Although 50% recombination reduction cannot be obtained even for an S of 9.5 µW/cm2 in cells irradiated at 720 MHz, such an effect is reached in cells exposed to only 0.45 µW/cm2 power with 950 and 1000 MHz waves. A maximal four-fold recombination reduction was measured in cells exposed to 1000 MHz waves with S from 0.2 to 4.5 µW/cm2 displaying normal levels of γH2AX phosphorylated histone. Our findings show that developing B cells exposure to low intensity EMFs can affect the levels of production and diversity of their antibodies repertoire.Elena IoniţăAurelian MarcuMihaela TemelieDiana SavuMihai ŞerbănescuMihai CiubotaruNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena Ioniţă Aurelian Marcu Mihaela Temelie Diana Savu Mihai Şerbănescu Mihai Ciubotaru Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination
description	Abstract Intense electromagnetic fields (EMFs) induce DNA double stranded breaks (DSBs) in exposed lymphocytes.We study developing pre-B lymphocytes following V(D)J recombination at their Immunoglobulin light chain loci (IgL). Recombination physiologically induces DNA DSBs, and we tested if low doses of EMF irradiation affect this developmental stage. Recombining pre-B cells, were exposed for 48 h to low intensity EMFs (maximal radiative power density flux S of 9.5 µW/cm2 and electric field intensity 3 V/m) from waves of frequencies ranging from 720 to 1224 MHz. Irradiated pre-B cells show decreased levels of recombination, reduction which is dependent upon the power dose and most remarkably upon the frequency of the applied EMF. Although 50% recombination reduction cannot be obtained even for an S of 9.5 µW/cm2 in cells irradiated at 720 MHz, such an effect is reached in cells exposed to only 0.45 µW/cm2 power with 950 and 1000 MHz waves. A maximal four-fold recombination reduction was measured in cells exposed to 1000 MHz waves with S from 0.2 to 4.5 µW/cm2 displaying normal levels of γH2AX phosphorylated histone. Our findings show that developing B cells exposure to low intensity EMFs can affect the levels of production and diversity of their antibodies repertoire.
format	article
author	Elena Ioniţă Aurelian Marcu Mihaela Temelie Diana Savu Mihai Şerbănescu Mihai Ciubotaru
author_facet	Elena Ioniţă Aurelian Marcu Mihaela Temelie Diana Savu Mihai Şerbănescu Mihai Ciubotaru
author_sort	Elena Ioniţă
title	Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination
title_short	Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination
title_full	Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination
title_fullStr	Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination
title_full_unstemmed	Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination
title_sort	radiofrequency emf irradiation effects on pre-b lymphocytes undergoing somatic recombination
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c74845ad604346e3a029f25e1d405717
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Radiofrequency EMF irradiation effects on pre-B lymphocytes undergoing somatic recombination

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