Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation

Abstract Accumulating evidence suggests that modifications of gut function and microbiota composition might play a pivotal role in the pathophysiology of several cardiovascular diseases, including heart failure (HF). In this study we systematically analysed gut microbiota composition, intestinal bar...

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Autores principales:	Nicola Boccella, Roberta Paolillo, Lorena Coretti, Stefania D’Apice, Adriano Lama, Giuseppe Giugliano, Gabriele Giacomo Schiattarella, Mariella Cuomo, Ilaria d’Aquino, Gina Cavaliere, Orlando Paciello, Maria Pina Mollica, Giuseppina Mattace Raso, Giovanni Esposito, Francesca Lembo, Cinzia Perrino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c74fa22f500346549147bf13cc6d2327
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spelling	oai:doaj.org-article:c74fa22f500346549147bf13cc6d23272021-12-02T14:23:23ZTransverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation10.1038/s41598-021-86651-y2045-2322https://doaj.org/article/c74fa22f500346549147bf13cc6d23272021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86651-yhttps://doaj.org/toc/2045-2322Abstract Accumulating evidence suggests that modifications of gut function and microbiota composition might play a pivotal role in the pathophysiology of several cardiovascular diseases, including heart failure (HF). In this study we systematically analysed gut microbiota composition, intestinal barrier integrity, intestinal and serum cytokines and serum endotoxin levels in C57BL/6 mice undergoing pressure overload by transverse aortic constriction (TAC) for 1 and 4 weeks. Compared to sham-operated animals, TAC induced prompt and strong weakening of intestinal barrier integrity, long-lasting decrease of colon anti-inflammatory cytokine levels, significant increases of serum levels of bacterial lipopolysaccharide and proinflammatory cytokines. TAC also exerted effects on microbiota composition, inducing significant differences in bacterial genera inside Actinobacteria, Firmicutes, Proteobacteria and TM7 phyla as shown by 16S rDNA sequencing of fecal samples from TAC or sham mice. These results suggest that gut modifications represent an important element to be considered in the development and progression of cardiac dysfunction in response to TAC and support this animal model as a valuable tool to establish the role and mechanisms of gut-heart crosstalk in HF. Evidence arising in this field might identify new treatment options targeting gut integrity and microbiota components to face adverse cardiac events.Nicola BoccellaRoberta PaolilloLorena CorettiStefania D’ApiceAdriano LamaGiuseppe GiuglianoGabriele Giacomo SchiattarellaMariella CuomoIlaria d’AquinoGina CavaliereOrlando PacielloMaria Pina MollicaGiuseppina Mattace RasoGiovanni EspositoFrancesca LemboCinzia PerrinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nicola Boccella Roberta Paolillo Lorena Coretti Stefania D’Apice Adriano Lama Giuseppe Giugliano Gabriele Giacomo Schiattarella Mariella Cuomo Ilaria d’Aquino Gina Cavaliere Orlando Paciello Maria Pina Mollica Giuseppina Mattace Raso Giovanni Esposito Francesca Lembo Cinzia Perrino Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
description	Abstract Accumulating evidence suggests that modifications of gut function and microbiota composition might play a pivotal role in the pathophysiology of several cardiovascular diseases, including heart failure (HF). In this study we systematically analysed gut microbiota composition, intestinal barrier integrity, intestinal and serum cytokines and serum endotoxin levels in C57BL/6 mice undergoing pressure overload by transverse aortic constriction (TAC) for 1 and 4 weeks. Compared to sham-operated animals, TAC induced prompt and strong weakening of intestinal barrier integrity, long-lasting decrease of colon anti-inflammatory cytokine levels, significant increases of serum levels of bacterial lipopolysaccharide and proinflammatory cytokines. TAC also exerted effects on microbiota composition, inducing significant differences in bacterial genera inside Actinobacteria, Firmicutes, Proteobacteria and TM7 phyla as shown by 16S rDNA sequencing of fecal samples from TAC or sham mice. These results suggest that gut modifications represent an important element to be considered in the development and progression of cardiac dysfunction in response to TAC and support this animal model as a valuable tool to establish the role and mechanisms of gut-heart crosstalk in HF. Evidence arising in this field might identify new treatment options targeting gut integrity and microbiota components to face adverse cardiac events.
format	article
author	Nicola Boccella Roberta Paolillo Lorena Coretti Stefania D’Apice Adriano Lama Giuseppe Giugliano Gabriele Giacomo Schiattarella Mariella Cuomo Ilaria d’Aquino Gina Cavaliere Orlando Paciello Maria Pina Mollica Giuseppina Mattace Raso Giovanni Esposito Francesca Lembo Cinzia Perrino
author_facet	Nicola Boccella Roberta Paolillo Lorena Coretti Stefania D’Apice Adriano Lama Giuseppe Giugliano Gabriele Giacomo Schiattarella Mariella Cuomo Ilaria d’Aquino Gina Cavaliere Orlando Paciello Maria Pina Mollica Giuseppina Mattace Raso Giovanni Esposito Francesca Lembo Cinzia Perrino
author_sort	Nicola Boccella
title	Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
title_short	Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
title_full	Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
title_fullStr	Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
title_full_unstemmed	Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
title_sort	transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c74fa22f500346549147bf13cc6d2327
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Transverse aortic constriction induces gut barrier alterations, microbiota remodeling and systemic inflammation

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