Soft robotic constrictor for in vitro modeling of dynamic tissue compression

Abstract Here we present a microengineered soft-robotic in vitro platform developed by integrating a pneumatically regulated novel elastomeric actuator with primary culture of human cells. This system is capable of generating dynamic bending motion akin to the constriction of tubular organs that can...

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Autores principales:	Jungwook Paek, Joseph W. Song, Ehsan Ban, Yuma Morimitsu, Chinedum O. Osuji, Vivek B. Shenoy, Dan Dongeun Huh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7504db7e2874ba9bc76eb5d72543068
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spelling	oai:doaj.org-article:c7504db7e2874ba9bc76eb5d725430682021-12-02T16:43:39ZSoft robotic constrictor for in vitro modeling of dynamic tissue compression10.1038/s41598-021-94769-22045-2322https://doaj.org/article/c7504db7e2874ba9bc76eb5d725430682021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94769-2https://doaj.org/toc/2045-2322Abstract Here we present a microengineered soft-robotic in vitro platform developed by integrating a pneumatically regulated novel elastomeric actuator with primary culture of human cells. This system is capable of generating dynamic bending motion akin to the constriction of tubular organs that can exert controlled compressive forces on cultured living cells. Using this platform, we demonstrate cyclic compression of primary human endothelial cells, fibroblasts, and smooth muscle cells to show physiological changes in their morphology due to applied forces. Moreover, we present mechanically actuatable organotypic models to examine the effects of compressive forces on three-dimensional multicellular constructs designed to emulate complex tissues such as solid tumors and vascular networks. Our work provides a preliminary demonstration of how soft-robotics technology can be leveraged for in vitro modeling of complex physiological tissue microenvironment, and may enable the development of new research tools for mechanobiology and related areas.Jungwook PaekJoseph W. SongEhsan BanYuma MorimitsuChinedum O. OsujiVivek B. ShenoyDan Dongeun HuhNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jungwook Paek Joseph W. Song Ehsan Ban Yuma Morimitsu Chinedum O. Osuji Vivek B. Shenoy Dan Dongeun Huh Soft robotic constrictor for in vitro modeling of dynamic tissue compression
description	Abstract Here we present a microengineered soft-robotic in vitro platform developed by integrating a pneumatically regulated novel elastomeric actuator with primary culture of human cells. This system is capable of generating dynamic bending motion akin to the constriction of tubular organs that can exert controlled compressive forces on cultured living cells. Using this platform, we demonstrate cyclic compression of primary human endothelial cells, fibroblasts, and smooth muscle cells to show physiological changes in their morphology due to applied forces. Moreover, we present mechanically actuatable organotypic models to examine the effects of compressive forces on three-dimensional multicellular constructs designed to emulate complex tissues such as solid tumors and vascular networks. Our work provides a preliminary demonstration of how soft-robotics technology can be leveraged for in vitro modeling of complex physiological tissue microenvironment, and may enable the development of new research tools for mechanobiology and related areas.
format	article
author	Jungwook Paek Joseph W. Song Ehsan Ban Yuma Morimitsu Chinedum O. Osuji Vivek B. Shenoy Dan Dongeun Huh
author_facet	Jungwook Paek Joseph W. Song Ehsan Ban Yuma Morimitsu Chinedum O. Osuji Vivek B. Shenoy Dan Dongeun Huh
author_sort	Jungwook Paek
title	Soft robotic constrictor for in vitro modeling of dynamic tissue compression
title_short	Soft robotic constrictor for in vitro modeling of dynamic tissue compression
title_full	Soft robotic constrictor for in vitro modeling of dynamic tissue compression
title_fullStr	Soft robotic constrictor for in vitro modeling of dynamic tissue compression
title_full_unstemmed	Soft robotic constrictor for in vitro modeling of dynamic tissue compression
title_sort	soft robotic constrictor for in vitro modeling of dynamic tissue compression
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/c7504db7e2874ba9bc76eb5d72543068
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Soft robotic constrictor for in vitro modeling of dynamic tissue compression

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