lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3

AIM: To investigate whether long-chain non-coding RNA(lncRNA)KCNQ1OT1 affects the proliferation, apoptosis and oxidative stress of retinal epithelial cells induced by high glucose(HG)through miR-19a-3p/TSHZ3. METHODS: Cell counting kit 8(CCK-8)was used to detect the cell viability of human retinal e...

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Autores principales:	Ni-Hong Zhang, Ding-Shan Hou, Jian-Zhi Qiao, Ke Fu, Hong-Liang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Press of International Journal of Ophthalmology (IJO PRESS) 2021
Materias:	diabetic retinopathy lncrna kcnq1ot1 mir-19a-3p tshz3 proliferation apoptosis oxidative stress Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/c756716548934c6e8ea61ded2a8973fe
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spelling	oai:doaj.org-article:c756716548934c6e8ea61ded2a8973fe2021-11-22T14:22:32ZlncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ31672-512310.3980/j.issn.1672-5123.2021.12.05https://doaj.org/article/c756716548934c6e8ea61ded2a8973fe2021-12-01T00:00:00Zhttp://ies.ijo.cn/cn_publish/2021/12/202112005.pdfhttps://doaj.org/toc/1672-5123AIM: To investigate whether long-chain non-coding RNA(lncRNA)KCNQ1OT1 affects the proliferation, apoptosis and oxidative stress of retinal epithelial cells induced by high glucose(HG)through miR-19a-3p/TSHZ3. METHODS: Cell counting kit 8(CCK-8)was used to detect the cell viability of human retinal epithelial cells ARPE-19 stimulated with 5, 15, 45, 135mmol/L HG. The ARPE-19 cells were divided into NC group, 45mmol/L HG group, si-NC+45mmol/L HG group, si-lncRNA KCNQ1OT1+45mmol/L HG group, miR-NC+45mmol/L HG group, miR-19a-3p mimics+45mmol/L HG group, si-con+45mmol/L HG group, si-TSHZ3+45mmol/L HG group, pcDNA+si-lncRNA KCNQ1OT1+45mmol/L HG group, pcDNA-TSHZ3+si-lncRNA KCNQ1OT1+45mmol/L HG group. CCK-8 was used to detect cell viability, qRT-PCR was used to detect the expressions of lncRNA KCNQ1OT1, miR-19a-3p and TSHZ3 mRNA, Western Blot was used to detect TSHZ3, activation-cysteine-containing aspartate proteolytic enzyme 3(Cleaved-caspase-3), B-cell lymphoma/leukemia-2(Bcl-2)related X protein(Bax)protein expressions, and enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of oxidative stress indicators reactive oxygen species(ROS)and malondialdehyde(MDA). The dual luciferase activity was used to detect the targeted binding between lncRNA KCNQ1OT1 and miR-19a-3p, miR-19a-3p and TSHZ3. RESULTS: 15, 45, 135mmol/L HG inhibited the survival rate of ARPE-19 cells, and the subsequent select the HG concentration 45mmol/L with a cell survival rate of about 50%. 45mmol/L HG increased the expression levels of lncRNA KCNQ1OT1, TSHZ3 mRNA, TSHZ3 protein, the apoptosis rate, Cleaved-caspase-3 and Bax protein expressions, ROS and MDA levels in ARPE-19 cells, and reduced cell survival rate and the expression level of miR-19a-3p(P<0.05). Low expression of lncRNA KCNQ1OT1, TSHZ3 or high expression of miR-19a-3p improved the survival rate of ARPE-19 cells induced by HG, and reduced apoptosis rate, Cleaved-caspase-3 and Bax protein expressions, ROS and MDA levels(P<0.05). lncRNA KCNQ1OT1 targeted miR-19a-3p, miR-19a-3p targeted TSHZ3, and lncRNA KCNQ1OT13 regulated the expression of TSHZ3 through miR-19a-3p. The effect of lncRNA KCNQ1OT1 low expression on the survival rate, apoptosis and oxidative stress of ARPE-19 cells induced by HG was reversed by the overexpression of TSHZ3.CONCLUSION: The low expression of lncRNA KCNQ1OT13 promotes the proliferation of retinal epithelial cells induced by high glucose, and inhibits their apoptosis and oxidative stress through miR-19a-3p/TSHZ3.Ni-Hong ZhangDing-Shan HouJian-Zhi QiaoKe FuHong-Liang WangPress of International Journal of Ophthalmology (IJO PRESS)articlediabetic retinopathylncrna kcnq1ot1mir-19a-3ptshz3proliferationapoptosisoxidative stressOphthalmologyRE1-994ENGuoji Yanke Zazhi, Vol 21, Iss 12, Pp 2044-2052 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic retinopathy lncrna kcnq1ot1 mir-19a-3p tshz3 proliferation apoptosis oxidative stress Ophthalmology RE1-994
spellingShingle	diabetic retinopathy lncrna kcnq1ot1 mir-19a-3p tshz3 proliferation apoptosis oxidative stress Ophthalmology RE1-994 Ni-Hong Zhang Ding-Shan Hou Jian-Zhi Qiao Ke Fu Hong-Liang Wang lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3
description	AIM: To investigate whether long-chain non-coding RNA(lncRNA)KCNQ1OT1 affects the proliferation, apoptosis and oxidative stress of retinal epithelial cells induced by high glucose(HG)through miR-19a-3p/TSHZ3. METHODS: Cell counting kit 8(CCK-8)was used to detect the cell viability of human retinal epithelial cells ARPE-19 stimulated with 5, 15, 45, 135mmol/L HG. The ARPE-19 cells were divided into NC group, 45mmol/L HG group, si-NC+45mmol/L HG group, si-lncRNA KCNQ1OT1+45mmol/L HG group, miR-NC+45mmol/L HG group, miR-19a-3p mimics+45mmol/L HG group, si-con+45mmol/L HG group, si-TSHZ3+45mmol/L HG group, pcDNA+si-lncRNA KCNQ1OT1+45mmol/L HG group, pcDNA-TSHZ3+si-lncRNA KCNQ1OT1+45mmol/L HG group. CCK-8 was used to detect cell viability, qRT-PCR was used to detect the expressions of lncRNA KCNQ1OT1, miR-19a-3p and TSHZ3 mRNA, Western Blot was used to detect TSHZ3, activation-cysteine-containing aspartate proteolytic enzyme 3(Cleaved-caspase-3), B-cell lymphoma/leukemia-2(Bcl-2)related X protein(Bax)protein expressions, and enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of oxidative stress indicators reactive oxygen species(ROS)and malondialdehyde(MDA). The dual luciferase activity was used to detect the targeted binding between lncRNA KCNQ1OT1 and miR-19a-3p, miR-19a-3p and TSHZ3. RESULTS: 15, 45, 135mmol/L HG inhibited the survival rate of ARPE-19 cells, and the subsequent select the HG concentration 45mmol/L with a cell survival rate of about 50%. 45mmol/L HG increased the expression levels of lncRNA KCNQ1OT1, TSHZ3 mRNA, TSHZ3 protein, the apoptosis rate, Cleaved-caspase-3 and Bax protein expressions, ROS and MDA levels in ARPE-19 cells, and reduced cell survival rate and the expression level of miR-19a-3p(P<0.05). Low expression of lncRNA KCNQ1OT1, TSHZ3 or high expression of miR-19a-3p improved the survival rate of ARPE-19 cells induced by HG, and reduced apoptosis rate, Cleaved-caspase-3 and Bax protein expressions, ROS and MDA levels(P<0.05). lncRNA KCNQ1OT1 targeted miR-19a-3p, miR-19a-3p targeted TSHZ3, and lncRNA KCNQ1OT13 regulated the expression of TSHZ3 through miR-19a-3p. The effect of lncRNA KCNQ1OT1 low expression on the survival rate, apoptosis and oxidative stress of ARPE-19 cells induced by HG was reversed by the overexpression of TSHZ3.CONCLUSION: The low expression of lncRNA KCNQ1OT13 promotes the proliferation of retinal epithelial cells induced by high glucose, and inhibits their apoptosis and oxidative stress through miR-19a-3p/TSHZ3.
format	article
author	Ni-Hong Zhang Ding-Shan Hou Jian-Zhi Qiao Ke Fu Hong-Liang Wang
author_facet	Ni-Hong Zhang Ding-Shan Hou Jian-Zhi Qiao Ke Fu Hong-Liang Wang
author_sort	Ni-Hong Zhang
title	lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3
title_short	lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3
title_full	lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3
title_fullStr	lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3
title_full_unstemmed	lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3
title_sort	lncrna kcnq1ot1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through mir-19a-3p/tshz3
publisher	Press of International Journal of Ophthalmology (IJO PRESS)
publishDate	2021
url	https://doaj.org/article/c756716548934c6e8ea61ded2a8973fe
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lncRNA KCNQ1OT1 affects the proliferation and apoptosis of retinal epithelial cells induced by high glucose through miR-19a-3p/TSHZ3

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