Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

Hisao Haniu,1,2 Naoto Saito,2,3 Yoshikazu Matsuda,4 Tamotsu Tsukahara,5 Yuki Usui,1,6,7 Kayo Maruyama,2,3 Seiji Takanashi,1 Kaoru Aoki,1 Shinsuke Kobayashi,1 Hiroki Nomura,1 Manabu Tanaka,1 Masanori Okamoto,1 Hiroyuki Kato1 1Department of Orthopaedic Surgery, Shinshu University School of Medicine,...

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Autores principales:	Haniu H, Saito N, Matsuda Y, Tsukahara T, Usui Y, Maruyama K, Takanashi S, Aoki K, Kobayashi S, Nomura H, Tanaka M, Okamoto M, Kato H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/c75b2649b6574e0eb415ab2dfbccaa1d
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spelling	oai:doaj.org-article:c75b2649b6574e0eb415ab2dfbccaa1d2021-12-02T02:11:14ZBiological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells1178-2013https://doaj.org/article/c75b2649b6574e0eb415ab2dfbccaa1d2014-04-01T00:00:00Zhttp://www.dovepress.com/biological-responses-according-to-the-shape-and-size-of-carbon-nanotub-a16508https://doaj.org/toc/1178-2013 Hisao Haniu,1,2 Naoto Saito,2,3 Yoshikazu Matsuda,4 Tamotsu Tsukahara,5 Yuki Usui,1,6,7 Kayo Maruyama,2,3 Seiji Takanashi,1 Kaoru Aoki,1 Shinsuke Kobayashi,1 Hiroki Nomura,1 Manabu Tanaka,1 Masanori Okamoto,1 Hiroyuki Kato1 1Department of Orthopaedic Surgery, Shinshu University School of Medicine, Nagano, Japan; 2Insutitute for Biomedical Sciences, Shinshu University, Nagano, Japan; 3Department of Applied Physical Therapy, Shinshu University School of Health Sciences, Nagano, Japan; 4Clinical Pharmacology Educational Center, Nihon Pharmaceutical University, Saitama, Japan; 5Department of Hematology and Immunology, Kanazawa Medical University, Ishikawa, Japan; 6Research Center for Exotic Nanocarbons, Shinshu University, Nagano, Japan; 7Aizawa Hospital, Sports Medicine Center, Nagano, Japan Abstract: This study aimed to investigate the influence of the shape and size of multi-walled carbon nanotubes (MWCNTs) and cup-stacked carbon nanotubes (CSCNTs) on biological responses in vitro. Three types of MWCNTs – VGCF®-X, VGCF®-S, and VGCF® (vapor grown carbon fibers; with diameters of 15, 80, and 150 nm, respectively) – and three CSCNTs of different lengths (CS-L, 20–80 µm; CS-S, 0.5–20 µm; and CS-M, of intermediate length) were tested. Human bronchial epithelial (BEAS-2B) and malignant pleural mesothelioma cells were exposed to the CNTs (1–50 µg/mL), and cell viability, permeability, uptake, total reactive oxygen species/superoxide production, and intracellular acidity were measured. CSCNTs were less toxic than MWCNTs in both cell types over a 24-hour exposure period. The cytotoxicity of endocytosed MWCNTs varied according to cell type/size, while that of CSCNTs depended on tube length irrespective of cell type. CNT diameter and length influenced cell aggregation and injury extent. Intracellular acidity increased independently of lysosomal activity along with the number of vacuoles in BEAS-2B cells exposed for 24 hours to either CNT (concentration, 10 µg/mL). However, total reactive oxygen species/superoxide generation did not contribute to cytotoxicity. The results demonstrate that CSCNTs could be suitable for biological applications and that CNT shape and size can have differential effects depending on cell type, which can be exploited in the development of highly specialized, biocompatible CNTs. Keywords: multi-walled carbon nanotube, cup-stacked carbon nanotube, cytotoxicity, in vitro, intracellular acidityHaniu HSaito NMatsuda YTsukahara TUsui YMaruyama KTakanashi SAoki KKobayashi SNomura HTanaka MOkamoto MKato HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 1979-1990 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Haniu H Saito N Matsuda Y Tsukahara T Usui Y Maruyama K Takanashi S Aoki K Kobayashi S Nomura H Tanaka M Okamoto M Kato H Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells
description	Hisao Haniu,1,2 Naoto Saito,2,3 Yoshikazu Matsuda,4 Tamotsu Tsukahara,5 Yuki Usui,1,6,7 Kayo Maruyama,2,3 Seiji Takanashi,1 Kaoru Aoki,1 Shinsuke Kobayashi,1 Hiroki Nomura,1 Manabu Tanaka,1 Masanori Okamoto,1 Hiroyuki Kato1 1Department of Orthopaedic Surgery, Shinshu University School of Medicine, Nagano, Japan; 2Insutitute for Biomedical Sciences, Shinshu University, Nagano, Japan; 3Department of Applied Physical Therapy, Shinshu University School of Health Sciences, Nagano, Japan; 4Clinical Pharmacology Educational Center, Nihon Pharmaceutical University, Saitama, Japan; 5Department of Hematology and Immunology, Kanazawa Medical University, Ishikawa, Japan; 6Research Center for Exotic Nanocarbons, Shinshu University, Nagano, Japan; 7Aizawa Hospital, Sports Medicine Center, Nagano, Japan Abstract: This study aimed to investigate the influence of the shape and size of multi-walled carbon nanotubes (MWCNTs) and cup-stacked carbon nanotubes (CSCNTs) on biological responses in vitro. Three types of MWCNTs – VGCF®-X, VGCF®-S, and VGCF® (vapor grown carbon fibers; with diameters of 15, 80, and 150 nm, respectively) – and three CSCNTs of different lengths (CS-L, 20–80 µm; CS-S, 0.5–20 µm; and CS-M, of intermediate length) were tested. Human bronchial epithelial (BEAS-2B) and malignant pleural mesothelioma cells were exposed to the CNTs (1–50 µg/mL), and cell viability, permeability, uptake, total reactive oxygen species/superoxide production, and intracellular acidity were measured. CSCNTs were less toxic than MWCNTs in both cell types over a 24-hour exposure period. The cytotoxicity of endocytosed MWCNTs varied according to cell type/size, while that of CSCNTs depended on tube length irrespective of cell type. CNT diameter and length influenced cell aggregation and injury extent. Intracellular acidity increased independently of lysosomal activity along with the number of vacuoles in BEAS-2B cells exposed for 24 hours to either CNT (concentration, 10 µg/mL). However, total reactive oxygen species/superoxide generation did not contribute to cytotoxicity. The results demonstrate that CSCNTs could be suitable for biological applications and that CNT shape and size can have differential effects depending on cell type, which can be exploited in the development of highly specialized, biocompatible CNTs. Keywords: multi-walled carbon nanotube, cup-stacked carbon nanotube, cytotoxicity, in vitro, intracellular acidity
format	article
author	Haniu H Saito N Matsuda Y Tsukahara T Usui Y Maruyama K Takanashi S Aoki K Kobayashi S Nomura H Tanaka M Okamoto M Kato H
author_facet	Haniu H Saito N Matsuda Y Tsukahara T Usui Y Maruyama K Takanashi S Aoki K Kobayashi S Nomura H Tanaka M Okamoto M Kato H
author_sort	Haniu H
title	Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells
title_short	Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells
title_full	Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells
title_fullStr	Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells
title_full_unstemmed	Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells
title_sort	biological responses according to the shape and size of carbon nanotubes in beas-2b and meso-1 cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/c75b2649b6574e0eb415ab2dfbccaa1d
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Biological responses according to the shape and size of carbon nanotubes in BEAS-2B and MESO-1 cells

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