Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.

DNA methylation is widespread in most species, from bacteria to mammals, and is crucial for genomic imprinting, gene expression, and embryogenesis. DNA methylation occurs via two major classes of enzymatic reactions: maintenance-type methylation catalyzed by DNA (cytosine-5-)-methyltransferase (DNMT...

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Autores principales:	Deivendran Rengaraj, Bo Ram Lee, Sang In Lee, Hee Won Seo, Jae Yong Han
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/c7629458f29d4440bf036e4dd03a27d1
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spelling	oai:doaj.org-article:c7629458f29d4440bf036e4dd03a27d12021-11-18T06:54:32ZExpression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.1932-620310.1371/journal.pone.0019524https://doaj.org/article/c7629458f29d4440bf036e4dd03a27d12011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21559294/?tool=EBIhttps://doaj.org/toc/1932-6203DNA methylation is widespread in most species, from bacteria to mammals, and is crucial for genomic imprinting, gene expression, and embryogenesis. DNA methylation occurs via two major classes of enzymatic reactions: maintenance-type methylation catalyzed by DNA (cytosine-5-)-methyltransferase (DNMT) 1, and de novo methylation catalyzed by DNMT 3 alpha (DNMT3A) and -beta (DNMT3B). The expression pattern and regulation of DNMT genes in primordial germ cells (PGCs) and germ line cells has not been sufficiently established in birds. Therefore, we employed bioinformatics, RT-PCR, real-time PCR, and in situ hybridization analyses to examine the structural conservation and conserved expression patterns of chicken DNMT family genes. We further examined the regulation of a candidate de novo DNA methyltransferase gene, cDNMT3B by cotransfection of cDNMT3B 3'UTR- and cDNMT3B 3'UTR-specific miRNAs through a dual fluorescence reporter assay. All cDNMT family members were differentially detected during early embryonic development. Of interest, cDNMT3B expression was highly detected in early embryos and in PGCs. During germ line development and sexual maturation, cDNMT3B expression was reestablished in a female germ cell-specific manner. In the dual fluorescence reporter assay, cDNMT3B expression was significantly downregulated by four miRNAs: gga-miR-15c (25.82%), gga-miR-29b (30.01%), gga-miR-383 (30.0%), and gga-miR-222 (31.28%). Our data highlight the structural conservation and conserved expression patterns of chicken DNMTs. The miRNAs investigated in this study may induce downregulation of gene expression in chicken PGCs and germ cells.Deivendran RengarajBo Ram LeeSang In LeeHee Won SeoJae Yong HanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e19524 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deivendran Rengaraj Bo Ram Lee Sang In Lee Hee Won Seo Jae Yong Han Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
description	DNA methylation is widespread in most species, from bacteria to mammals, and is crucial for genomic imprinting, gene expression, and embryogenesis. DNA methylation occurs via two major classes of enzymatic reactions: maintenance-type methylation catalyzed by DNA (cytosine-5-)-methyltransferase (DNMT) 1, and de novo methylation catalyzed by DNMT 3 alpha (DNMT3A) and -beta (DNMT3B). The expression pattern and regulation of DNMT genes in primordial germ cells (PGCs) and germ line cells has not been sufficiently established in birds. Therefore, we employed bioinformatics, RT-PCR, real-time PCR, and in situ hybridization analyses to examine the structural conservation and conserved expression patterns of chicken DNMT family genes. We further examined the regulation of a candidate de novo DNA methyltransferase gene, cDNMT3B by cotransfection of cDNMT3B 3'UTR- and cDNMT3B 3'UTR-specific miRNAs through a dual fluorescence reporter assay. All cDNMT family members were differentially detected during early embryonic development. Of interest, cDNMT3B expression was highly detected in early embryos and in PGCs. During germ line development and sexual maturation, cDNMT3B expression was reestablished in a female germ cell-specific manner. In the dual fluorescence reporter assay, cDNMT3B expression was significantly downregulated by four miRNAs: gga-miR-15c (25.82%), gga-miR-29b (30.01%), gga-miR-383 (30.0%), and gga-miR-222 (31.28%). Our data highlight the structural conservation and conserved expression patterns of chicken DNMTs. The miRNAs investigated in this study may induce downregulation of gene expression in chicken PGCs and germ cells.
format	article
author	Deivendran Rengaraj Bo Ram Lee Sang In Lee Hee Won Seo Jae Yong Han
author_facet	Deivendran Rengaraj Bo Ram Lee Sang In Lee Hee Won Seo Jae Yong Han
author_sort	Deivendran Rengaraj
title	Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
title_short	Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
title_full	Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
title_fullStr	Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
title_full_unstemmed	Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.
title_sort	expression patterns and mirna regulation of dna methyltransferases in chicken primordial germ cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/c7629458f29d4440bf036e4dd03a27d1
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_version_	1718424255144656896

Expression patterns and miRNA regulation of DNA methyltransferases in chicken primordial germ cells.

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